We narrowed to 883 results for: prot

...plasmids Anti-CRISPR proteins for optogenetic control of CRISPR-Cas9 Anti-CRISPR proteins that inhibit type...Find the plant genome editing toolkit New Cas protein identified Jennifer Doudna’s lab recently identified...identified Cas14, a family of small DNA-targeting Cas proteins. Cas14 targets and cleaves ssDNA which they have...Now Dominik Niopek’s lab has adapted anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. By combining...

... ortholog was fused to a different fluorescent protein to create three colors. The specificity of these...estimate that a minimum of 150-200 fluorescent protein molecules is necessary for a detectable signal,... scaffold that can be used to recruit multiple protein molecules (and also available from Addgene!) Since...plasmids from this publication at Addgene. A protein-tagging system for signal amplification in gene... fluorescent protein...

...discovery of microbial rhodopsins, light-sensitive proteins that absorb photons for energy conversion or the... et al., 1984; Harz and Hegemann, 1991). These proteins control the flow of electric charges across cell...Purification and properties of the Cre recombinase protein." Journal of Biological Chemistry 259.3 (1984):...Dieter, and Walther Stoeckenius. "Rhodopsin-like protein from the purple membrane of Halobacterium halobium...modification technology in the mouse." Current protocols in immunology 105.1 (2014): 10-34. PubMed PMID...clinical applications of optogenetics." Current protocols in pharmacology 75.1 (2016): 11-19. PubMed PMID...

...imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent protein biosensors ...ARGs) are engineered gene clusters that encode proteins that form bacterial gas vesicles. These ~200nm...200nm long vesicles have hollow interiors and a protein shell that excludes water but is gas permeable. These...luciferase reporter gene The green fluorescent protein reporter gene Resources on Addgene.org Find plasmids...

...re-exposure (2, 3). More specifically, specialized Cas proteins snip the foreign DNA into small fragments approximately...paste them into the CRISPR sequence. Separate Cas proteins then express and process the CRISPR loci to generate...genetic material next to the species-specific protospacer adjacent motif (PAM). Once the CRISPR/Cas complex...out these functions using slightly different Cas proteins and PAM sequences. A total of eight evolutionarily...the CRISPR/CAS toolkit by posting generic lab protocols, providing tips from experts in the field, and...

...introductory blog post, then check out this RNP protocol on Protocols.io. The PAM Requirement and Expanding CRISPR...biophysics can help enhance HDR. CRISPR 101: Ribonucleoprotein (RNP) Delivery The RNP delivery method consistently...Channabasavaiah B. Gurumurthy, and Masato Ohtsuka. Nat Protoc. 13(1) (2018):195-215. PubMed PMID: 29266098 Creating...models with CRISPR has been challenging, with protocols using dsDNA templates achieving ~1-10% efficiency...

...antiviral defense) complexes, while Class 2 use single protein Cas effectors, like Cas9. The Cascade complex doesn...effector complex but are defined by the accessory Cas proteins in the Cascade complex. Despite a tenuous grasp... Despite being in Class 1, Cas7-11 is a single protein effector and is able to target and edit RNA in ...Class 2 Class 2 CRISPR systems rely on a single protein Cas effector and are likely the class most people...discovery algorithms find new candidates for CRISPR proteins. A type VII is already on the horizon (Altae-Tran...2021). Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Nature, 597(7878), 720...

...acids (DNA and RNA) absorb maximally at 260 nm. Proteins on the other hand absorb best at 280 nm and organic...can’t distinguish between DNA and RNA. See our protocol about using UV absorbance to quantify DNA Fluorescence...this method doesn’t tell you about contaminating proteins or chaotropic salts. Capillary electrophoresis...Addgene blog Read more about molecular biology protocols and tips Find the best way to elute and store ...Reference Find plasmids for your research Browse protocols for molecular biology, cloning, and more ...

...The catalytically dead Cas9 protein (dCas9) is well known for its ability to bind DNA targets without.... To do so, they fused a different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9...boxB) that each recruits a different fluorescent protein (BFP, GFP, or RFP) to the genomic loci of interest...) showing recruitment of different fluorescent proteins. (B) CRISPR-Sirius used to visualize relative ...Download our CRISPR eBook Learn about fluorescent protein-based tools Read all CRISPR blog posts Resources...

