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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 916 results for: Tro;

Showing: 901 - 916 of 916 results

  1. Protocol - How to Streak a Plate

    Type
    Protocol
    ...Recovering Plasmid DNA from Bacterial Culture Introduction If you have a glycerol stock or stab culture...would hold a pencil, so that you can make a broad stroke. Only touch the surface of the plate, do NOT dig...

  2. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...Recovering Plasmid DNA from Bacterial Culture Introduction This protocol describes methodology for plating...concentrations of carbenicillin plated over-agar. Control Plate with No Carbenicillin Plate shows a lawn ...

  3. Weighing Reagents Protocol

    Type
    Protocol
    ...Protocols Weighing Reagents Weighing Reagents Protocol Intro to the Lab Bench Check out more protocols and videos...videos to help you get started in the lab! Introduction For many experiments, you’ll need to make buffers...

  4. Water Bath Protocol

    Type
    Protocol
    ... Protocols Water Bath Water Bath Protocol Intro to the Lab Bench Check out more protocols and videos...videos to help you get started in the lab! Introduction A water bath is a piece of laboratory equipment...

  5. General Transfection

    Type
    Protocol
    ...Virus Blog Posts Mol Bio Protocols Viral Service Introduction This protocol describes a general method for...channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents DMEM high ...

  6. Pipetting Protocol

    Type
    Protocol
    ... Protocols Pipetting Pipetting Protocol Intro to the Lab Bench Check out more protocols and videos...videos to help you get started in the lab! Introduction The pipette is an essential tool for nearly anyone...

  7. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...Vector Guides Virus Blog Posts Mol Bio Protocols Introduction This protocol can be used to purify AAV of any...VP2, VP3; M protein marker. Image adapted from Strobel Benjamin, Miller Felix D., Rist Wolfgang, and Lamla...

  8. AAV ddPCR Titration

    Type
    Protocol
    ...Virus Blog Posts Molecular Biology Reference Introduction This protocol describes how to use droplet digital... and positive droplets (blue). The no template control (NTC) should be close to zero (B08). At Addgene...

  9. AAV Production in HEK293 Cells

    Type
    Protocol
    ...Virus Blog Posts Molecular Biology Reference Introduction This protocol can be used to produce AAV from... cell counter Ice bucket CO 2 incubator Pipet controller Hazardous waste container pH meter Stir plate...

  10. Cloning

    Type
    Guide
    ...principle to Gibson cloning, but instead of an in vitro reaction with purified enzymes, it takes advantage... manner. To accomplish this, you just need to introduce into the yeast the two (or more) fragments of ...

  11. Protocol - How to Design Primers

    Type
    Protocol
    ...Reaction Plasmid Cloning by PCR Agarose Gel Electrophoresis Primer Design for PCR Oligonucleotide primers...

  15. Educational Resources

    Type
    Guide
    ...plasmid cloning, and virus, including: Gel Electrophoresis Bacterial Transformation Plasmid Cloning Creating...

  16. Pouring LB Agar Plates

    Type
    Protocol
    ...Blog Posts Mol Bio Protocols Cloning Protocols Introduction Plasmids can carry one or more antibiotic resistance...
Showing: 901 - 916 of 916 results