...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...of signaling molecules. Select one appropriate for your application and experimental design. Will you ...transcriptomics or proteomics. This approach is often appropriate for quantitative methods. Capture MS...protein of interest, with no overlapping epitopes. It is not appropriate for validation of an antibody...production and storage techniques, and explain common applications. Science... Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods...

...binding protein 1 CREB, MGC9284 CTSB cathepsin B APPS, CPSB CTSE cathepsin E CATE CTSL1 cathepsin L1 CATL...immunoglobulin kappa joining 1 J1 IGKJ2 immunoglobulin kappa joining 2 J2 IGKJ3 immunoglobulin kappa joining ...immunoglobulin kappa joining 4 J4 IGKJ5 immunoglobulin kappa joining 5 J5 IGKV@ immunoglobulin kappa variable...IGKDEL immunoglobulin kappa deleting element or like IGKDE IGKJ@ immunoglobulin kappa joining group IGKJ ...IGKV1-12 immunoglobulin kappa variable 1-12 IGKV112, L19 IGKV1-13 immunoglobulin kappa variable 1-13 (gene...IGKV1-16 immunoglobulin kappa variable 1-16 IGKV116, L1 IGKV1-17 immunoglobulin kappa variable 1-17 A30, IGKV117...IGKV1-27 immunoglobulin kappa variable 1-27 A20, IGKV127 IGKV1-33 immunoglobulin kappa variable 1-33 IGKV133...

...sfGFP David Spray 54959 mApple-VE-Cadherin-N-10 Tight Junctions VE-Cadherin mApple Michael Davidson 55165...moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic Reticulum ER retention signal oxBFP Erik Snapp 49150 BFP-KDEL...GolgiCFP Golgi apparatus eNOS(1-33) CFP Alexandra Newton 36205 pmTurquoise2-Golgi Golgi apparatus B4GALT1(1...68073 GalToxBFP Golgi apparatus GalT signal anchor transmembrane domain oxBFP Erik Snapp 158005 pCMV-mGold-MannII-N...10 Golgi apparatus MannII mGold Francois St-Pierre 85050 pmScarlet-i_Giantin_C1 Golgi apparatus Giantin...pmScarlet-H_Giantin_C1 Golgi apparatus Giantin mScarlet-H Dorus Gadella 85048 pmScarlet_Giantin_C1 Golgi apparatus Giantin ...Giantin Golgi apparatus Giantin mTurquoise2 Dorus Gadella 98880 mNeonGreen-Giantin Golgi apparatus Giantin ...

...for your needs 5 great apps for lab life  Sharing your lab protocols using apps to save you time and track...sign-in required.)  DailyCalcs Science Calculator app for iPhone Reagent and Data Repositories These links...paste into other applications Primer design tools Many of the above-listed plasmid mapping tools have built-in...Primer design. Plasmid mapping. DNA sequence analysis. We all have our favorite tools for tackling these...molecular biology tools all in one place. Plasmid mapping These tools are for viewing, editing or making ...the paid version provides more tools for plasmid mapping and design (Figure 1). Benchling: While you might... desktop-based software for plasmid design and mapping. ApE (A plasmid Editor): A free, donation-based...

...suitable for applications where orientation of the insert is important. Type V-K CASTs appear to exhibit... (2015). A scalable Genome-Editing-Based approach for mapping multiprotein complexes in human cells. Cell...technologies. Before CRISPR, genome engineering approaches like zinc finger nucleases (ZFNs) or transcription-activator-like...rapidly became the most popular genome engineering approach. Figure 1: Overview of the basic CRISPR mechanism... to various Cas enzymes have extended CRISPR applications to increasingly complex functions, including...understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR... Cas12a, which are available for specialized applications. Check out our list of additional Cas proteins...

...Cas9 in mammalian cells, many of these principles apply to using CRISPR in other organisms. First, consider...a good way to narrow down which reagents are appropriate for a given experiment. Make sure to check whether...may not be a perfect plasmid for your specific application, and in such cases, it may be necessary to customize...reagent to suit your needs. Genetic Manipulation Application Cas9 gRNA Additional Considerations Knockout ...Cas enzymes increase specificity. Dual-nickase approach increases specificity but is less efficient. Each...viral delivery of CRISPR reagents may be more appropriate. In cases where off-target editing is a major...method Expression System Components of System Application Mammalian expression vector Cas enzyme promoter...

...therapeutic applications. Despite the ethical controversies surrounding non-research applications, it’s clear...Y. 2015. A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell...just as useful (if not more so) for research applications, eclipsing past genome engineering technologies...proteins snip foreign DNA into small fragments approximately 20 bp in length and paste them into contiguous...for CRISPR genomic editing. Genome Engineering Applications In 2012, Jinek et al. first demonstrated that...various species, as well as the development of new applications. The first CRISPR papers described two main ...RNA, making it a very flexible system. Other Applications Scientists have also used the targeting capability...

