...FRT sites, among others. Homology-directed repair (HDR) allows us to create a variety of modifications in...specificity. Figure 2: An overview of CRISPR and NHEJ/HDR. The Cas9/gRNA complex produces a DSB at the target...genomic locus. Repair will proceed through NHEJ or HDR depending on the experimental conditions. Genome ...editing technologies that do not rely on NHEJ or HDR: Base editors : Base editors directly convert bases...break. This method displays higher efficiency than HDR-mediated editing. The first base editors converted...

...sequence integrated through homology-directed repair (HDR) (1), or indel mutations resulting from non-homologous...

...enhance DNA repair via homology directed repair (HDR). This characteristic makes AAV vectors especially...

...addition to a researcher’s locus of interest. NHEJ- or HDR-induced mutations in ATP1A1 can render cells resistant...editing rates as high as 83% with NHEJ and 40-50% with HDR. Coselection efficiency is so high that some applications...

...introduce specific edits via homology-directed repair (HDR). Want more information on the wide variety of Cas...

...more than 10 bp away from the insertion site, so HDR will be difficult if there’s no PAM near your insertion...

... (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 kb. Find the plasmids at...

...editing doesn't require homology-directed repair (HDR) machinery, and could thus be used in non-dividing...

...edit a gene using homology directed repair, since HDR-mediated gene editing is most efficient when target...

...protein) upon CRISPR/Cas9 homology directed repair (HDR) (Glasser et al., 2016). For TREE they engineered...

...endogeneous DNA through homology-directed repair (HDR) of a CRISPR/Cas9 induced double-stranded break. ...

...editing methods (most notably CRISPR/Cas mediated HDR) enables the tagging of endogenous genes close to...

...diversification of hybridoma-produced antibodies by CRISPR/HDR genomic engineering. Sci Adv. 2019 Aug 28;5(8):eaaw1822...

...These precise edits tend to be more efficient than HDR (reviewed in Zhu et al., 2019). Both cytosine base...

.... These tools assess repair events by type (NHEJ/HDR) and track indels at a single locus, usually the ...

...eukaryotes in that Cas9-mediated cleavage induces HDR to directly modify a given gene. CRISPR-tranposons...

...plus luciferase-2A-Cre recombinase, and Kras G12D HDR template 60225 : control for AAV-KPL; sgRNA targeted...)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL) This plasmid contains two expression...

...Elizabeth Hubbard: Neurons involved in opioid withdrawal Elizabeth Hubbard is a fourth year Ph.D. candidate...the development of behavioral signs of opioid withdrawal before they occur.  First, she used a genetic...genetic method to tag neurons activated by opioid withdrawal with tdTomato and assessed cFos expression. She...fluorescence during morphine administration and withdrawal to explore how these cells may encode the negative...negative affect associated with drug withdrawal.  “Together, this project will allow us to better understand... how neuron activity changes following opioid withdrawal, providing insight into new methods for treating...

...Office, between 1997 and 2000, ten drugs were withdrawn from the US market because of life-threatening...therapies. Let’s go back to why ten drugs were withdrawn from the market between 1997 and 2000. There are...

...indicating a quick cell cycle. Cells that had IL-3 withdrawn became entirely red as they could not proliferate...

...acyl-lysine derivatives Bacterial Wenshe Liu 127445 pEDF-PhdRS PylRS N346A-C348A M. mazei Bacterial Wenshe Liu ...