We narrowed to 444 results for: ENA

1. Tips for Using BLAST to Verify Plasmids

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   Blog Post

   ...program uses a smaller word size and lower scoring penalties for mismatches and gaps in the alignment. If you...

2. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   ...et al. 2020); however, the technology potential enabled by CRISPR-transposons has yet to be fully realized...

3. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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   ...chemistry and evolution to illuminate biology and enable next-generation therapeutics. Prime editing, base...

4. Simplify Cloning with in vivo Assembly

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   ...beta-actin promoter of the pHLsec vector. In this scenario, IVA can be employed by cutting the vector with...

5. Summer SciComm Series: Modes of Communication

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   ...come to you (a TikTok scicomm account aimed at octogenarians is going to be a hard sell!) Digital platforms...

6. Droplet Digital PCR for AAV Quantitation

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   Blog Post

   ...Increasing the elongation time to 2 minutes and the denaturation time to 1 minute has been shown to increase ...

7. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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   Blog Post

   ...substitute for sound techniques, routine machine maintenance, and critical interpretation of the results.  ...

8. AAVs CREATed for Gene Delivery to the CNS and PNS

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   ...making pretty pictures (see figure 4). It also enables single-cell anatomical study and neuronal tracing...

9. MXS Chaining

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   Blog Post

   ...directed localization of the fluorophore, and thus enabled labeling of particular structures (Table 1). Each...

10. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post

    ...of green fluorescent protein (GFP) sparked a renaissance in biological imaging. Suddenly, cell biologists...

11. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post

    ...two halves of the luciferase protein interact and enable intein splicing (5). Screening with yeast two hybrid...

12. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ...-toxic and could be expressed in living cells, enabling the study of dynamic, physiological processes....

13. Negotiating Work and Life: How to Find the Joy

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    Blog Post

    ...home no matter how busy I am. Believe it or not, teenagers just go to bed and you’ll never know it so don...

14. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    Blog Post

    ...FLP-FRT, Cre-lox, and how combinations of FLP and Cre enable additional levels of genetic control. What is FLP...

15. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    ...PMC5424143. 2. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling of off-target cleavage by...

16. Analyzing CRISPR Editing Results with ICE from Synthego

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    Blog Post

    ...quantitative, NGS-quality analysis of CRISPR editing - enabling a ~100-fold reduction in cost relative to NGS-...

17. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post

    ...be used as a readout for associated emotional phenomena like stress levels, but researchers have to be...

18. Save Time with Transient Plant Leaf Transformations

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    Blog Post

    ...cultures or plants, our transient expression method enables us to quickly screen and investigate transcription...

19. CRISPR-mediated Plant Base Editors

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    Blog Post

    ...gene system to clone multiple protospacers which enables us to target more than one genomic site simultaneously...

20. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post

    ...restriction-modification systems mediate plasmid maintenance." Journal of bacteriology 177.12 (1995): 3451...