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We narrowed to 964 results for: app

Showing: 901 - 950 of 964 results

  11. Common Injection Routes in Mice

    Type
    Blog Post
    ...expertise available in your lab or organization, and approvals from the organization overseeing your work. Good...

  19. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b truncations...

  20. DNA Purification Without a Kit

    Type
    Blog Post
    ...rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the glass particles in ...

  23. Plasmids 101: Protein tags

    Type
    Blog Post
    ...deciding on which one to use will depend on your application (see table below). The antibodies available for...

  40. Fluorescence Titering Assay

    Type
    Protocol
    ... these cells can still be used in downstream applications. Safety Warnings Lentivirus is generally considered...

  41. General Transfection

    Type
    Protocol
    ...), but any HEK293T cell line should work. This approach can be adapted for different cell lines and different...

  47. AAV Production in HEK293 Cells

    Type
    Protocol
    ...incubator for ~72 h. Harvest cells and media by tapping the sides of the CS5. Cells should detach easily...

  48. Antibody Guide

    Type
    Guide
    ...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...of signaling molecules. Select one appropriate for your application and experimental design. Will you ...transcriptomics or proteomics. This approach is often appropriate for quantitative methods. Capture MS...protein of interest, with no overlapping epitopes. It is not appropriate for validation of an antibody...production and storage techniques, and explain common applications. Science... Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods...

  49. CRISPR Guide

    Type
    Guide
    ... (2015). A scalable Genome-Editing-Based approach for mapping multiprotein complexes in human cells. Cell...technologies. Before CRISPR, genome engineering approaches like zinc finger nucleases (ZFNs) or transcription-activator-like...rapidly became the most popular genome engineering approach. Figure 1: Overview of the basic CRISPR mechanism... to various Cas enzymes have extended CRISPR applications to increasingly complex functions, including...understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR... Cas12a, which are available for specialized applications. Check out our list of additional Cas proteins...genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using...

  50. Guide to Using Pooled Libraries

    Type
    Guide
    ...library. Apply pooled library to cells. DIVERGE: Positive and negative screens POSITIVE screen: Apply library...control sample (no selection) In parallel, apply library Apply selection; most cells will survive Perform..., semi-random sequences that can be used for applications like lineage tracing or parsing the effects ...library Apply selection Most cells die or do not pass selection (in the case of a reporter) Sort “winning...genes involved in the selection NEGATIVE screen: Apply library Perform Next Generation Sequencing (NGS)...and control cells Generate a list of gRNAs that disappear with addition of the selection mechanism Positive...
Showing: 901 - 950 of 964 results