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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 537 results for: try

Showing: 341 - 360 of 537 results

  1. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ... like serotype, promoter, or tool type and each entry contains an image along with key experimental details...to input your data for sharing on the site. The entry form has 5 general question types and it takes approximately...use GCaMP expression? Direct Fluorescence? Immunohistochemistry? Did you use any relevant animal treatment...

  2. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...student hasn’t been asked the question: “Academia or industry?” Once academia was the clear answer with a well-defined...uses it to match qualified scientists to jobs in industry, academia, and government. This great resource... more information Follow people in academia, industry, and government on social media (Twitter, Facebook...

  3. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...resulting in faint signals (Schwab et al., 1979; Buttry & Goshgarian, 2015). Nevertheless, the use of these...visualizing motor, visual, and auditory neuronal circuitry in vivo. A variant of RABV has been developed ... allowed for detailed mapping of the neuronal circuitry involved in learning and memory (Kohara et al....PMID: 21825165. PubMed Central PMCID: PMC3174680. Buttry, J.L., and Goshgarian, H.G. (2015). WGA-Alexa Transsynaptic...

  4. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...unique regions as well, though. Viral packaging and entry The genetic cargo requires viral packaging to infiltrate... from the transfer plasmid. Viral packaging and entry The viral packaging proteins for AAV are rep and...replication of the dsDNA virus. Viral packaging and entry Adenovirus is quite large and has more helper plasmid...

  5. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...diverse array of processes. The food processing industry, for instance, employs psychrophilic enzymes for...extremely useful benefits for any food processing industry as it can potentially help minimize the incidental...iGEM teams to interact with personnel from the industry and brainstorm with each other. The event also...

  6. Antibodies 101: Isotypes

    Type
    Blog Post
    ...applications including flow cytometry, immunohistochemistry, immunocytochemistry, ELISAs, and Western blotting...

  7. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...industries tend to hire their former interns to fill entry-level positions right out of college. Prior to March...also serve you well when you are applying for an entry-level position following graduation. While these...for internships, scholarships, fellowships, and entry-level positions post graduation. Many thanks to...

  8. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...visualization of specific proteins in situ immunocytochemistry is perhaps the most common approach. With...students at the Max Planck Institute for Biophysical Chemistry, Göttingen in Germany. I attended this symposium... labeled proteins can be detected using click-chemistry that covalently links the azido containing AHA...

  10. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ... of transitioning a scientist from academia to industry, you might want to consult the Cheeky Scientist...We Turn Academics into Confident and Successful Industry Professionals”. This larger shop might appeal ...excellent in coaching for communication skills. Industry transition is also an area of specialty for Michelle...

  11. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team from China’... (i.e. RNA replication with no proteins). The chemistry is complex and this work requires an interdisciplinary...forms as we shared millions of years of common ancestry with worms before evolutionary divergence. Nikolaus...

  12. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...challenging to compete in today’s tough academic or industry job market for biology PhD’s. I was facing the...in 2007. Nikolai trained as a post-doc at the Biochemistry Department at the University of Manchester as...receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in...

  13. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...human genes and are recommended for use in immunohistochemistry (Figure 5). Anti-MAPT [AT8] specifically...Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant..., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org...

  14. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...can be easily modified in order to facilitate its entry into the target cells (Beatty et al., 2012). These...Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far) or for cancer...therapy: Current state and clinical perspectives. Biochemistry Moscow 81:700–708 . https://doi.org/10.1134/...

  15. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...machine learning to solve difficult problems in chemistry and chemical biology.       David R. Liu is Director... Faculty at the Broad Institute; Professor of Chemistry and Chemical Biology at Harvard University; and...Institute Investigator. Liu’s research integrates chemistry and evolution to illuminate biology and enable...

  16. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...generates pores in the cell membrane, allowing for entry of the RNP into the cytoplasm. In addition to the...forms pores in the nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated...electroporation of zygotes." Journal of Biological Chemistry (2016): jbc-M116. PubMed PMID: 27151215. PubMed...

  17. Hot Plasmids - November 2023

    Type
    Blog Post
    ...label proteins with an alkyne group for click chemistry reactions instead of biotin — a valuable development..., then the other, a dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels...their initial tests with thick slice (70 μm) immunohistochemistry (IHC) (Figure 5). PSD-95 is a scaffolding...

  18. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...those conversations, reading online healthcare industry publications like FiercePharma, FierceBiotech,...about the trends and workings of the healthcare industry. Finally, on-campus resources like career offices... both provide up-to-date information about the industry, recruiting processes, and can connect you to ...

  19. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... chemically pre-treating the cells to allow for entry of the antibodies. Here, a two-step process is employed.... The main applications of dye stains in flow cytometry are cell proliferation and cell viability. Unlike...101: Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...

  20. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... unconjugated and recommended for use in flow cytometry. Based on the established applications of the ...will also work in immunoprecipitation and immunohistochemistry. Table 1: IPI antibodies  Antibody ...Addgene's Antibody Guide Antibodies 101: Flow Cytometry Antibodies 101: Introduction to Antibodies...
Showing: 341 - 360 of 537 results