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We narrowed to 76 results for: 4-Oct

Showing: 21 - 40 of 76 results

  2. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...FEBS Lett 2012 Oct 19;586(20):3716-22. Park et al., Science 2011 Aug 26;333(6046):1151-4. pOSIP and...transgenesis constructs. Kirchmaier et al., PLOS One 2013 Oct 7;8(10):e76117. Irreversible peptide-peptide ligation... Wang et al., Science. 2014. Jan 3;343(6166):80-4. Shalem et al., Science 2014. Jan 3;343(6166):84...assay. This new Platinum Gate TALEN Kit utilizes a 4-module assembly system, which reduces the number of...Stigleoclonium helveticum (ShChR), Chlamydomonas noctigama (CnChR1), Chloromonas subdivisa (CsChR), Chloromonas...

  4. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... Technologies. Cold Spring Harbor Laboratory, 27 Oct. 2023. Crossref, https://doi.org/10.1016/j.xgen.2024.100519..., vol. 5, no. 1, American Chemical Society (ACS), 4 Nov. 2015, pp. 99–103. Crossref, https://doi.org/10.1021... Multipart Assembly.” ACS Synthetic Biology, vol. 4, no. 9, American Chemical Society (ACS), 1 May 2015...

  5. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...447–455 . https://doi.org/10.1038/s41565-019-0375-4 Lowder LG, Zhang D, Baltes NJ, Paul JW III, Tang X...

  6. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...Last updated on Oct 1, 2020 by Aliyah Weinstein. This post was contributed by guest bloggers, Wenning...blot. From the efficiency discussed above extends 4 major advantages of CRISPR mouse editing when compared...

  7. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    ...pCXLE-hUL 27080 L-MYC and LIN28 pCXLE-hOCT3/4-shp53-F 27077 OCT3/4 and shRNA against p53 pCXWB-EBNA1 ...is the expression of the reprogramming factors- OCT3/4, SOX2, KLF4, and C-MYC [1]. For the derivation ...mixture of two vectors that encoded six factors - OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen...and remains a safety concern in the clinical field [4]. Three major reprogramming methods are considered...PMID: 21243013. PubMed Central PMCID: PMC3193421. 4. Hacein-Bey-Abina, S., et al., LMO2-associated clonal...activated T cells and Sendai virus. Nat Protoc, 2012. 7(4): p. 718-28. PubMed PMID: 22422317. 6. Diecke, S.,...Mukherjee. Kusumika (Kushi) Mukherjee is currently a postdoctoral fellow at Massachusetts General Hospital with...

  8. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    ... specialized cell types [2]. We general speak of 4 levels of stem cell potency: Totipotency: When a ...zygote through to the morula stage are totipotent [2-4]. Pluripotency: When a cell has the ability to differentiate...pluripotency and nuclear reprogramming. Cell, 2008. 132(4): p. 567-82. PubMed PMID: 18295576. PubMed Central...PMID: 19343304. PubMed Central PMCID: PMC2752493. 4. Bongso, A. and M. Richards, History and perspective...fibroblast cultures by defined factors. Cell, 2006. 126(4): p. 663-76. PubMed PMID: 16904174. 9. Takahashi, ... the ectopic expression of four embryonic genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated...communication and publishing after completing her postdoctoral training from Massachusetts General Hospital...

  9. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...function in the environment where it will localize [4]. Most investigators are surprised to learn that FPs...biology."Current Protocols in Cell Biology (2005): 21-4. Pubmed PMID: 18228466. Pubmed Central PMCID: PMC2875081...Pubmed PMID: 6526011. Pubmed Central PMCID: PMC557822. 4. Costantini, Lindsey M., and Erik Lee Snapp. "Fluorescent...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz... from Albert Einstein College of Medicine. Her doctoral research was conducted in the laboratory of Erik...subcellular environment. Currently, she is a SPIRE postdoctoral fellow at the University of North Carolina at...

  10. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...only and leave exon 2 floxed, as seen in line 4 of Figure 4. Since the loxP sites are located in intronic...the targeted exon in the gene of interest (Figure 4). When recombination occurs, the gene still functions...has been placed into an intron.    Figure 4: A targeting vector to create a floxed allele for ... protein with GFP, as seen in blue flame plasmid OCT4-eGFP-PGK-Puro from the Jaenisch lab.   Figure...

  11. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ... single gene KO and pooled screening approaches; (4) encodes a Thy1.1 selectable marker to facilitate ...Framework for in vivo T cell screens. J Exp Med, 221(4), e20230699. https://doi.org/10.1084/jem.20230699.... Find FITS plasmids here! Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard...

