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We narrowed to 184 results for: CO

Showing: 21 - 40 of 184 results

  1. Zhang Lab CRISPR Page

    Type
    Collection
    ...Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease...expressing human codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature ...human codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar...

  2. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ... her brother Ken Fan, and her husband Benjie Chen co-founded Addgene in 2004. They worked tirelessly to...the Room or Habitat for Humanity take you and your co-workers out of the office and into a completely new...mix It’s easy to sit around and just eat with your co-workers. Don’t fall into that rut! We’ve done everything...

  4. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing the...first round of translation While ribosome profiling co-opts the physical location of the ribosome, TRICK...

  6. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ... rule, plasmids from the same group should not be co-transformed, so if you need two plasmids for an experiment...from the same incompatibility group should not be co-transformed. +Actual copy number varies. See below...

  7. Brain Initiative Collection

    Type
    Collection
    ...112006-AAV9 pAAV-hSynapsin1-GCaMP6s-P2A-mKate2 For co-expression of GCaMP6s with mKate2 Lin Tian 112007...112007-AAV9 pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 For co-expression of GCaMP6s with mRuby3 Lin Tian 112010-AAV5...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. Jonathan...-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR...

  8. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...all organisms and cell types, (ii) no need to add a co-factor, (iii) easy visualization with standard filter...conditions, proteins that bind endogenous fluorescent co-factors can be used. Examples include the LOV domains...Kumagai, 2013). Next to these naturally occurring co-factor binding proteins, systems wherein a protein...

  9. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...and Women’s Hospital (BWH). Based on her experience co-directing Mentor Circle Programs at BWH and providing...is a Junior Faculty at Harvard Medical School/BWH, co-Director of the Mentor Circle Program at BWH, and...health strategy, and global health equity. She is also Co-founder/director of the non-profit foundation IMFAHE...

  10. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...for methanol usage. This gene promoter, AOX1, was co-opted for the expression of recombinant proteins ...Saccharomyces cerevisiae to human: The important gene co‑expression modules. Biomedical Reports 7.2 (2017)...

  11. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...trans-protein splicing. Note, however, that this will require co-delivery of two AAVs at a high MOI, often resulting...microglia in vivo.   Q5: What is the efficiency of co-delivering different AAV particles into the same ...

  13. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...Concentation*** Blasticidin bsd  HeLa, NIH3T3, CHO, COS-1, 293HEK Inhibits termination step of translation...termination 1-10 ug/mL Zeocin Sh bla HeLa, NIH3T3, CHO, COS-1, 293HEK, Jurkat T cells Complexes with DNA; causes...

  14. E11 Bio PRISM Collection

    Type
    Collection
    ...plasmids can be packaged as AAVs and pooled together for co-delivery. Alternatively, plasmids can be combined...

  15. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...number of new platforms (such as openUC2, which I am a co-founder of), that aim to support the community in...the Heintzmann Lab at the Leibniz IPHT Jena. He is co-founder of the open-hardware startup “openUC2” and...

  17. Antibodies 101: Isotypes

    Type
    Blog Post
    ... with its variable chain domain. B cells start by co-expressing IgM and IgD antibodies, but later in an...recombination of the antibody gene locus. B cells start by co-expressing IgM and IgD isotypes antibodies which ...

  19. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...ClonTracer library originally came about as Bhang and co-workers were trying to develop a way to quantify ...ClonTracer to study drug resistance Although Bhang and co-workers started using the tool to look at a variety...

  20. Working with Nuclear Receptors

    Type
    Blog Post
    ...steady-state complexes with secondary coregulator (or co-coregulator) partners. The coregulator ‘core’ complex...group of proteins, whereas the more loosely bound co-coregulators associate with the core complex in a...
Showing: 21 - 40 of 184 results