We narrowed to 109 results for: Atr

1. Tag Your Favorite Yeast Genes with Ease

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   ...JR. Yeast. 1998 Jul;14(10):953-61. Note: Marcy Patrick contributed to the writing of this article....

2. pSiM24: Simplifying Plant Genetic Engineering

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   ...Transgenesis with GreenGate Plasmids Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More Plasmids...

3. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

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   ...replications as in this ReFigure about whether Natronobacterium gregoryi Argonaute has any DNA editing functionality...

4. FastCloning: A Newer, Simpler Cloning Technique

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   ... in protein science.     References: 1. Celie, Patrick HN, Annabel HA Parret, and Anastassis Perrakis....

5. Plasmids 101: What is a plasmid?

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   ...please let us know in the comments. Note:Marcy Patrick contributed to the writing of this article. ...

6. Plasmids 101: Antibiotic Resistance Genes

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   ... Marcy Patrick...

7. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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   ... the cell surface Article contributed by Marcy Patrick  Listen to the nanobody toolkit podcast segment...

8. Plasmids 101: Origin of Replication

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   ... do you choose? Addgene Senior Scientist Marcy Patrick says researchers can ask themselves a few questions...

9. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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   ... Marcy Patrick...

10. Donations from Addgene to Yield Answers for Rare Disease Researchers

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    ...with one form of Waardenburg syndrome (WS4) by Pediatrician Duangrurdee Wattanasirichaigoon carried different...

11. Celebrate National Book Month with Addgene

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    ..., there is always another secret."     - Marcy Patrick, Associate Director of Quality Control Jonathan...

12. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    ...one of the more common open-access tools, while EpiMatrix is a commercially available algorithm to evaluate...

13. R Bodies: Membrane-Rupturing Microscopic Tools

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    ...other barriers (like vasculature or extracellular matrices) in response to pH changes. Beyond these applications...

14. Plasmids 101: Mammalian Vectors

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    ... Marcy Patrick...

15. Multiplex Genome Editing with CRISPR-Cpf1

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    ...CRISPR Nucleases   Cas9 Cpf1 Components crRNAtracrRNACas9 crRNACpf1 Endonuclease size spCas9:...

16. Plasmids 101: Methylation and Restriction Enzymes

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    ... Marcy Patrick...

17. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    ...structural protein of the retroviral particle containing Matrix, Capsid, and Nucleocapsid components. Pol ...

18. Viral Vectors 101: Viral Vector Elements

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    ...This is the role of Gag proteins: they contain the matrix, capsid, and nucleocapsid viral components needed...

19. Viral Vectors 101: Viruses as Biological Tools

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    ...diseases, including hemophilia B and spinal muscular atrophy.   Type of Virus Pros Cons Retrovirus ...

20. Plasmids 101: Yeast Vectors

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    ...  Marcy Patrick...

21. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    ... scientists began focusing on the extracellular matrix and fibroblasts. With each cell mechanism discovered...

22. Plasmids 101: Modular Cloning

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    ...Gate Assembly with NEB’s DAD Interview with Nicola Patron on MoClo Plasmids 101: Golden Gate Cloning ...

23. 10 Basic tips for mammalian cell culture

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    ...PubMed Central PMCID: PMC4047077. 2. Pagliara, Patrizia, et al. "Ostreopsis cf. ovata induces cytoskeletal...

24. AAVs in Retinal Gene Therapy

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    ...hemophilia, muscular dystrophy, spinal muscular atrophy, neurodegenerative diseases and metabolic diseases...

25. SciComm: Taking Science to Elementary and Middle Schools

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    ...Family  Biology and Chemistry activities from Quatr.us Foldscopes Steve Spangler Science The Fab Lab ...

26. An Introduction to Adenovirus

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    ...noticing unusual cases of acute hepatitis in the pediatric population. Once ~400 cases had amassed in places...

27. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    ...to thank fellow Addgenies Joanne Kamens, Marcy Patrick, Aliyah Weinstein, Fabienne Furt, and Quintin Marcelino...

28. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ... Marcy Patrick...

29. Your Lentiviral Plasmid FAQs Answered

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    ... to answer them! Note: A. Max Juchheim, Marcy Patrick, and Meghan Rego contributed to the writing of ...

30. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    ...Laboratory Golden Gate Cloning videos made by the Nicola Patron group, one of the developers of the MoClo system...

31. Hot Plasmids: Fall 2024

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    ... & Randall, R. E. (1992). Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency...

32. Twenty Years of Sharing: Addgene's Viral Vector Service

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    ...DeMaio, K. H., Tillgren, M., Fava, A., Clouse, G., Patrick, M., Guerin, K., & Fan, M. (2023). Quality control...

33. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    ...Katherine W. Rogers is a postdoctoral researcher in Patrick Müller’s group at the FriedrichMiescher Laboratory...

34. Save Time with Transient Plant Leaf Transformations

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    ...plants, check out this interview with the Nicola Patron, who shared the MoClo plant kit via Addgene. For...

35. Plasmids 101: Biotinylation

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    ...technology to improve cell adhesion on nanofibrous matrices.” Journal of Nanobiotechnology 13.1 (2015): 1–...

36. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    ...Yiping Qi lab and the MoClo Plant Parts Kit from the Patron Lab.  As with all plasmids in the repository, we...

37. An Integrin Antibody Toolkit from IPI

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    ...development, and the shaping of the extracellular matrix. During immune responses, this same conformation...

38. CRISPR 101: Homology Directed Repair

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    ...Originally written by Marcy Patrick and Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul ...

39. Plasmids 101: Restriction Cloning

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    ...purification, you use a voltage difference across a gel matrix (usually agarose) to pull your negatively charged...

40. Finding Your Perfect Job After University

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    ... genetics of Wilms Tumour, which is a type of pediatric kidney cancer. This was particularly interesting...

41. Allen Institute for Cell Science Plasmid Collection

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    ...Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin Matrix Adhesions 87421 TUBA1B-mEGFP AICSDP-4 mEGFP Alpha-tubulin...

42. Sequencing Options for CRISPR Genotyping

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    ...PMID: 27087594. 16. Tycko, Josh, Vic E. Myer, and Patrick D. Hsu. "Methods for Optimizing CRISPR-Cas9 Genome...

43. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    ... and approaches for optogenetics." Biological psychiatry 71.12 (2012): 1033-1038. PubMed PMID: 22480664...

44. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    ...References Bezanilla M, Perroud P ‐F., Pan A, Klueh P, Quatrano RS (2005) An RNAi System in Physcomitrella patens...

45. Control AAV Preps

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    ...pAAV-CBh-mKate2-IRES-MCS (WT-WT) CBh mKate2 Constitutive 2 Patrick 114469 pAAV-CaMKIIa-mCherry CaMKIIa mCherry Constitutive...

46. Typing CRISPR Systems

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    ... Tolstoy, I., Johnson, D. B., Koonin, E. V., & Quatrini, R. (2021). Evolution of Type IV CRISPR-Cas Systems...

47. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    ...by engineering PA-GFP tagged to a mitochondrial matrix protein (mito-PAGFP) the Youle Lab was able to ...

48. Plasmids 101: Modular Cloning Applications and Kits

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    ...express recombinant proteins within the mitochondrial matrix.     Figure 1: A chart with MoClo kit recommendations...

49. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

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    ...biotechnology(2014). PubMed PMID: 24880488. 2. Hsu, Patrick D., et al. "DNA targeting specificity of RNA-guided...

50. Bacterial Expression Systems

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    ...pCyPet-His pYPet-His CyPet (donor) YPet (acceptor) FRET Patrick Daugherty 18084 54856 pBad-mAmetrine1.1 tdTomato-pBAD...