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  1. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...Antibody Fragments. Antibodies 8(2):28. https://doi.org/10.3390/antib8020028    Wessel GM, McClay DR (1986...Cytochem : Off J Histochem Soc 34(6):703–706. https://doi.org/10.1177/34.6.3084626  Zhang Q, Miyamoto A, Watanabe...microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016/j.crmeth.2022.100301  Additional resources... the Mouse on Mouse Mess in IHC Resources on Addgene.org  Browse ready-to-use antibodies at Addgene...

  2. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post
    ...integrins (cell-adhesion proteins) targeting different organs. This means that exosomes not only promote microenvironment...microenvironment’s malignancy but also incite different organs to become malignant. Exosomes also contain miRNA..., et al. "Tumour exosome integrins determine organotropic metastasis." Nature 527.7578 (2015): 329. PubMed...about the tumor microenvironment Resources on Addgene.org Visit our cancer research plasmids and resources...

  3. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...can take to verify your own plasmids. Staying organized With hundreds of samples coming through our doors...first step to ensuring plasmid quality is to stay organized. Here at Addgene, we collect data on every plasmid...selection markers. You don’t need a fancy database to organize your own plasmid collection, although you will...found here. Finally, you will want to have a well-organized database of expected sequences, either for the...

  4. The Future of Research Symposium Boston 2015

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    Blog Post
    ...guest blogger and Future of Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar discusses...and for registration please go to http://futureofresearch.org/boston/. The Future of Research Symposium...at the Andrew Young School of Policy Studies at Georgia State University, is an expert on the academic ...experience from the perspective of both grassroots organisations that represent postdocs and institutes that ...

  5. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post
    ... 27649177. PubMed Central PMCID: PMC5038762. 3. George Abraham, Bobin., et al. "Fluorescent protein based...physical stresses or even apply forces (Gayrard and Borghi 2016) to a selected protein. Overcoming current...PubMed PMID: 27475975. 6. Gayrard, Charlène, and Borghi, Nicolas. "FRET-based molecular tension microscopy...FRET Based Biosensors Additional Resources on Addgene.org Find FRET Pairs Check Out Fluorescent Protein...

  6. CRISPR in the Clinic

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    Blog Post
    ...functional correction of the gene in clonally expanded organoids. This work, and many others, pushed the field ... post was originally written in 2014 by Kendall Morgan and updated in 2023 by Susanna Stroik.  References...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...1563. doi: 10.3390/ijms24021563 Resources on Addgene.org CRISPR Plasmids and Resources CRISPR 101 eBook...

  7. Viral Vectors 101: Calcium Sensors

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    Blog Post
    ...concentrations and transients in living cells and organisms. Unlike chemical indicators, these DNA constructs...constructs can be delivered to intact tissues and organs noninvasively and target a defined population of cells...Genetically encoded fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep...protocols. 2006 Aug;1(3):1057-65. Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: ...

  8. High Complexity Golden Gate Assembly with NEB’s DAD

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    Blog Post
    ...lac operon assembly deposited by NEB – and don’t forget to ask DAD for some help! References Potapov,...to DNA Assembly. ACS Synthetic Biology. https://doi.org/10.1021/acssynbio.8b00333 Pryor, J. M., Potapov...ACS Synthetic Biology, 11(6), 2036–2042. https://doi.org/10.1021/acssynbio.1c00525 Pryor, J. M., Potapov...assembly design. PLOS ONE, 15(9), e0238592. https://doi.org/10.1371/journal.pone.0238592    ...

  9. Early Career Researcher Toolbox: Finding Relevant Papers

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    Blog Post
    ... time to read them. Organizations: Accounts for nonprofits, research organizations, and universities often...hashtags to library entries, which can help you organize and search for papers later. Pro tip Learn more...search terms will show up in your feed. Feeds are organized by papers published each day, week, or month. ...

  10. Hot Plasmids: Summer 2025

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    Blog Post
    ...imaging. Nature Communications, 16(1), 3241. https://doi.org/10.1038/s41467-025-58223-5.   TnpB, so hot right...Arabidopsis. Nature Plants, 11(5), 967–976. https://doi.org/10.1038/s41477-025-01989-9.   Make a splash with...mutations. Cell Stem Cell, 32(4), 564–580.e6. https://doi.org/10.1016/j.stem.2025.01.012.  New AAV6 and AAV11...tracer. Nature Methods, 22(5), 1101–1111. https://doi.org/10.1038/s41592-025-02670-x.  ...

