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We narrowed to 416 results for: plasmids 101

Showing: 361 - 380 of 416 results

  1. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...freezer, you’ll probably find lots of different plasmids containing the same protein of interest tagged...Addgene’s collection, so you may be able to get a plasmid ready-to-use without any cloning at all! As mentioned...Chemical Biology, 26(4), 584-592.e6. https://doi.org/10.1016/j.chembiol.2019.01.003 Gallagher, S. S., Sable,...Chemistry & Biology, 15(2), 128–136. https://doi.org/10.1016/j.chembiol.2008.01.007 Griffin, B. A., Adams, S...

  2. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    .... elegans. These modular toolkits build single plasmid targeting vectors that encode the gRNA transcript... C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives It is interesting to...Precision Genome Editing. BioRxiv. https://doi.org/10.1101/354480 Ghanta KS, Mello CC (2020) Melting dsDNA...elegans. Methods 121–122:86–93 . https://doi.org/10.1016/j.ymeth.2017.03.023 Paix A, Wang Y, Smith HE, Lee...

  3. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...get right. Here at Addgene, we use a linearized plasmid standard. However, even when the standard is made... could end up with a titer of 4 x 1012 genome copies/mL or 8 x 1012 genome copies/mL and both results ... Since AAV titers tend to be in the range of 1012 to 1013 GC/mL, you must serially dilute your virus before...

  4. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ... Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent Protein Guide: Biosensors... Autophagy Research. Cell,140(3), 313-326. doi:10.1016/j.cell.2010.01.028. PubMed PMID: 20144757. PubMed... and Non-Mammalian Systems, Part A, 33-42. doi:10.1016/s0076-6879(08)03203-5. PubMed PMID: 19185711. Additional...

  5. RNA Extraction Without A Kit

    Type
    Blog Post
    ...on the Addgene blog Read blog posts related to plasmid protocols Find protocols for purifying DNA without...BBA) - Enzymology 341:484–488. https://doi.org/10.1016/0005-2744(74)90240-x Chirgwin JM, Przybyla AE, ...Analytical Biochemistry 162:156–159. https://doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi N (2006...

  8. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences from a given...Activation. Cell 159:647–661 . https://doi.org/10.1016/j.cell.2014.09.029 Konermann S, Brigham MD, Trevino...Screens. Cell Systems 6:555-568.e7 . https://doi.org/10.1016/j.cels.2018.04.011 Polstein LR, Gersbach CA (2015...Fluorescence Imaging. Cell 159:635–646 . https://doi.org/10.1016/j.cell.2014.09.039 Wong N, Liu W, Wang X (2015)...

  9. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...in analysis Plasmid-based proteins: plasmid sequence not accurate Check plasmid sequence for frameshifts...Toxicological Methods, 64, 233–237. https://doi.org/10.1016/j.vascn.2011.06.001 More resources on the Addgene...

  10. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ... The most obvious workaround is to use a multi-plasmid system, where you have one AAV vector dedicated...Molecular Therapy, 30(12), 3515–3541. https://doi.org/10.1016/j.ymthe.2022.09.015 Ramani, B., Rose, I. V., Teyssier...Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha, A. J., Klingler, E....

  12. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Cell-Imaging Plasmid Collection New England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental...needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector SNAP-tag...Control Plasmid SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid SNAP-tag...Control Plasmid CLIP-tag Control for mitochondrial localization 101125 pCLIPf-NK1R Control Plasmid CLIP-...Control Plasmid ACP/MCP-tag Control for cell surface localization 101128 pACP-ADRβ2 Control Plasmid ACP/MCP-tag...Biolabs has deposited plasmids at Addgene to generate SNAP, CLIP, ACP or MCP tagged plasmids for visualization...localization 101129 pSNAPf-Cox8A Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B...

  13. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ... RJ, Paterson ES, Smith ML. The copy-number of plasmids and other genetic elements can be determined by...Microbiol Methods. 2006 Jun;65(3):476-87. doi: 10.1016/j.mimet.2005.09.007. Epub 2005 Oct 10. PMID: 16216354...

  14. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...PMC3314244. Resources at Addgene Find optogenetics plasmids! Find AAV resources! Find fluorescent protein ...neuroscience. 2011;34:389-412. doi: 10.1146/annurev-neuro-061010-113817. PMID: 21692661.  3. Bernstein JG, Boyden... cognitive sciences. 2011;15(12):592-600. doi: 10.1016/j.tics.2011.10.003.  PMID: 22055387;  PMCID: PMC3225502...Progress in brain research. 2012;196:193-213. doi: 10.1016/B978-0-444-59426-6.00010-0. PMID: 22341327; PMCID... consumption. Neuron. 2012;73(6):1184-94. doi: 10.1016/j.neuron.2012.02.016. PMID: 22445345; PMCID: PMC3314244...

  15. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Khakh Discontinued Penn Vector Core Viral Vectors (Plasmids still available, other serotypes may be available...available, click on the links for more information. Plasmids are available for all the items listed below. ...pAAV.CMV.Luc.IRES.EGFP.SV40 James M. Wilson AV-9-PV1262 Plasmid not received AV-9-PV1522 100056-AAV9 pAAV.EF1a.....ChR2-YFP.WPRE.hGH Karl Deisseroth AV-9-PV1814 Plasmid not received AV-9-PV2055 105546-AAV9 pENN.AAV.CB7...PV4703 101060-AAV1 pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Biosensor Eric Schreiter AV-1-PV4704 101064-AAV1... Zeng AV-2-PV0101 105530-AAV2 pAAV.CMV.PI.EGFP.WPRE.bGH Control James M. Wilson AV-2-PV0101 105530-AAV2...-WPRE-SV40 Biosensor Eric Schreiter AV-1-PV4705 101061-AAV1 pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40...

  17. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid Collection You May Also...full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science...induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited...distribution to the research community. With these plasmids researchers will be able to generate fluorescently...Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells Find additional cell...

  18. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...in mammals Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral...Trends Mol Med. 2018, S1471-4914(18)30123-0. DOI: 10.1016/j.molmed.2018.06.006. PubMed PMID: 29983335. 9....

  19. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Packaged on Request AAV Guide Plasmids Publications Enhancer AAV plasmids are plasmids which use genomic regulatory...Find AAV enhancer plasmids for fluorescent labeling and recombination across brain regions and populations...) . Allen Institute for Brain Science Enhancer Plasmids The Allen Institute for Brain Science Enhancer...Enhancer Collection contains best-in-class AAV plasmids to target a variety of cell populations in different...tested in mouse brains. This collection contains plasmids for cell populations in the following regions:...Hypothalamus Cerebellum Whole brain Addgene ID Plasmid Allen Institute ID Enhancer ID FP/Recombinase Labeled...AiP12040 AiE0354m FlpO Vip interneurons Isocortex 230101 pAAV-hsA2-AiE0219h-minRho-SYFP2-WPRE3-BGHpA AiP11712...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...after transfecting with the transfer and packaging plasmids. A benefit of the multiple harvest strategy is... Gene Therapy 5.4 (2005). 387-98. Pubmed PMID: 16101513. PubMed Central PMCID: PMC1368960. 10. Denning...
Showing: 361 - 380 of 416 results