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We narrowed to 528 results for: genome

Showing: 361 - 380 of 528 results
  1. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ... plasmids for CRISPR-Cas and Cascade for large genomic deletions....into a DNA “shredder” system that can cause large genomic deletions. Compared to deletions generated by Cas9...
  2. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...multiple components) to target locations in the genome defined by a guide RNA. The transposase can then...insertions. CASTs are most often used in bacteria genome editing, though some mammalian systems have been...
  3. TALEN Engineering

    Type
    Collection
    ... Genome Engineering TALEN Kits TALengineering Reagents ...TALengineering website. Users may also wish to join the Genome Engineering newsgroup. An upgraded version of the...REAL Assembly TALEN Kit: TALE-LSD1 plasmids for epigenome editing...
  4. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...flanking regions comprised of upstream and downstream genomic sequences (Figure 2B).   Figure 2: A comparison... boxes. Highlighted blue regions show flanking genomic sequence. Created on BioRender.com. When it...
  5. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...sequence, such as the Human papilloma virus (HPV) genome, via a crRNA. An ssDNA-fluorescently quenched (...Learn more about Cas12a (Cpf1) and its multiplex genome editing abilities Resources on Addgene.org Checkout...
  6. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ... Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn about Science Communication using...
  7. AAV Molecular Tools

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    Collection
    ...Affinity Purification Neurophysiology Cell Ablation Genome Engineering Labeling Overexpression Tracers Tetracycline...reliant on Flp recombinase. 5 Kazunari Miyamichi Genome Engineering Tools These AAV encode Cas9 nuclease...nuclease used in the CRISPR-Cas9 system for targeted genome editing. ID Name Expression System Activity Serotype...
  8. Editor's Choice, August 2016

    Type
    Blog Post
    ...technology, researchers are having trouble getting NgAgo genome editing up and running in their labs. Pooran surveyed... Genes: How to Experiment with Enhancers and Epigenomics 5 Tips for Troubleshooting Viral Transductions...
  9. Synthetic Biology - Assembly Standards Guide

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    Collection
    ...Browse All SynBio Assembly Standards SynBio Blog Genome Engineering A distinguishing feature of synthetic...Allows for in-frame fusions; NheI is rare in E. Coli genome. Prohibited Restriction Sites: EcoRI, SpeI, NheI...
  10. Build Your CRISPR Vocabulary

    Type
    Blog Post
    .... Structural biology of CRISPR–Cas immunity and genome editing enzymes. Nature Reviews Microbiology, 20...
  11. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...cytosine base editor, BE3, induces a low frequency of genome-wide off-target DNA editing. Using several rapid...
  12. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... prime editor plasmids for (search and replace) genome editing....designed with a spacer that binds to a specific genomic DNA locus and directs the nickase to the target...
Showing: 361 - 380 of 528 results