  12. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...defined factors. Cell, 2006. 126(4): p. 663-76. PubMed PMID: 16904174. 4. Yu, J., et al., Induced pluripotent...delivery methods include retrovirus [2], lentivirus [4], adenovirus [5], Sendai virus [6], plasmid electroporation...pancreatic insulin-producing cells. Cell Res, 2009. 19(4): p. 429-38. PubMed PMID: 19255591. 17. Spence, J....converting one cell into another. Cell Stem Cell, 2008. 3(4): p. 382-8. PubMed PMID: 18940730. Additional Resources...involves overexpression of four reprogramming factors- OCT4, SOX2, KLF4, and C-MYC - that induce a differentiated...communication and publishing after completing her postdoctoral training from Massachusetts General Hospital...

  13. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...futures both inside and outside of academic research (4). There are currently 17 sites around the USA undertaking...PMID: 22567149. Pubmed Central PMCID: PMC3342243. 4. Mathur, Ambika, et al. "Transforming training to ...the Future of Research will be held from 22-24 October. This blog has been contributed by guest blogger...strong supporter of increasing the number of postdoctoral scientists at University of Maryland. Paula... can register here or live stream the event on October 22nd and 23rd here. Many thanks to our guest blogger...

  14. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...board, Jacqueline N Crawley  [et al]. 2013;4(435):4 35 1-4  20. doi: 10.1002/0471142301.ns0435s65. PMID: ...for a discussion of the different variants [1, 2, 4]. And, of course, the best way to learn about the ...animal models. Nature reviews Neuroscience. 2012;13(4):251-66. doi: 10.1038/nrn3171. PMID: 22430017. 2. ....2011.10.003.  PMID: 22055387;  PMCID: PMC3225502. 4. Mattis J, et al. Principles for applying optogenetic...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

  15. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...intermediate on the path to the formation of cysteinyl-tRNA (4). The discovery of a phosphoserine (Sep)-tRNA synthetase...pSer-incorporated proteins on a single plasmid. This includes 4 copies of a tRNA that specifically recognizes amber...letters 462.3 (1999): 302-306. PubMed PMID: 10622715. 4. Sauerwald, Anselm, et al. "RNA-dependent cysteine...contributed by guest blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute for Research...blogger Natalie Niemi! Natalie Niemi, Ph.D., is a postdoctoral fellow at the Morgridge Institute for Research...

  16. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...Cell Stem Cell 4, no. 6 (2009): 472-76. PubMed PMID:19481515. PubMed Central PMC: 2705327  4. Hockemeyer,...reprogramming à la carte." Nature Reviews Genetics 12, no. 4 (2011): 231-42. PubMed PMID: 21339765. Methods figures...PubMed PMID: 20139967. PubMed Central PMCID: 2892897. 4. Stadtfeld, M., and K. Hochedlinger. "Induced pluripotency...a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles...expression of endogenous pluripotency genes (i.e. Oct4) are other measures of reprogramming. If a retrovirus...plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check out these plasmids if...

  17. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...under a variety of conditions. They are composed of 4 proteins encoded in a single operon and were first... Find Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni,...contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela Silver.  Most types...with their environment.   Jessica Polka is a Postdoctoral Research Fellow with Pamela Silver in the department...

  18. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...your target gene! Tanenbaum et al., Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. Multi-bit genetic...Addgene. 1 Lungu et al., Chem Biol. 2012 Apr 20;19(4):507-17. 10.1016/j.chembiol.2012.02.006. 2 Guntas...expression of cDNAs and shRNAs with a reporter gene, or (4) multiple sgRNAs with Cas9 for genome engineering....Sladitschek HL and Neveu PA. PLoS One. 2015. Apr 24;10(4):e0124958. LOC8-ing mitochondrial fusion with a...system was also designed for a short screening time (<4 hours) in order to avoid complicating effects from...improving the strength of the promoters used; and 4) optimizing expression of both the light sensor and...The proteins encoded in this set range in size from 4 to 156 kDa, enabling the user to characterise the ...

  19. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...and potentiates the effect of PD-1 blockade.“ (Ref 4). PD-1 helps tumor cells evade the immune system -...356.6338 (2017): eaal3118. PubMed PMID: 28495700. 4. Zelenay, Santiago, et al. "Cyclooxygenase-dependent...Relationship” by our guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University! Cancer therapeutics...

  20. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...microenvironment produce CTL associated protein 4 (CTLA-4), a receptor that turns off T cell function upon...immunology 3.11 (2002): 991-998. PubMed PMID: 12407406. 4. Weiner, George J. "Building better monoclonal antibody-based...contributed by guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University Cancer is one...Subhadra Jayaraman! Subhadra Jayaraman is currently a doctoral candidate at Binghamton University. She works ...
Showing: 21 - 40 of 76 results