We narrowed to 290 results for: CO
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TypeBlog Post...clinical development programs in biopharma. AveXis, co-founded by Kaspar, acquired by Novartis and rebranded...the mutation (Le et al., 2019). Beam Therapeutics, co-founded by Harvard Investigators and Addgene depositors...syndrome program being pursued at Vico Therapeutics, co-founded by Gail Mandel. There are numerous technical.../10.1002/1096-8628(200022)97:2<147::aid-ajmg6>3.0.co;2-o Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge...
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7 Tips to Secure a STEAM Internship This Summer
TypeBlog Post... family members, and professional network such as co-workers, professors, and mentors. Ask for specific...economically disadvantaged populations. Roo is the co-founder of STEAMid. Rose C. St Pierre is currently...help their children thrive academically. Rose is the co-founder of STEAMid. Additional resources on the... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...editor is fused to a fast-splicing split intein and co-infected into the cell. Intein splitting removes ...1: The base editor is split into two plasmids and co-transfected into cells for reconstitution. Image ... permission. Though the lab suspected that the co-transduction by two viruses would lower editing efficiency... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog Post...clinical development programs in biopharma. AveXis, co-founded by Kaspar, acquired by Novartis and rebranded...the mutation (Le et al., 2019). Beam Therapeutics, co-founded by Harvard Investigators and Addgene depositors...syndrome program being pursued at Vico Therapeutics, co-founded by Gail Mandel. There are numerous technical.../10.1002/1096-8628(200022)97:2<147::aid-ajmg6>3.0.co;2-o Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...editor is fused to a fast-splicing split intein and co-infected into the cell. Intein splitting removes ...1: The base editor is split into two plasmids and co-transfected into cells for reconstitution. Image ... permission. Though the lab suspected that the co-transduction by two viruses would lower editing efficiency... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog Post... family members, and professional network such as co-workers, professors, and mentors. Ask for specific...economically disadvantaged populations. Roo is the co-founder of STEAMid. Rose C. St Pierre is currently...help their children thrive academically. Rose is the co-founder of STEAMid. Additional resources on the... -
CRISPR Plasmids - Zebrafish
TypeCollection...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes... -
SciComm with the Experts at Science in the News Part 2
TypeBlog Post... a graduate student at Harvard University, former Co-director of Science in the News, and member of the...received her PhD from Harvard University, is a former Co-director of Science in the News, and is a member ... -
CRISPR Plasmids - C. elegans
TypeCollection...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post... her brother Ken Fan, and her husband Benjie Chen co-founded Addgene in 2004. They worked tirelessly to...the Room or Habitat for Humanity take you and your co-workers out of the office and into a completely new...mix It’s easy to sit around and just eat with your co-workers. Don’t fall into that rut! We’ve done everything... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog Post... to study these genes! Groucho, a transcriptional co-repressor The first conference room, that is actually...named after the protein Groucho, a transcriptional co-repressor important in development. Groucho family...Ish-Horowicz. "The Groucho/TLE/Grg family of transcriptional co-repressors." Genome biology 9.1 (2008): 205. PubMed... -
Plasmids 101: Origin of Replication
TypeBlog Post... rule, plasmids from the same group should not be co-transformed, so if you need two plasmids for an experiment...from the same incompatibility group should not be co-transformed. +Actual copy number varies. See below... -
25 Work From Home Tips for Scientists
TypeBlog Post... Technique or the Flowtime Technique. Co-work virtually. With co-working, the idea is not necessarily ... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing the...first round of translation While ribosome profiling co-opts the physical location of the ribosome, TRICK... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog Post... to study these genes! Groucho, a transcriptional co-repressor The first conference room, that is actually...named after the protein Groucho, a transcriptional co-repressor important in development. Groucho family...Ish-Horowicz. "The Groucho/TLE/Grg family of transcriptional co-repressors." Genome biology 9.1 (2008): 205. PubMed... -
CRISPR Plasmids - Drosophila
TypeCollection...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...all organisms and cell types, (ii) no need to add a co-factor, (iii) easy visualization with standard filter...conditions, proteins that bind endogenous fluorescent co-factors can be used. Examples include the LOV domains...Kumagai, 2013). Next to these naturally occurring co-factor binding proteins, systems wherein a protein... -
K. phaffii: Rising to the Occasion
TypeBlog Post...for methanol usage. This gene promoter, AOX1, was co-opted for the expression of recombinant proteins ...Saccharomyces cerevisiae to human: The important gene co‑expression modules. Biomedical Reports 7.2 (2017)... -
AAV Q&A with Tim Miles
TypeBlog Post...trans-protein splicing. Note, however, that this will require co-delivery of two AAVs at a high MOI, often resulting...microglia in vivo. Q5: What is the efficiency of co-delivering different AAV particles into the same ... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...fluorescent protein tag which let the team detect its co-localization with a membrane-localized mCherry or...protein. With this system, if the OptoNB can bind, it co-localizes with its target at the cell membrane and...