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We narrowed to 416 results for: crispr plasmids

Showing: 361 - 380 of 416 results
  1. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    ...instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction...revolution in genome editing methods (most notably CRISPR/Cas mediated HDR) enables the tagging of endogenous...advisable to first express the fusion protein from a plasmid-based system to assess whether the chimera is still...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...protein fusions Browse the fluorescent proteins plasmid kits ...measures homodimerization of FPs targeted to the endoplasmid reticulum based on the appearance of OSER whorls...
  2. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    Blog Post
    ...research? Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0...1.0, 1.1, 2.1 plasmids Brainbow 3.0, 3.1, 3.2 plasmids References: Transgenic strategies for combinatorial...CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation... of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick primer on how...
  3. How to Deposit Your Plasmids with Addgene

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    ...Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that will not be published are...and entering a plasmid name,plasmid type, and plasmid description/experimental purpose (right). On the...with your plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given...easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with...Spreadsheet". Start your plasmid deposit There are two options to begin your plasmid deposit. Figure 2: Choose... Choose to submit plasmid(s) online or by using a spreadsheet. The plasmid information can be entered ...than 10 plasmids but we recommend using the spreadsheet option for deposits of 10 or more plasmids.   ...
  4. iPSC Neurodegenerative Disease Initiative Plasmid Collection

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    ...Collection You May Also Like... CRISPR Guide CRISPR Protein Tagging Plasmids for Stem Cell Research Neurodegeneration...phenotyping, and distribution efforts. Plasmids The following plasmids can be used to create cell lines with...Find plasmids generated by the iPSC Neurodegenerative Disease Initiative (iNDI) here. Depositor... with the research community. To this end, all plasmids that result from the project efforts will be made...made available for research purposes in the iNDI plasmid collection at Addgene. Figure 1: Schematic of iNDI... cell types, as noted in the purpose field: ID Plasmid Description Additional Resources iNDI (Link opens...
  5. Fluorescent Proteins 101: Aptamer Fluorophores

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    ...Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent... References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc...
  6. Distribution to Industry

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    ...collection of plasmids that are available to industry. Learn more about the available plasmids and how to...Distribution to Industry Featured Collections Search Plasmids Plasmids Kits Pooled Libraries Antibodies Ordering ...type, species, and more. Plasmids Browse the table below to see all of the plasmids that are currently available...Mutation Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation...Fusions from Promega COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson...Biolabs Cancer Research from Sinai Health System Plasmids from the Structural Genomics Consortium Solute...Foundation Search Use Addgene's Search to find plasmids available to industry scientists while also adding...
  7. Viral Vectors 101: An Introduction to AAV

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    Blog Post
    ...transient gene expression in a particular cell type to CRISPR genome editing, optogenetics, and chemogenetics...of both polarities are rescued from the vector plasmid and individually packaged into the AAV capsids....
  8. Allen Institute for Cell Science Plasmid Collection

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    ...Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid Collection You May Also...Also Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization Plasmids...full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited...distribution to the research community. With these plasmids researchers will be able to generate fluorescently...Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells Find additional cell...
  9. Neurodegeneration Research Collection

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    ...Localization , Optogenetics Plasmids , and Chemogenetics Plasmids . Find plasmids for endogenous gene tagging... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...His tags. (Canman et al. Science. 1998) CRISPR Tools Find CRISPR pooled libraries available from Addgene...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other...about the blood brain barrier integrity, and using CRISPR screens to understand more globally how neurons...
  10. Cre-Lox and Other Site-Specific Recombinases

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    ...Site-Specific Recombinases Cre Plasmids Flp Plasmids Dre Plasmids Other Recombinase Plasmids Recombinase-Dependent...Tumor Suppressor Gene CRISPR Knockout Library CRISPR Sidi Chen Use this AAV CRISPR knockout library expressing...Collection Optogenetics Plasmid Collection Monosynaptic Neuronal Tracing CRISPR Plasmids and Resources Content... vectors. Highlighted Plasmids Browse the tables below to find popular plasmids expressing your favorite...tamoxifen”, or “light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional... with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout... Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources ...
  11. Evolution of Lab Techniques

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    ... instance, the popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic... on Analyzing DNA Sequencing Results Check out Plasmid Cloning by PCR Resources on Addgene.org Check...Pages Find Molecular Biology Tools Analyze Your Plasmids by Restriction Digest ...
  12. Viral Vectors 101: The Retroviral Lifecycle

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    ...Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide References Cullen, B. R. (...Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses, 13...
  13. The Challenges of Cell Culture

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    ...Blog Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian Cells Learn about...Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ...
  14. Plasmids for Stem Cell Research

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    ... Plasmid Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research...Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells...utilize plasmids to increase expression of key factors. The tables below will help you find plasmids available...information page. Reprogramming Plasmids Browse the table below for plasmids used to create induced pluripotent...RNA p53 for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive...Differentiation and Transdifferentiation Plasmids Browse the table below for plasmids used for differentiation of ...Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms ...
  15. Antibody Plasmid Collection

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    ...Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant... table highlights plasmid collections that can be used to create and express plasmid-based antibodies....Apr 8. Markus Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies...) ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions for other plasmids that ...collection of plasmid-based antibodies, including monoclonal antibodies, scFvs, and nanobodies. Plasmid... Plasmid Collections...recombinant antibodies made from plasmids in our collection! These monoclonal antibodies are produced in-...
  16. Getting the Most from Your Lentiviral Transduction

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    ...after transfecting with the transfer and packaging plasmids. A benefit of the multiple harvest strategy is...Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources on Addgene.org...
Showing: 361 - 380 of 416 results