We narrowed to 114 results for: CHL

1. Biosensor AAV Preps

   Type

   Collection

   ...none Cre dependent 9 Tian 163909 pAAV_hSynapsin_psychLight2 Syn psychLight2 none Constitutive 9 Tian GABA...RdLight GRAB_DA GRAB_rDA GRAB_gDA Serotonin Sensors psychLight iSeroSnFR GABA Sensors iGABASnFR iGABASnFR2 Glutamate...

2. AAVED

   Type

   Collection

   ...Harvard Medical School Dr. Melina Fan, Dr. Susanna Bachle, Dr. Karen Guerin, Dr. Leila Haery, Dr. Meghan ...Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J, Fan M. Front Neuroanat. 2019 Nov ...

3. Plasmid Preps: Different Purity, Different Quantities, Different Uses

   Type

   Blog Post

   ...agents as guanidine thiocyanate and guanidine hydrochloride used during plasmid extraction. Figure...

4. University of Florida Serotype Testing Panel for the Eye and Brain

   Type

   Collection

   ...application TMN200 (200 mM sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable for...

5. Microbes: Look, Listen, and Tell

   Type

   Blog Post

   ... Susanna Bachle...

6. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

   Type

   Blog Post

   ...By Susanna Bachle and Matt Takvorian When COVID-19 researchers started working with us to help them share...

7. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

   Type

   Blog Post

   ... Susanna Bachle...

8. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

   Type

   Blog Post

   ...but when I met the astronaut Jim Lovell I was speechless (the club of people who have been in space is...

9. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

   Type

   Blog Post

   ... Susanna Bachle...

10. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type

    Blog Post

    ...lab and Takafumi Minamimoto’s lab known as deschloroclozapine (DCZ) has been developed to meet these requirements...

11. Plasmids 101: Antibiotic Resistance Genes

    Type

    Blog Post

    ...elongation step) Bacteriostatic 10 ug/mL Chloramphenicol   Binds 50S ribosomal subunit; inhibits peptidyl...

12. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type

    Blog Post

    ...genomic locus: Caspex  Article contributed by Susanna Bachle  Listen to the Caspex podcast segment Steven Carr...

13. Plasmids 101: Origin of Replication

    Type

    Blog Post

    ...amplifying more copies with the addition of chloramphenicol – make sure your growth conditions aren't working...

14. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type

    Blog Post

    ...Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J, Fan M (2019) Adeno-Associated Virus...

15. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type

    Blog Post

    ...sgRNA iBAR library Article contributed by Susanna Bachle Figure 1: Initial steps in the CRISPR-pooled...

16. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type

    Blog Post

    ...References and Resources References Romano E, Baumschlager A, Akmeriç EB, Palanisamy N, Houmani M, Schmidt...

17. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type

    Blog Post

    ...optogenetic toolbox Article contributed by Susanna Bachle Anion-conducting channelrhodopsins (ChR) are ion...

18. Summer SciComm Series: A PhD in Science Communication

    Type

    Blog Post

    ...poring over my grandparents’ medical textbooks by torchlight under the covers. In high school, I went to all...

19. Plasmids 101: Gram Negative and Positive Bacteria

    Type

    Blog Post

    ...types. In water, the dye dissociates into CV+ and Chloride (Cl-) ions. When the negatively charged iodine...

20. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type

    Blog Post

    ...By Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources...

21. R Bodies: Membrane-Rupturing Microscopic Tools

    Type

    Blog Post

    ...Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive extrusive...

22. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type

    Blog Post

    ... Susanna Bachle...

23. Multiplex Genome Editing with CRISPR-Cpf1

    Type

    Blog Post

    ...Abudayyeh, O., Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015). Cpf1 Is a Single RNA-Guided...

24. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type

    Blog Post

    ...in the lab. This is commonly done using calcium chloride which permeabilizes the cell membrane so the bacteria...

25. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type

    Blog Post

    ... Susanna Bachle...

26. Antibodies 101: ChIP

    Type

    Blog Post

    ... with a PCR purification kit or with a phenol-chloroform extraction.    Fig. 1: Schematic overview...

27. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type

    Blog Post

    ...is in citizen science and behavioral ecology of cichlid fishes. Find David online at https://orcid.org/...

28. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type

    Blog Post

    ...active LacZ tetramer.  This tetramer cleaves chlorophenol red-b-D-galactopyranoside on the assay paper...

29. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    ...expected full plasmid sequences (examples include Benchling, Serial Cloner, and Snapgene), and save these ...

30. Working with Nuclear Receptors

    Type

    Blog Post

    ...reporter gene has over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic ...