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We narrowed to 73 results for: HDR

Showing: 51 - 73 of 73 results

  2. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...FRT sites, among others. Homology-directed repair (HDR) allows us to create a variety of modifications in...specificity. Figure 2: An overview of CRISPR and NHEJ/HDR. The Cas9/gRNA complex produces a DSB at the target...genomic locus. Repair will proceed through NHEJ or HDR depending on the experimental conditions. Genome ...editing technologies that do not rely on NHEJ or HDR: Base editors : Base editors directly convert bases...break. This method displays higher efficiency than HDR-mediated editing. The first base editors converted...

  4. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...addition to a researcher’s locus of interest. NHEJ- or HDR-induced mutations in ATP1A1 can render cells resistant...editing rates as high as 83% with NHEJ and 40-50% with HDR. Coselection efficiency is so high that some applications...

  6. Hot Plasmids - August 2020

    Type
    Blog Post
    ... (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 kb. Find the plasmids at...

  12. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...designed to introduce a DSB. To learn more about NHEJ, HDR, and other CRISPR topics, read our CRISPR Guide ....

  14. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...than methods relying on homology-directed repair (HDR), and insertion is scarless, unlike previous integrase-based...

  16. Antibody Plasmid Collection

    Type
    Collection
    ...diversification of hybridoma-produced antibodies by CRISPR/HDR genomic engineering. Sci Adv. 2019 Aug 28;5(8):eaaw1822...

  19. Zhang Lab CRISPR Page

    Type
    Collection
    ...plus luciferase-2A-Cre recombinase, and Kras G12D HDR template 60225 : control for AAV-KPL; sgRNA targeted...)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL) This plasmid contains two expression...

  20. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...Elizabeth Hubbard: Neurons involved in opioid withdrawal Elizabeth Hubbard is a fourth year Ph.D. candidate...the development of behavioral signs of opioid withdrawal before they occur.  First, she used a genetic...genetic method to tag neurons activated by opioid withdrawal with tdTomato and assessed cFos expression. She...fluorescence during morphine administration and withdrawal to explore how these cells may encode the negative...negative affect associated with drug withdrawal.  “Together, this project will allow us to better understand... how neuron activity changes following opioid withdrawal, providing insight into new methods for treating...

  21. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...Office, between 1997 and 2000, ten drugs were withdrawn from the US market because of life-threatening...therapies. Let’s go back to why ten drugs were withdrawn from the market between 1997 and 2000. There are...

  22. Hot Plasmids - October 2020

    Type
    Blog Post
    ...indicating a quick cell cycle. Cells that had IL-3 withdrawn became entirely red as they could not proliferate...

  23. Genetic Code Expansion

    Type
    Collection
    ...acyl-lysine derivatives Bacterial Wenshe Liu 127445 pEDF-PhdRS PylRS N346A-C348A M. mazei Bacterial Wenshe Liu ...
Showing: 51 - 73 of 73 results