...Bacterial Expression mApple 568 592 37 6.5 30 min Monomer mApple-N1 - Mammalian Expression mApple-C1 - Mammalian...Expression mT-sapphire 399 511 Monomer (A206K) mT-Sapphire-N1 - Mammalian Expression mT-Sapphire-C1 - Mammalian...Structure Plasmids T-sapphire 399 511 26 4.9 1.3 hr Prone to dimerization pFA6a-link-yETSapphire-CaUra3 - Yeast...for this page was generated with the help of Erik Snapp . Empty Backbones for Fluorescent Protein Fusions...Mammalian Expression mApple-pBAD - Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-...Mammalian Expression mT-Sapphire-pBAD - Bacterial Expression mAmetrine 406 526 26 6 0.8 hr Monomer (A206K...

...production will take approximately 6–8 weeks. Additional steps, including request approval, your institution...requests will be approved within two business days! Ordering At your convenience After approval, the next step...facilitation for the transfer plasmid, including legal approval and an implementation letter DNA amplification...institution’s approval of the MTA, and shipping, mean that the actual time from request to delivery could be up...before we can begin processing your order. MTA Approval 1+ business days, varies by requesting organization...remain subject to the terms and conditions of the applicable transfer plasmid. We will help you gain legal...

...starting a writing group. Loop Habit Tracker app for Android  This app lets you track habits, like writing. It...reinforcement for a habit. The best part of this app is it turns your writing habit into beautiful graphs...

...antibodies. These monoclonal antibodies undergo application-specific validation and quality control by Addgene...antigen. Addgene supplies a list of recommended applications based on our in-house testing and data provided...with full experimental details in the Antibody Applications section of our product pages. We also include... an antibody’s use is not recommended for an application or species. We currently assess western blot,...scientists to further develop and refine these application lists. The plasmids we use to produce antibodies...Source Species Isotype Reactivity Recommended Applications PI Return to Top Do you have suggestions for...

...proof-of-concept targeting approach in 2014 1 (using EYFP and ChR2-EYFP as payloads). This approach has been broadly...gene or projection). Intersectional expression approaches combine multiplexed recombinases, including Cre...broadly applied using multiple recombinase-expression strategies to define cellular sub-populations of interest... Oberhuber M, Conzelmann KK, Staiger JF. 2019. Mapping Brain-Wide Afferent Inputs of Parvalbumin-Expressing... Dombeck DA, Deisseroth K, Awatramani R. 2018. Mapping projections of molecularly defined dopamine neuron...neuron subtypes using intersectional genetic approaches. Nat Neurosci. 21(9):1260-1271. PubMed (Link opens...

...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...generate due to expense and time. An alternative approach is to use synthetically produced recombinant antibodies...extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb...imaging. Learn more about antibodies and their applications in our Antibody Guide ! Cloning and Expressing...single domain antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7. ...

...robotic multidimensional directed evolution approach applied to fluorescent voltage reporters. Nat Chem...sensor's behavior in the target system. Use care when applying a biosensor in a novel context or modifying an...indicators based on mRuby (jRCaMP1a, jRCaMP1b) and mApple (jRGECO1a) (Constitutive or Cre-dependent) Sensitive... Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging. Sci Rep....NADP+ ratio or NAD+ Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine...Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range. Biochemistry...Positron (Constitutive or Cre-dependent) A general approach to engineer positive-going eFRET voltage indicators...

...expressed from plasmids. These antibodies undergo application-specific validation and consistent quality control...and provide experimental data for recommended applications. Addgene values transparency and accessibility...antibodies developed and validated for many research applications. Browse Antibodies Production Interested in ... storage techniques, and explain common applications. View Guide Need Some Help? Browse some of our...

...utility for the study of non-coding elements. This approach can be used for efficient loss-of-function studies...example, sgRNA-A’s protospacer sequence and PAM happen to fall on the bottom (Crick) strand while sgRNA-B... the presence or absence of deletion. For this approach, the internal primers described in step 2.1 may...Phosphorylated oligos may be ordered instead. For this approach, the use of T4 PNK is omitted. Anneal in a thermocycler... to performing this step to reliably obtain approximately 30 cells per 96-well plate. Include 100 μl per...

...sequence? Do these two proteins interact? What happens when I overexpress this enzyme at a particular ...cellular activity in a way that’s interesting from an applied perspective, sometimes it’s best to delete genes...bacteria of interest are transformed with the appropriate construct, grown at the permissive temperature...localization of your gene of interest once its appropriate cDNA has been fused to the MS2 binding sites ...

...females at an incidence of approximately 1:10,000. It is characterized by apparently normal development through...In females, random X inactivation results in approximately half of cells expressing wild-type MECP2 and...Tools CRISPR technology is a promising therapeutic approach for precise editing of mutations in the MECP2 ...

...advantages and disadvantages depending on the application. Firefly luciferase is considerably brighter ...luciferase-based plasmid systems for many different applications. Browse the Collection Highlights section to...respectively, for increased usefulness in in vivo applications. LuxSit-i : An artificial luciferase desined...desined de novo using deep-learning approaches. Split-Luciferase and Protein-Protein Interaction Assays Cluc-FKBP...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...bidirectional insertions and may not be suitable for applications where orientation matters. Type V-K systems ... TnsC, and TniQ). Unlike type I, type V-K CASTs appear to exhibit almost unidirectional insertions, but...