We narrowed to 883 results for: prot

...detect, purify, quantify, deplete, and visualize proteins of interest (Greenfield, 2014). Traditionally,...chain for the antibody and when translated into protein will assemble into a fully functional antibody....encoded in a plasmid. (Right) An assembled antibody protein binds an antigen. Image from Fvasconcellos. ...genes for antibody expression. If starting from a protein sample (for instance, affinity purified antibodies...well in animals because of similarities to host proteins. After the antibody of interest has been cloned...

...Confirming tags and fusion proteins Finally, we confirm promoters, tags, fusion proteins, and selectable markers...activation screening. Nat Protoc 12:828–863 . https://doi.org/10.1038/nprot.2017.016...entry or using BLAST.   Confirming tags and fusion proteins. We’ll break down each of these steps below. ...

...bacteria CRISPR-Casɸ mGold, a new yellow fluorescent protein New CRISPR plasmids New items from the viral service...use light to induce conformational changes in a protein.    Cells expressing an RFP reporter plasmid...Cre, Opto-Cre-Vvd, by splitting and joining the protein regions with Vivid (Vvd) photodimers. Upon the ...mGold, the most photostable yellow fluorescent protein to date By Justin Ng High-throughput screening ...types expressing variants of Yellow Fluorescent Protein (YFP). The team identified a new YFP variant, mGold...

...incomplete. Protein expression strains are not good for cloning Bacterial strains adapted for protein purification...levels of protein, not plasmid DNA. If you need large amounts of DNA from a plasmid in a protein expression...a DNA prep kit or your lab's favorite in-house protocol. DNA yields for this procedure are typically in...

...DNA N-glycosylase (UNG) enzymes. Various other proteins included in different CGBE tools allow the researcher... human AAG (remember, MPG and AAG are the same protein!) (Chen et al., 2024). They called their initial...considerably. And there’s so much more exciting protein engineering going on in the base editing field!...hypoxanthine from DNA, creating an abasic site. The same protein (or its homologs) has more than one name. Indel...Huang, Q., Ma, Y., Yuan, F., & Chang, X. (2024). Protein language models-assisted optimization of a uracil-N-glycosylase...

...generally validate our viral vector preparation protocol, and we are pleased to report that over 95% of...Primer annealing can be affected by the presence of protein contaminants, which is a factor in AAV titration...viral vector solution (which contains viral capsid proteins) as opposed to purified DNA. This may be mitigated...preps contain similar and low levels of background protein and chemical components, ddPCR may not be an improvement...More about Virus Production Find Viral Vector Protocols ...

...based on what happens to an organism when a gene/protein is depleted in a knockout experiment. Certain scientific... break room for Addgenies, was named after the protein Groucho, a transcriptional co-repressor important...important in development. Groucho family proteins are found in flies, worms, frogs, mice, and even humans. ...Nusslein-Volhard and Wiechaus, 1980). The mammalian hedgehog protein, sonic hedgehog (SHH) was named by a postdoc in...including oogenesis and cytokinesis. Why name this protein after a vegetable substitute for spaghetti? I actually...

... vague and "what proteins regulate the Z pathway?" is an open-ended list of proteins. Instead, consider...of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent proteins,  and live cell...consider "how do proteins X and Y regulate the Z pathway?" A well-defined outcome makes it easier for the search...

...that affected gene expression, mRNA splicing, or protein coding regions. In total, they tested 13 strains...phenotype, but only one, Ly6a, showed differential protein and RNA expression between the brains of C57BL/...similar to the Deverman Lab when they looked at LY6A protein and RNA expression patterns, the PHP.B permissive...17379763. Hordeaux, Juliette, et al. "The GPI-Linked Protein LY6A Drives AAV-PHP. B Transport across the Blood-Brain...Find ready-to-use AAV from Addgene or check out protocols for packaging your own virus ...

