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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 399 results for: MUT

Showing: 361 - 380 of 399 results

  1. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...with the pooled library to create a population of mutant cells that are then screened for a phenotype of...Human Wei 3rd Varies 12,472 pairs HR700_TP53 Exon Mutation Libraries 229137–229140 Donor Vector Human Stiewe...

  3. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...sgUPRT 54467 Other/Toxoplasma gondii none, Q5 mutagenesis yes, cut S. pyogenes Sibley pU6-Universal 52694...Gateway recombination. Qi Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs...

  4. Immunology Research Plasmids and Resources

    Type
    Collection
    ...arginine-rich, mutated in early stage tumors ARP, MANF, MGC142148, MGC142150 ARMETL1 arginine-rich, mutated in early..., DC-SIGN, DC-SIGN1, MGC129965 CD4 CD4 molecule CD4mut CD74 CD74 molecule, major histocompatibility complex...FLJ79544, FLJ94613, MGC138597, T3G CD4 CD4 molecule CD4mut CD40LG CD40 ligand CD154, CD40L, HIGM1, IGM, IMD3...consists of 182 wild-type and 19 well-characterized mutant ORFs that represent over 25 cellular signaling ...

  5. CRISPR Guide

    Type
    Guide
    ...developed by mutating specific amino acid residues to reduce off-target editing. Some mutations disrupt interactions...off-target mutations in DNA, RNA, or both, and are recommended in contexts where such mutations would be... and a gRNA will result in a diverse array of mutations (see: Validate genetic modification ). In most...amino acid deletions, insertions, or frameshift mutations leading to premature stop codons within the open...targeted gene. The ideal result is a loss-of-function mutation within the targeted gene. However, the strength...strength of the knockout phenotype for a given mutant cell must be validated experimentally. Browse Plasmids...(nick) or no cuts. Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain and one...

  6. Browse

    Type
    Collection
    ...Plasmids Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry PI...

  13. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...making it a very flexible editing system. The T375G mutation reduces off-target effects of ADAR2 by destabilizing...

  14. Optogenetics Guide

    Type
    Guide
    ...improve these natural opsins - by inducing point mutations to alter the absorption spectrum or adding trafficking... variants have been created via genetic point mutations, codon optimization, and chimeric fusion of domains...photocurrent compared to CrChR2 450 ChETA E123T mutation; creates faster kinetics but reduces photocurrent...photocurrent amplitude 490 ChR/T159C T159C mutation; displays increased photocurrents 470 SFO/SSFO Step function...function Opsins and Stabilized Step Function Opsins. Mutations at C128 and D156 of CrChR delay the closing of...Chlamydomonas noctigama . ChrimsonR carries K176R mutation. 590 PsChR2 High-efficiency blue-shifted channelrhodopsin... C1V1 ChR1-VChR1 chimera with E122T and E162T mutations 540 Channelrhodopsins: chloride channels iChloC...

  15. Resolute Plasmid Collection

    Type
    Collection
    ...approximately 450 identified members. Many SLCs have mutations associated with disease and could become targets...

  16. Mammalian RNAi Tools

    Type
    Collection
    ...or to knockdown genes with potentially harmful mutations in therapeutic settings. As a tool in mammalian...

  17. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...modification, or variant. ID Plasmid Description Mutations Industry PI Due to export control regulations,...

  18. CRISPR Plasmids and Resources

    Type
    Collection
    ...enzymes generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand DNA ...

  19. Plan Your Experiment

    Type
    Guide
    ...knockout )? Generate a specific mutant allele of a gene ( point mutant )? Increase or decrease expression...Furthermore, in cells containing two mutated alleles, each mutated allele may be different owing to the... type or organism without a specific preferred mutation Cas9 (or Cas9 nickase) Single (or dual) gRNA targeting... a particular gene, such as generating a point mutation or inserting a tag Cas9 (or Cas9 nickase); Base...knockout. Base editors can make a limited set of mutations. Repress or Interfere (CRISPRi) Reduce expression... the N-terminus are targeted since frameshift mutations here increase the likelihood that a nonfunctional...of NHEJ. In HDR gene editing experiments, most mutated alleles will not contain the desired edit, as a...

  20. Adenovirus Plasmids

    Type
    Collection
    ...modular inserts for seamless assembly of multiple mutations, alterations, or transgene insertions into single...
Showing: 361 - 380 of 399 results