We narrowed to 45 results for: RNAi
-
TypeBlog Post...bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression, worm expression...
-
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog Post...translation either by blocking initiation or splicing. (RNAi, in contrast, engages existing gene regulatory mechanisms... -
Plasmids 101: Control Plasmids
TypeBlog Post...targeting or genome editing technologies, such as RNAi or CRISPR, non-targeting controls may be appropriate... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...It’s possible to actually silence this gene with RNAi to make a polygamous species monogamous. Anolis ... -
Antibodies 101: Validation
TypeBlog Post...expected protein. Common approaches include using RNAi to knock-down expression of your target or CRISPR... -
Important Considerations When Using AAVs
TypeBlog Post... Kay. "Future of rAAV gene therapy: Platform for RNAi, Gene Editing and Beyond." Human Gene Therapy ja... -
Tetracycline Inducible Expression
TypeCollection...Elledge 111177 LT3GEPIR Lentiviral Tet-On inducible RNAi with miR-E (miR-30 variant)-based backbone rtTA-... -
CRISPR History and Development for Genome Engineering
TypeCollection...skyrocketing popularity of CRISPR echoing that of RNAi and iPS cells. Genome engineers continue to work... -
Lentiviral Vector Guide
TypeGuide...). Lentivirus-delivered stable gene silencing by RNAi in primary cells. RNA , 9 (4), 493–501. https://... -
Delivery Methods for Generating iPSCs
TypeBlog Post... gene-specific plasmids (including reporters and RNAi) for: NANOG OCT4 SOX2 MYC KLF4 LIN28 Check ... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...Initial steps in the CRISPR-pooled screening using sgRNAiBAR. Image from Zhu et al., 2019. Wensheng Wei's...their loop region. After infecting cells with sgRNAiBAR library containing lentiviral vectors the iBARs...the gRNAs and their iBAR sequences. Since the sgRNAiBAR library covers every annotated human gene, it ...number of cells and in vivo settings. Find the sgRNAiBAR library! Zhu et al., Genome Biology, 2019. PubMed... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...can be used to detect specific phosphoinositides (Varnai and Balla, 2008) (Figure 2). RNA production can...28533371. PubMed Central PMCID: PMC5468623. 11. Várnai, Péter, and Tamas Balla. "Live cell imaging of ... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog Post...Find the BioID Plasmids Here! Macneill, S., & Varnaitė, R. (2016). Meet the neighbors: Mapping local ... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post...https://doi.org/10.1089/hum.2019.277 Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine...