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We narrowed to 602 results for: she

Showing: 341 - 360 of 602 results

  1. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...and the replication control machinery (Need a refresher on the Origin of Replication? See our Plasmids....2015.03.007 Pubmed PMID: 25889267 Ebersbach G, Sherratt DJ, Gerdes K (2005) Partition-associated incompatibility...

  2. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...combined all the domains into a single protein, shedding the complexity of its ancestors. They classified...CRISPR 101: RNA Editing With Cas13  CRISPR Cheat Sheet Resources at Addgene.org CRISPR Guide...

  3. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...For more details, check out this review article published in Reviews in Medical Virology (Li et a., 2018...doi.org/10.2174/1566523054546224 DePolo NJ, Reed JD, Sheridan PL, Townsend K, Sauter SL, Jolly DJ, Dubensky ...

  4. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...strategy Figure 4 shows a gating strategy from a published experiment (Barlow-Anacker et al., 2017). This...Neonatal immune response to rhinovirus A16 has diminished dendritic cell function and increased B cell ...

  5. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...Small’ data produced by the research group in spreadsheets, Word docs, and the like is likely stored in... documents, but also to the associated data (spreadsheets, images, sequencing data, etc.) used and/or ...

  6. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...continual production of MazE, the toxin becomes active. Shen Yang’s lab made use of mazF as a counterselectable...PMID: 12718874. Yang, Junjie, Weihong Jiang, and Sheng Yang. "mazF as a counter-selectable marker for unmarked...

  9. Optogenetics Guide

    Type
    Guide
    ... Prigge M, Schneider F, Davidson TJ, O'Shea DJ, Sohal VS, Goshen I, Finkelstein J, Paz JT, et al. 2011...Ramakrishnan C, Mattis J, Prakash R, Diester I, Goshen I, Thompson KR, Deisseroth K. 2010 Molecular and... Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R, Gunaydin LA, Hyun M, Fenno LE, Gradinaru...

  10. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... you're new to viral vectors or simply need a refresher, we've included a glossary at the end! Browse ...vectors, they offer the benefit of having well-established protocols for fast and efficient generation of...large-scale, high-titer preps. This is thanks to established retroviral production cell lines that are able...Retroviruses . Cold Spring Harbor Laboratory Press. NIH Bookshelf De Ravin, S. S., Su, L., Theobald, N., Choi, U...

  11. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ... (Wu et al., 2004). Over time, the expression diminishes as the episomes are lost through cycles of cell...K., Schmidt, M., von Kalle, C., Gaspar, H. B., Thrasher, A. J. (2008). Insertional mutagenesis combined...

  12. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...have a good mixture of useful tasks that can be accomplished quickly and things that will take longer. Completing...Throughout the day, I cross items off as they are accomplished or I add to the list as the need arises.Why ...

  13. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the... including the development of crops with longer shelf life and the development of disease-resistant crops...

  14. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...offended. I felt terrible when the host told me and wished I had been more careful. 3. Tell a story We are...thinking “What happens next?”  Describe how you pushed to a solution or overcame obstacles. Take time ...

  15. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...enough T-cells could be reached to ablate a well-established viral reservoir. A second challenge for CRISPR...developing resistance. Shortly after Kaminski et al. published their results, Wang et al. showed that HIV-1 could...

  16. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ... treat disease postnatally. Now, three papers published concurrently in Science have shown CRISPR can ...McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton JM, Bassel-Duby R, Olson EN. Postnatal genome editing...

  17. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...read a paper and saw an interesting construct you wished to use, you could either make it yourself or you...Intracellular Calcium Sensor Yang, J. et al. (7) wished to trigger intracellular Ca++ release with an optogenetic...

  18. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...  Once you have chosen an antibody read the data sheet thoroughly. Vendors will frequently include suggestions...proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of...

  19. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... Drosophila cells. Work by Wei et al. from Kang Shen’s lab has also shown that the Q system is also functional...Wei, Xing, Christopher J. Potter, Liqin Luo & Kang Shen. “Controlling gene expression with the Q repressible...

  20. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...enhanced random integration efficiencies that are accomplished with transfections and are therefore virus-free...enhance gene editing? Gene editing can now be accomplished in a more cost-effective and efficient manner...
Showing: 341 - 360 of 602 results