  11. Hot Plasmids and Viral Preps - May 2021

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    Blog Post
    ...2020. https://doi.org/10.1016/j.cell.2020.08.012  Starr et al Science. 2021. https://doi.org/10.1126/science.abf9302... Journal of Biological Chemistry 2020. https://doi.org/10.1074/jbc.RA120.01289     Novel protein cage...pathways.   Cheah et al., bioRxiv 2021. https://doi.org/10.1101/2021.01.30.428974   The CRISPR corner...

  12. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post
    ...in 720°, i.e. they are on a membrane or in an organelle, then their effective concentration will be much...the membranes into stacked structures including organized smooth endoplasmic reticulum (OSER) whorls. 2)...distorted into dense stacked membranes termed Organized Smooth Endoplasmic Reticulum or OSER if a dimerizing...in Your Experiments Additional Resources on Addgene.org Browse our Fluorescent Protein Resources Find...

  13. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a “perfect repair” like...researchers can exploit for CRISPR gene editing. In organisms where HDR is downregulated, MMEJ represents another...for Your Experiment Additional Resources on Addgene.org Learn All about CRISPR on Our CRISPR Guide Pages...

  14. Light Sheet Fluorescence Microscopy

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    Blog Post
    ...Resources: http://openspim.org/Welcome_to_the_OpenSPIM_Wiki http://www.janelia.org/lab/keller-lab ...specimens and for in vivo imaging of transparent organisms. Advances in tissue clearing Similar to light ...3DISCO (3-dimensional imaging of solvent cleared organs) method of imaging rodent brains and spinal cords...

  15. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post
    ...a co-founder of the BioBricks Foundation, an organization working to advance biological engineering openly... technical challenge. We have a strategy that organizes how we start the work, and it begins with the ...which we know and love. Boolean is in reference to George Boole who in 1854 was thinking about logic and ... Kendall Morgan...

  16. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post
    ...number of factors, including the age of the model organism, injection site, and titer. Addgene’s Viral Vector...production. Neuromethods, 195, 77–101. https://doi.org/10.1007/978-1-0716-2918-5_5 Haery, L., Deverman.... Frontiers in Neuroanatomy, 13. https://www.frontiersin.org/articles/10.3389/fnana.2019.00093 McDade,...Molecular Biology, 115, 31.5.1-31.5.13. https://doi.org/10.1002/cpmb.8...

  17. Lab to Office Culture Shock

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    Blog Post
    ... number of career paths--industry, nonprofit organization, law office, journal editor, etc.--so everyone...around the world, such as BosLab here in Boston. Organizations like this one are always looking for scientists... own independent research!). There are also organizations like Science from Scientists, which try to bring...blog posts about career readiness Resources at Addgene.org Find careers at Addgene Watch out career videos...

  18. Antibiotic Resistance: An Old Solution but a New Problem

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    Blog Post
    ... that the drug prevents the growth of a living organism. As for multi-drug resistance: definitions vary...vary in the literature, but generally organisms with multi-drug resistance are resistant to three or more...is an intense amount of competition between microorganisms for available resources. This competition drives...Our Microbiology Pages Additional Resouces on Addgene.org Check Out Our Microbiology Pages ...

  19. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Saudi Arabia. Bioinformation, 14, 53–59. https://doi.org/10.6026/97320630014053 Barlow-Anacker, A., Bochkov...cell activation. PLOS ONE, 12, e0180664. https://doi.org/10.1371/journal.pone.0180664 Boix, F., Llorente... Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23 Jhunjhunwala, S., Aresta-Dasilva...Implant Materials. PloS One, 10, e0137550. https://doi.org/10.1371/journal.pone.0137550 More resources on ...

  20. Hot Plasmids - August 2020

    Type
    Blog Post
    ... Sanchez MI et al., Nat methods. 2019. https://doi.org/10.1038/s41592-019-0665-7 Optimizing fluorescent... Chertkova, AO, et al., bioRxiv. 2020. https://doi.org/10.1101/160374 Nanobodies- there's a simple method...Iwaki et al., Protein Expr Purif, 2020. https://doi.org/10.1016/j.pep.2020.105607 A new multiplexed CRISPRi... Hazelbaker et al., Sci Reports. 2020. https://doi.org/10.1038/s41598-020-57500-1 The CRISPR corner By...
Showing: 361 - 380 of 949 results