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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 160 results for: ALC

Showing: 21 - 40 of 160 results

  1. Brain Initiative Collection

    Type
    Collection
    ...-mCherry-hPMCA2w/b Expresses a plasma membrane calcium pump hPMCA2w/b Baljit Khakh 112000-AAV1 pAAV-hSynapsin1...135420-AAV1 pAAV-syn-FLEX-jYCaMP1s Yellow protein calcium sensor expressed under human Synapsin1 promoter...pAAV-syn-FLEX-axon-jYCaMP1s Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter...Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s Yellow protein calcium sensor expressed under human Synapsin1 promoter...

  2. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...doi: 10.1101/2022.08.13.503850.  iGECInano NIR calcium reporter Near-infrared (NIR) fluorescent proteins...’s lab has generated a new genetically encoded calcium indicator using small monomeric NIR fluorescent...photochemical characteristics than previous NIR calcium reporters iGECI and NIR-GECO2G, and its small size...Characterization of a Small Near-Infrared Fluorescent Calcium Indicator. Front Cell Dev Biol. 2022 Jun 29;10:...response size and reduced sensitivity to pH and calcium compared to previous lactate biosensors, and its...additional AAV serotypes of the most recent jGCaMP8 calcium sensors: pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3...

  3. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...luciferase Many intracellular sensors of events like calcium release have been made using fluorescent resonance...generally consist of a responsive protein (e.g. calcium binding protein) fused to two fluorescent proteins...proteins. When the responsive protein binds to calcium it changes conformation and brings the two fluorescent... due to its bright luminescence. Intracellular Calcium Sensor Yang, J. et al. (7) wished to trigger intracellular...this optogenetic tool, they needed to create a calcium sensor with the following characteristics: No ...CalFlux VNT responds to changes in intracellular calcium and is useful for direct imaging of events in cells...

  4. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...pAAV-hSyn-DIO-EGFP   Biosensor AAV Calcium sensors Tracking calcium dynamics that govern such activities...studying neural networks. We have expanded our calcium sensor collection to include our first Flp-dependent...Several new options are also available for the calcium sensor jGCaMP7 from Janelia, including three jGCaMP7...technology, see our blog post Overview of AAV Encoded Calcium Sensors). Plasmid Serotype Name 104489 AAVrg...

  5. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...tap water. Add ethyl alcohol or acetone drop by drop onto the smear until the alcohol runs clear and immediately... one type, Streptococcus pneumoniae, while the alcohol used in the staining process bleached another, ...peptidoglycan layer, staining the cell purple. When the alcohol is added, it dehydrates the peptidoglycan layer...positive bacteria. In a gram negative bacteria, the alcohol destroys the outer lipid membrane, and the thin...

  6. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...and iGECI calcium indicator (Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators...performance, and applications of near-infrared calcium indicators in this recent review (Shcherbakova ... genetically encoded near-infrared fluorescent calcium ion indicator. Nat Methods 16, 171-174 (2019). ....A. et al. A near-infrared genetically encoded calcium indicator for in vivo imaging. Nat Biotechnol 39...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...Transfer (BRET) CalFlux VNT : An intracellular calcium sensor created by fusing NanoLuc® and Venus fluorophore...interest can also be inserted into the plasmid. Malcolm Moore 51819 Syn-ATP Firefly (modified) CMV Optical...Lentiviral expression of firefly luciferase Javier Alcudia 113442 pcDNA3.1 NL NanoLuc® CMV Mammalian expression...

  8. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...Plasmodium falciparum genome. To put this last point into perspective, the Plasmodium falciparum genome contains...in their work editing the genome of Plasmodium falciparum (the parasite that causes malaria). I attribute...sites, scores and the statistics that go into calculating them. For this, some online tools such as Cas-OFFinder...editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system." Nature biotechnology...

  9. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...tried to find an app that could help me with calculations. This is how I came across the Gibco Cell Culture...culture and include a counter, lab timer, dilution calculator, and a couple of other nice features. This app...sequence for your enzyme of interest. Lastly, the Tm calculator helps you set up an optimal PCR depending on ...apps: Findings Lab Notebook Cell Culture Split Calculator by DRDP Restriction Enzyme Tool by Promega Material...

  10. Hot Plasmids - August 2020

    Type
    Blog Post
    ...fluorescence protein-based calcium sensors (jYCaMP1), variants of the calcium indicator jGCaMP7, that are...with red fluorescence protein-based calcium sensors. These new calcium sensors include syn-jYCaMP1s (slow...

  11. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...15R] QKI-7 Human Mouse IgG2a 177508 Anti-NALCN [N185/7R] NALCN Rat Mouse IgG2a 177509 Anti-Dopamine D2 ...Mouse IgG2b 225412 NALCN (Rattus norvegicus) recombinant monoclonal antibody. NALCN Rat Mouse IgG2b 225413...Alpha1 Human Mouse 206721 NALCN ion channel scFv [N185/7] N185/7 scFv NALCN ion channel Rat Mouse 206722...

  13. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    .... Factor Xa loves calcium, but metal-chelate matrices, not so much. Maybe a calcium-independent proteinase...This allows washing away of unbound material to calculate the amount of bound ligand (after subtracting ...

  14. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...Control AAVs for serotype testing jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic inhibition...high contrast between its resting state and its calcium-bound state, which was achieved by lowering the...  AAV1  pAAV.hSynapsin.SF-iGluSnFR.S72A New calcium sensors Plasmid Serotype Name 104496  AAV1...

  16. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ... followed by a talk from Professor Karin about alcohol and obesity as driving factors for chronic inflammation...chronic hepatitis caused by hypernutrition and alcohol consumption. Chronic inflammation is a dangerous...cells that are not excitable but responsive to calcium. We’d love to expand our cancer and immunology ...

  17. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...robust, with an eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki et al... to previous publications. They also developed calcium, cAMP, and ATP sensors based on Nano-lantern, showing...

  18. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...not use CsCl are commercially available. Titer calculation (1 or 10 days) Physical titer (particle count...260nm corresponds to 1.1 x 1012 particles/mL. To calculate the particle number: OD260 reading x dilution ... days later. Plaques are counted and titer is calculated in terms of plaque forming units (pfu), taking...

  19. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...used to calculate the concentration of the sample. This concentration can then be used to calculate the viral...Maintaining a high droplet count is important for calculating the concentration of your sample. Even if you...

  20. Five Popular Model Organisms

    Type
    Blog Post
    ...Andrew Leifer’s lab utilized the worm to study calcium signaling in the entire adult brain, using a plasmid...plasmid that expresses a genetically encoded calcium sensor, GCaMP6 (Nguyen et al., 2015). If you are ...PMC5349099. Nguyen, Jeffrey P., et al. "Whole-brain calcium imaging with cellular resolution in freely behaving...
Showing: 21 - 40 of 160 results