... Bacterial Culture Introduction This protocol describes methodology for plating antibiotic over-agar for...

...hot plasmids segment on the Addgene Podcast. Subscribe to the Addgene blog to get all the latest hot ...

...Protocols Viral Service Introduction This protocol describes a general method for transfecting mammalian cells...

...gene of interest in cell culture. This protocol describes how to transfect suspension HEK293 cells with ...

... several μg/μL. The protocol below is meant to describe the general procedure for purifying plasmid DNA...

...from Feng Zhang (Addgene plasmid #52962 ) and is described in Improved vectors and genome-wide libraries ...

...plasmid of interest. For this example, we will describe how to copy a cDNA from one vector into a new ...

...Purification Protocol Introduction This protocol describes how to determine the purity and concentration ...

... Biology Reference Introduction This protocol describes how to use droplet digital PCR (ddPCR) to titer...

... Biology Reference Introduction This protocol describes how to extract RNA from tissues or cells without...

...Guide Virus Blog Posts Introduction This protocol describes how to use droplet digital PCR (ddPCR) to titer...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

...license or rights of access or use to the materials described in the literature. The distribution of materials...

...RNA polymerases that all transcribe different genes. RNA polymerase I transcribes genes encoding ribosomal...binding of RNA polymerase to DNA. RNA polymerase transcribes DNA to mRNA which is ultimately translated into...upstream (5’) of the sense or coding strand of the transcribed gene. The coding strand is the DNA strand that...-coding strand as this is the strand that is transcribed by the RNA polymerase. DNA sequences called response...proximal promoter, and distal promoter. Below describes the specifics of these regions in eukaryotic cells... can bind, and the structural genes that are transcribed together. Operon regulation can be either negative... in the opposite way. The operon is normally transcribed until a repressor/corepressor binds to the operator...

... is described in more detail in the Multiplex Assays section below. The following table describes the ...of B cells isolated from animals immunized as described above are used to create hybridomas, which produce...primary antibody for your protein of interest, as described in the IP section above. Reverse the crosslink...

...lentiviruses most often integrate into actively transcribed genes, and that this preference is conserved ...does not explain the lentiviral preference for transcribed genes. Studies comparing the lentivirus HIV and...

...cell membrane. Microbial opsins, such as those described below, can be targeted and expressed in specific...dimerization. In addition to the applications described above, both LOV and Dronpa can control activity...

...Collection of guides that describe how popular scientific techniques work...

...lentiviral packaging methods. The three components described above (envelope, packaging, and transfer) are ...

...complexes Purified Cas9 protein and in vitro transcribed gRNA are combined to form a Cas9-gRNA complex...