...label neuronal morphology (eYFP) and the synaptic protein PSD95 (PSD95-mcherry) under the control of Cre ...fluorescent cytoplasmic markers (e.g. eYFP) and synaptic proteins (e.g. postsynaptic PSD95-mcherry) are used in ...In order to analyze the function of individual proteins in neuronal circuits or synaptic remodeling, researchers...be induced by Cre recombinase expression while proteins needed for later experiments (cellular tracking...is fascinated by the question of how genes and proteins coordinate billions of neurons to form the functional...

...elegans. Other groups have used Mango to study RNA-protein complexes (Panchapakesan et al., 2017) and short...selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution... Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification and characterization using RNA...highly efficient RNA mimics of green fluorescent protein." Nature structural & molecular biology 21.8 (2014... Resources at Addgene.org Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids...

...reporter constructs), in functional analysis of proteins (to perform alanine scanning mutagenesis or targeted...using primers (with the desired mutation) in a PCR protocol that amplifies the entire plasmid template. The...simply following the polymerase manufacturers’ PCR protocol. Be sure to adjust the extension time to match...sites. Alternatively, if you want to make your protein non-functional, you may simply introduce a frame-shift... on Addgene.org Browse Our Molecular Biology Protocols Find Empty Vectors for Your Next Cloning Experiment...

...detection because they produce very little or no viral protein. To solve this problem, two strategies have been... HIV so that infected cells will produce viral proteins and die, either through cytotoxicity or the immune...expression. In cell lines that can produce HIV-1 toxic proteins, CRISPR/Cas9 SAM caused apoptosis, indicating ...However, this approach could permit some viral protein production, so researchers are also interested ...antiviral therapies target HIV-1 at the level of protein structure, CRISPR gRNAs require DNA sequence-specific...

...Plasmid Cloning Protocols Find Virus Protocols Find Basic Molecular Biology Protocols Gibson Assembly...can be mixed yourself (e.g. see the Miller Lab Protocol). The Gibson assembly process can be used to assemble...commitments. These kits also come with specific protocols, suggestions for ratios of product to insert, ...

...uses the direct injection of a CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair templates...development of CRISPR/Cas9 editing methods, other Cas proteins have been adapted for use in C. elegans. In 2017... mammalian and yeast cells using modified Cas9 proteins to regulate gene expression (CRISPRi/CRISPRa),... genomic loci (CRISPR-imaging), or to find the proteins associated with a given genomic locus (CRISPR-...improve genome editing and conditionally manipulate proteins. Learn more at his webpage or follow him on Twitter...

...in a direct or unbiased way at the DNA, RNA, or protein level. The “correct” choice of tool requires careful...unmatched—unlike CRISPR experiments that require exogenous protein, successful induction of RNAi only requires the...scored. However, determining the transcript or protein abundance of your specific target required physical...possible in plants outside of limited, single cell protoplast analysis. Instead, microscopic plants must be...Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based Artificial MicroRNA Screens for Effective...

... in skeletal muscle by exercise and endurance, protects the brain from depression. Mice that are genetically...stressors as usual, but somehow their brains were protected. Effects of Activation of the PGC-1a1 Pathway ...levels of kynurenine in the body, the brain is protected from stress-induced biochemical changes that could...explore. “It will be important to identify the key proteins and metabolites that are dynamically secreted ...

...encodes for a small Cas protein (40-70 kDa), which is half the size of other Cas proteins found in the so-called...adaptive immune systems that provide bacteria protection against infectious agents. Several enzymes behind... of the larger and more complex Cas9 and Cas12 proteins. Cas14 cleaves ssDNA But what is the function ...

...in government, evidence to inform policies, a protected place to work, funding for science, and numerous...ultimately galvanized scientists to rally in Ottawa to protest the “death of evidence,” and to speak out in unprecedented...water. Many marchers held signs demanding the protection of our Great Lakes and our planet. And as we ...Nashville, TN, USA). I had never participated in a protest before, so I had no idea what to expect. Admittedly...although there was at least one person vocally protesting us, he was not threatening in any way. Participating...

...Your Fluorescent Proteins for Multi-Color Imaging Choose the Brightest Fluorescent Protein for Your Experiments...Addgene.org Browse Our Fluorescent Protein Resources Find Empty Fluorescent Protein Backbones Find Plasmids for... fluorescent proteins...