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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 535 results for: genome

Showing: 361 - 380 of 535 results

  3. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...openness” of the lab (i.e projects like the Personal Genome Project and open source software, hardware, wetware...

  4. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...References & Information Genomic Deletions in Mammalian Cell Lines CRISPR: Protocol for Genomic Deletions in Mammalian...eukaryotic genome engineering. CRISPR/Cas9 is an inexpensive, facile, and efficient genome editing tool...Journal of Visualized Experiments (JoVE) about genomic deletions in mammalian cell lines using CRISPR/... labs details the use of CRISPR/Cas9 to create genomic deletions in mammalian cell lines. Along with the...publication here . Article Citation Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9...design, cloning, and delivery for the production of genomic deletions. In addition, we describe techniques ...identify guide sequences that minimize identical genomic matches or near-matches to reduce risk of cleavage...

  5. The SGC Oxford Collection

    Type
    Collection
    ...Oxford Collection Structural Genomics Consortium Plasmids Structural Genomics Consortium Plasmids - Oxford...Structural Genomics Consortium (SGC) Oxford collection Plasmid... Plasmid Collections Structural Genomics Consortium...

  6. The SGC Stockholm Collection

    Type
    Collection
    ...Stockholm Collection Structural Genomics Consortium Plasmids Structural Genomics Consortium Plasmids - Stockholm...Structural Genomics Consortium (SGC) Stockholm collection Plasmid... Plasmid Collections Structural Genomics Consortium...

  7. The SGC Toronto Collection

    Type
    Collection
    ...Toronto collection Structural Genomics Consortium Plasmids Structural Genomics Consortium Plasmids - Toronto...Structural Genomics Consortium (SGC) Toronto collection Plasmid... Plasmid Collections Structural Genomics Consortium...

  8. Adenovirus Plasmids

    Type
    Collection
    ...retroviruses , adenoviruses do not integrate into the host genome. Disadvantages of adenoviruses include transient...∆E3-GFP Adenoviral A block for the AdenoBuilder genome assembly system. Block 6 with deletion in E3 and...∆E3-GFP Adenoviral A block for the AdenoBuilder genome assembly system; consolidates the regions present...deltaE1-GFP Adenoviral A block for the AdenoBuilder genome assembly system. Block 1 with deletion in E1 and...plasmid that contains β-gal and GFP; contains ∼10Kb genomic DNA stuffer Vogelstein 18703 pEZYshuttle Shuttle...that span the human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction. ID... insertions into single full-length adenovirus genomes Bunz Return to Top Do you have suggestions for ...

  9. Worm Expression Resources

    Type
    Collection
    ...fluorescence microscopy experiments. The C. elegans genome is well studied and hundreds of reporters have ... be found below: SapTrap-SEC kit for C. elegans genome engineering - Bob Goldstein Lab. Contains a set...from John Calarco's lab as described in Heritable genome editing in C. elegans via a CRISPR-Cas9 system....Co-CRISPR plasmids that facilitate detection of genome-editing events. Developed in Craig Mello's lab ...described in A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis elegans. A modified approach...and distributes stocks of C. elegans. Silencing Genomes - Cold Spring Harbor's guide to RNAi in C. elegans...accessible information concerning the genetics, genomics and biology of C. elegans and related nematodes...

  10. CRISPR Plasmids and Resources

    Type
    Collection
    ...a given genomic locus. Visualize CRISPR fluorescence methods can be used to visualize genomic loci. gRNAs...bacterial immune system to a revolutionary tool for genome engineering. CRISPR Topic Page : Want the latest...experiments. Browse CRISPR Plasmids by Function Genome Engineering Cut Wild-type Cas enzymes generate ...formation. Delete NEW Cascade-Cas3 generates large genomic deletions. Insert NEW CRISPR transposases insert...Screen CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused to an epitope...

  11. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...Cas9 is becoming an ever more ubiquitous tool for genome engineering, and studying its structure can help.... Structural biology of CRISPR–Cas immunity and genome editing enzymes. Nature Reviews Microbiology, 20...

  12. Plasmid Collections

    Type
    Collection
    ...need before starting your experiment. Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs...all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology Pooled...Collection Cell Migration Consortium Impact of Genomic Variation on Function (IGVF) Consortium Krüppel-like... for Solute Carrier Proteins (SLCs) Structural Genomics Consortium Zinc Finger Consortium Pathways & ORFs...

  13. Zebrafish Plasmid Collection

    Type
    Collection
    ...Zebrafish Genome Project - Access the latest reference sequence assembly for the zebrafish genome. Zebrafish... zebrafish research. This includes plasmids for genome editing, empty backbones, fluorescent proteins,... Zon Lab Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system - Shuo Lin ...lineage tracing by combinatorial and cumulative genome editing - Jay Shendure Lab Simultaneous single-...fluorescent proteins for tagging genes at endogenous genomic loci in zebrafish. NTR 2.0 - Jeff Mumm Lab. NTR...Database (ZFIN) - A curated database of genetic, genomic, and developmental data for zebrafish biology. .... Seurat - R package for analyzing single cell genomics in zebrafish. Return to top Do you have suggestions...

  14. Plant Plasmids and Resources

    Type
    Collection
    ...specific modification of monocot and dicot genomes using genome engineering; validated in tomato, tobacco...variety of molecular biology technologies: from genome engineering and gene regulation to fluorescent ...biocontainable RNA virus vectors for non-transgenic genome editing across crop species and genotypes - Zhenghe...system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants - Yao-Guang... virus (TRV) that simplifies gene silencing and genome editing logistics - Jose-Antonio Daros. Return ... agriculture and conservation. Medicinal Plant Genomics Resource (Link opens in a new window) - Transcriptomic...

  15. Brain Initiative Collection

    Type
    Collection
    ...Gordon Fishell 99120-AAV1 pAAV-ihSyn1-tTA An AAV genome that expresses tTA from the hSyn1 promoter that...Gradinaru 99121-AAV1 pAAV-ihSyn1-DIO-tTA An AAV genome with Cre-dependent expression of the tTA from the...Gradinaru 99130-AAV1 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAV2 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAV8 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAV9 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAVrg pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...

  18. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...Protein-DNA Interactions CRISPRainbow and Genome Visualization CRISPR Between the Genes: How to Experiment...methods and tools for studying chromatin and the epigenome. If you have been considering some new techniques...Regulation CRISPR 101: Epigenetics and Editing the Epigenome CUT&RUN: An Improved Method for Studying Protein-DNA...Experiment with Enhancers and Epigenomics I would like to thank the many scientists with me at the Keystone ...
Showing: 361 - 380 of 535 results