...Passion? A love of learning? No. Or at least not entirely. What truly unites us all is the frustration of... to the day before, which got rid of the issue entirely. In many cases, the answer isn't to work harder...double-checking details, or just taking a moment to relax. During really stressful days, I would even take...

...Cancer and the Immune System: Deciphering the Relationship” by our guest blogger Subhadra Jayaraman, a ...platform focused on analysing immune measures and correlating them with tumor mutations and clinical responses...Cancer and the Immune System: Deciphering the Relationship Resources on Addgene.org Browse Cancer Plasmids...

...protein properties need to be scrutinized under relevant conditions. The properties of fluorescent proteins...several important experimental conditions that are related to the imaging strategy and the sample. Practical...the assay is depicted in figure 1. The strict correlation between the fluorescent protein of interest and...

...is software product management, and how does it relate to science? In the simplest of terms, a software...project management  Bolster your skills in strong relationship-building and influence. You’ll need a large ...large deal of influence and strong relationship-building in order to rally many stakeholders behind a common...

...that contain your plasmid backbone, as it often relies on antibiotic resistance. But what about identifying...method of screening is the blue-white screen, which relies on the lacZ gene. lacZ encodes the enzyme 𝛽-galactosidase... functional 𝛽-galactosidase. Blue-white screens rely on vectors where the gene of interest is inserted...

...mutant and wild type stem cells into any cell type relevant to the disease in question – cardiomyocytes, adipocytes...olden days," not even five years ago, scientists relied on traditional homologous recombination. That technology...will bind desired sequences. TALENs are much more reliable. If you design them for a particular sequence,...

...antibodies to antibody fragments to tools completely unrelated to antibodies.  Why use an alternative affinity...either re-develop or re-optimize your assay for an entirely new affinity reagent.    Fig. 1: A comparison... These are antibody mimics and are not closely related to antibodies. Instead, they are derived from a...

...targeted Tet-on transgene vector. Degron tags Tagging relevant proteins with a degron tag can provide inducible...endogenously or exogenously expressed protein with the relevant tag and then introducing a specific ligand to ...unstable and quickly degraded. However, if the relevant small molecule is introduced into the system, ...

...bidirectional transcription. In an uninfected cell, dsRNA rarely circulates, but upon viral infection, this nucleic...barring a loss of mitochondrial integrity that would release them into the cytosol. Fig. 2: Endogenous...upon viral infection. Endogenously produced dsRNA rarely reach this threshold and thus do not typically ...

...salaries - in some cases reversing these decisions entirely. At Future of Research, we had been collecting...available (below). However, on November 22nd a preliminary injunction against the FLSA updates was granted...say, salaries are showing surprisingly little correlation with cost of living at the location of the institution...

...identifies RNA-protein interactions. Both methods rely on the promiscuous E. coli biotin ligase, BirA*,...that promote RNA decay and their upregulation is correlated with poor survival in breast cancer patients.... wild-type sequences and that loss of binding correlated with increased serum iron levels seen in HHCS...

...future prospects. Among the topics discussed, many related to the structure of the biomedical workforce. Quotes...results, not scientists”  These comments are well correlated with published data. For example, in 2010, less...grants through the Sloan foundation for postdoc-related data collection and analysis. He is also a strong...

...scientists are aware of the benefits of coaching relationships and do not know where to look for a coach. Many...thinking about what your goals are for the coaching relationship. Do you want to change jobs? Grow your professional...the coach to define how you will know that the relationship has been successful – what are the desired outcomes...

...Within the animal kingdom, this information is relayed through factors (both of endocrine and dietary ...systematic effort to compile NR- and coregulator-related global gene profiling datasets in to our data mining...coregulators. We look forward to growing this important relationship with Addgene for the benefit of the entire research...

...   Epigenetics nation stuck with methylation correlation  In the 1980s, a lot of things were cool: Walkmans...problem is that many of these studies report correlations without clearly testing whether a certain methylation... dCas9-TET, allowing one to study the causal relationship between DNA demethylation and gene expression...

...early-phase clinical trials and have shown promising preliminary results (Dimitri et al). CRISPR engineering of...patients experiencing sustained and substantial relief from both disorders. As of publication, submissions...The future success of CRISPR in the clinical will rely on improving and evolving better CRISPR systems ...

..., give it back to you, and for you to make the relevant alterations before submitting - handing them your...in your research proposal are originality and relevance. When reading through your proposal, ask yourself...your work, or even the successful use of your preliminary data to secure further funding. Make it clear...

...fluorophore--think of it as a virtual photon 3. Relaxation of the acceptor fluorophore back to its ground...donor-acceptor pair and a change in FRET efficiency correlates with a change in the distance and/or orientation...simultaneously would further increase the amount of correlated information available from a cellular process...

...terminals (Lafon 2005). Therefore, once RABV is released from an infected cell, it is only taken up by ...genome). Nascent G protein-coated RABV is then released from the starter cell and transmits retrogradely...mediated by other viral proteins), but the newly released RABV cannot infect any subsequent cells due to...

... HR carefully because sometimes companies have relaxed dress codes for interviews. You can also ask HR... know up front how you plan to take questions.  Relax!  You wouldn’t have made it to the interview if ...position. So prepare for the interview, but then relax.  Additional resources on the Addgene blog: Tips...