...structured surfaces. Plasmids expressing fluorescent proteins will be used to tag individual strains of bacteria...complications of diabetes mellitus. Recombinant human proteins that play a role in insulin regulation and diabetes...and the activity of the plant compounds on these proteins will be tested. Investigating changes in neuronal...condition is crucial. Plasmids expressing fluorescent proteins and a new two-photon laser scanning microscope...containing promoter regions that activate fluorescent protein transcription in response to river contaminants...

...transcriptional activation screening. Nat Protoc. 2017 (4):828-863. doi: 10.1038/nprot.2017.016. Additional Resources...Cells were cultured following a differentiation protocol before performing single-cell RNA sequencing. ... refer to the Zhang lab’s previously published protocol and its relevant modifications on the pooled library...looking for the best astrocyte differentiation protocol! Ready to try it out for yourself? Check out ...

...CRISPR protein required is the Cas9 endonuclease or a variant thereof. This individual protein has all...RNA provided that target is upstream (5') of a protospacer adjacent motif (PAM) Cas9 endonuclease binding... RNA and a 3-base pair sequence known as the Protospacer Adjacent Motif or PAM. In order for dsDNA to ...HNH-like nuclease domain near the center of the protein. Upon target binding, Cas9 undergoes a conformational...

... GEMCherry2, an improved monomeric fluorescent protein for assessing CRISPR/Cas9 activity Guide RNA structure... labor-intensive, require multiple fluorescent proteins, and aren’t that efficient. To overcome these ...developed a modified monomeric red fluorescent protein (RFP), named GEMCherry2, that can rapidly and efficiently... M (2018) A Modified Monomeric Red Fluorescent Protein Reporter for Assessing CRISPR Activity. Frontiers... CRISPR blog posts Find more about fluorescent proteins Get a refresher on CRISPR with our CRISPR 101 ...

...trying to find a relatively rare protein in an experiment with many proteins of interest! Research the brightness...  However, antibodies conjugated to horseradish protein (HRP), primarily used in Western blots and ELISAs...of the most complex subjects in antibody-based protocols - but thankfully this is an Antibody 101 post,...Try to inversely pair fluorophore strength with protein abundance.    Second, you’ll need to consider...

.... Antibody heavy chain proteins as well as the genes that encode those proteins are designated by the ...antibody isotypes. Binding to complement system proteins, which can initiate a cascade that helps attack... IgG2, IgG3, and IgG4. IgE IgE antibodies help protect against parasites but also play a role in allergies...secretions (e.g. tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly...

...Envelope proteins determine which types of cells a virus can infect. The VSV-G envelope protein enables... the Bob Weinberg lab and contains an envelope protein for producing lentiviral and MuLV retroviral particles...For more information, see the Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more slowly than ...pSpCas9n(BB)-2A-GFP (PX461) expresses both a Cas9 protein and a gRNA. But instead of Cas9, this plasmid expresses...

...binding with Rep proteins and mutations in the trs site reduce Rep nicking. Since Rep proteins are important...with the viral rep (replication) and cap (capsid) proteins, which bind to the ITRs and initiate replication...resolution site that serves as the target site for Rep proteins (Savvy et al. 2013). Figure 2: AAV2 wild type... based vector improvements unrelated to capsid protein modification. Front Med, 10, 1106085. https://doi.org...

...awards to early career scientists using fluorescent proteins in their research. These awards help early career...the development and dissemination of fluorescent protein tools. This year, we’ve made some changes. In addition...thiol-containing acceptors. These hydropersulfides modulate protein activity and have important roles during oxidative...inhibits the neuron (via hM4D). She fused fluorescent protein tags to the receptors to monitor their localization...-expressed with a photoconvertible fluorescent protein mEOS4a. Cells containing a sensor that shows a ...

... elements – promoters, origins of replication, protein tags, and antibiotic resistance markers (just to...as the “cyclization recombinase”), is a 38 kDa protein responsible for intra- and inter-molecular recombination... Cre acts independently of any other accessory proteins or co-factors, thus allowing for broad applications...single loxP site are recognized and bound by a Cre protein, forming a dimer. The two loxP sites then align... a parallel orientation, allowing the four Cre proteins to form a tetramer. A double-strand DNA break ...

...detect, purify, quantify, deplete, and visualize proteins of interest (Greenfield, 2014). Traditionally,...chain for the antibody and when translated into protein will assemble into a fully functional antibody....encoded in a plasmid. (Right) An assembled antibody protein binds an antigen. Image from Fvasconcellos. ...genes for antibody expression. If starting from a protein sample (for instance, affinity purified antibodies...well in animals because of similarities to host proteins. After the antibody of interest has been cloned...