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We narrowed to 4 results for: 4-Oct

Showing: 1 - 4 of 4 results

  1. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...determine optimal antibiotic concentration. Last Update: Oct. 27, 2017 Protocol Video Watch the protocol video...Prepare carbenicillin to a concentration of 1 mg/mL – 4 mg/mL in LB medium. The concentration of antibiotic... 1 mg/mL plate and effective selection. 150 µL of 4 mg/mL Carbenicillin plated over-agar Plate shows several...

  3. AAV Production in HEK293 Cells

    Type
    Protocol
    ...needed. Add stir bar and stir slowly at 4 °C for 1 h, then keep at 4 °C for 3 h without stirring to allow...Reagent Preparation DMEM Complete : 10% v/v FBS and 4 mM L-alanyl-L-glutamine (or stable alternative, such...glutaGRO 11 mL of 200 mM L-alanyl-L-glutamine. Store at 4 °C. D1 + 0.1 M sorbitol (optional) : DMEM low glucose... °C. After thawing, the solution can be stored at 4 °C for up to 2 months. After 2 months, discard the...solution may separate into phases. Aliquot and store at 4 °C. Cell Lysis Buffer : 50 mM Tris HCl, 150 mM NaCl... Tris HCl pH 8.5 + 60 mL of 5 M Sodium Chloride + 4 mL of 1 M Magnesium Chloride Close the bottle and ...Filter sterilize through a 0.22 μm membrane. Store at 4 °C. Considerations Before You Start The health of ...

  4. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...for 2 h at room temperature or overnight at 4 °C . Section 4: Secondary antibody incubation Carefully remove... over several days by incubating certain steps at 4 °C instead of room temperature or 37 °C. The protocol...: Blocking Day 3: Primary antibody incubation Day 4: Secondary antibody incubation and plate read Tips...and incubate at 37 °C for 30 min , or overnight at 4 °C . Section 2: Block the plate Prepare the wash buffer...400 rpm for 2 h at room temperature or overnight at 4 °C . Section 3: Primary antibody incubation Carefully...400 rpm for 2 h at room temperature or overnight at 4 °C . Section 5: TMB reaction Carefully remove the ...micrograms of antigen loaded. Last reviewed on: October 8, 2024...
Showing: 1 - 4 of 4 results