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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 539 results for: genome

Showing: 361 - 380 of 539 results

  1. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...openness” of the lab (i.e projects like the Personal Genome Project and open source software, hardware, wetware...

  2. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...CRISPR Purify CRISPR: Purify Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick... Posts Identifying molecules associated with a genomic region of interest in vivo is essential to understanding...immunoprecipitation (ChIP) to allow purification of any genomic sequence specified by a particular gRNA. In the...catalytically inactive dCas9 is used to purify genomic DNA bound by the gRNA. An epitope tag(s) can be...epitope tag. Compared to conventional methods for genomic purification, CRISPR-based purification methods... identification of molecules associated with a genomic region of interest in vivo. For CRISPR-based purification...tables below for CRISPR plasmids to help purify genomic loci. Plasmids are available for expression in ...

  3. CRISPR References and Information

    Type
    Collection
    ...off-targets, target's genomic location, and genome annotation. Available genomes are human (hg19 & hg38...design tool for genome editing, as well as gene regulation (repression and activation). Genome support for...-use computational pipeline for the analysis of genome editing experiments. It enables rapid and intuitive... a new window) . MAGeCK Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK). This tool can...selected sgRNAs, genes, or pathways. For use with genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology...prediction. Developed by the Hyongbum Kim Lab . GenET (Genome Editing Toolkit) (Link opens in a new window) A...well as predict off-targets, for CRISPR in 180+ genomes. Developed by the Jean-Paul Concordet Lab . For...

  4. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...Cas9 is becoming an ever more ubiquitous tool for genome engineering, and studying its structure can help.... Structural biology of CRISPR–Cas immunity and genome editing enzymes. Nature Reviews Microbiology, 20...

  5. Zhang Lab CRISPR Page

    Type
    Collection
    ...are below. Pooled genome-wide human SAM library SAM can be combined with a human genome-wide library to ...vectors for genome editing in the brain Using Cas9 mice Platt et al. demonstrated in vivo genome editing ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...the type II CRISPR nuclease system to facilitate genome editing in mammalian cells (Cong et al ., Science...libraries (both human and mouse) for performing genome-wide knockout screens. The libraries are available...Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome Editing The Zhang Lab has both single- and dual-vector...top AAV - Cas9 mouse CRISPR-Cas9 is a versatile genome editing technology for studying the function of...

  6. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...123,411 Human genome-wide library v1 69763 Knockout Human Wu 3rd 4 77,406 Human improved genome-wide library...Mouse genome-wide library v1 Discontinued Knockout Mouse Yusa 3rd 5 87,897 Mouse improved genome-wide ...Pooled Libraries Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...more complex than using CRISPR to modify a single genomic locus. Before ordering, please review the steps... - A given CRISPR library may target the entire genome or a specific class of genes (subpools). Species...within a given CRISPR library are unique to the genome of particular organism, and the library is only...Herpes Virus (KSHV) Cow ( B. taurus ) Library Size Genome-wide Subpool Viral Prep Available Yes No Clear ...

  7. Plan Your Experiment

    Type
    Collection
    ...sequence and your genomic target may reduce the gRNA activity. Be sure to sequence the genomic region you intend...system that enables researchers to manipulate the genome with relative ease. Over the past decade, researchers...Homology Directed Repair (HDR) Generate specific genomic edits, like point mutations or small tag insertions...gene expression without permanently modifying the genome sequence dCas9 or dCas9 fusion with transcriptional...gene expression without permanently modifying the genome sequence dCas9 fusion with transcriptional activator... chosen Cas enzyme at the right spot within the genome relies on proper gRNA design. While there are some...sequence with no homologous sites elsewhere in the genome. In reality, a given gRNA target sequence will ...

  15. Clear and Accessible Writing

    Type
    Blog Post
    ...are captured at the 17%, 25%, and 60% layers, and genome-containing virions are captured at the 40% layer...

  16. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...Protein-DNA Interactions CRISPRainbow and Genome Visualization CRISPR Between the Genes: How to Experiment...methods and tools for studying chromatin and the epigenome. If you have been considering some new techniques...Regulation CRISPR 101: Epigenetics and Editing the Epigenome CUT&RUN: An Improved Method for Studying Protein-DNA...Experiment with Enhancers and Epigenomics I would like to thank the many scientists with me at the Keystone ...

  20. Working with Nuclear Receptors

    Type
    Blog Post
    ...receptors and signaling cascades conceivably allows the genome to sense the impact of the total environment on...
Showing: 361 - 380 of 539 results