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...vector available to the scientific community. Please cite Moffat et al., Cell 2006 Mar; 124(6):1283-98 (PubMed... on the size of your target plate. TIP: MOI (multiplicity of infection) refers to the number of infecting... media 24 hours after infection. TIP: If viral toxicity is observed in your cell line, you may decrease...

...switch system takes advantage of the orientation specificity of Cre and the different types of target sites...target site cassette in a double-crossover event. Toxicity Cre-lox recombination is a very useful tool, but...such as Cre-only lines to account for cellular toxicity in your system. Using inducible Cre systems in...-target events and the systemic effects of Cre toxicity. Leakiness Leaky expression, where the gene of...number of recombination events but can improve specificity in targeted neuronal populations. Read our blog...

...many of which detail work to improve the tool’s specificity, orthogonality, and multiplexibility in various...endogenous loci with unprecedented speed and specificity. Figure 2: An overview of CRISPR and NHEJ/HDR...Joung JK. 2014. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol . ...Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature . 523(7561):481-5. PMID: 26098369 Komor...Rationally engineered Cas9 nucleases with improved specificity. Science . 351(6268):84-8. PMID: 26628643 Wang...

... to recognize their targets with fairly high specificity. Fifteen years of research has shown that when...zinc fingers within an array can reduce their specificity. Two years ago, groups led by Jens Boch at the...though NN can also bind adenenine with lower specificity). With the template for studying this type of...likelihood of context having a negative effect on specificity is on par with ZF arrays, if not slightly lower...

...developed, including additional fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium...Flp AND NOT Cre Yes Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations...neuron glutamate cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons...Andrási T, Deisseroth K, Holmes A, Hájos N. 2019. Excitation of diverse classes of cholecystokinin interneurons...

...MGC126648 CALCB calcitonin-related polypeptide beta CALC2, CGRP-II, CGRP2, FLJ30166 CALCR calcitonin receptor...MLT1 NFAT5 nuclear factor of activated T-cells 5, tonicity-responsive KIAA0827, NF-AT5, NFATL1, NFATZ, OREBP...component 5a receptor 1 C5A, C5AR, C5R1, CD88 CALCA calcitonin-related polypeptide alpha CALC1, CGRP, CGRP-I...receptor CRT, CTR, CTR1 CALCRL calcitonin receptor-like CGRPR, CRLR CAMP cathelicidin antimicrobial peptide ...somatostatin receptor 5 - ST2 suppression of tumorigenicity 2 - STC1 stanniocalcin 1 STC STC2 stanniocalcin...interleukin 9 receptor CD129 ST2 suppression of tumorigenicity 2 - TXLNA taxilin alpha DKFZp451J0118, IL14...polypeptide-related sequence B PERB11.2 NCR1 natural cytotoxicity triggering receptor 1 CD335, FLJ99094, LY94,...

...are proliferative, unspecialized cells with the capacity to differentiate into many different cell types...every other cell type in the body and have the capacity to give rise to an entire organism. iPSC technology...which lower the risk of mutation and minimize tumorigenicity, an inherent disadvantage of iPS cell technology...Neurons Lentiviral Human Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor ...

...imaging tools in this blog post . Far-Red Protein Excitation (nm) Emission (nm) Brightness Plasmids mCardinal...Crimson pAAV-CAG-E2-Crimson Near-Infrared Protein Excitation (nm) Emission (nm) Brightness Plasmids emiRFP670... 720 5.8 piRFP720-N1 Photoactivatable Protein Excitation (nm) Emission (nm) Activation (nm) Brightness...

...High-fidelity Cas enzymes increase specificity Dual-nickase approach increases specificity but is less efficient Each...be able to completely avoid them. To increase specificity, consider using an engineered Cas enzyme with...use in the lab, but AAVs exhibits the lowest immunogenicity and are one of the least toxic methods for ...

... recombinant monoclonal antibodies with high specificity for critical axon guidance signaling proteins...neurotransmission, synapse formation, neural connectivity, plasticity and neurological disorders. The initial collection...Integrin antibodies exhibit distinct binding specificities — recognizing individual α or β subunits, or...

...Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden Citation Information When using the Caltech serotypes in...publications, please acknowledge Viviana Gradinaru and cite as follows: PHP.eB, PHP.S: Chan et al., 2017 Pubmed...L4 - AAV.CAP-B10 with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For...

...non-coding RNA elements capable of programming the specificity of the CRISPR-mediated nucleic acid cleavage....10.1126/science.1231143. PubMed . DNA targeting specificity of RNA-guided Cas9 nucleases. Hsu PD, Scott DA...RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Ran FA, Hsu PD, Lin CY, Gootenberg JS, Konermann...

...in the 2009 Joung Nature Protocols paper . How to Cite this Kit These plasmids were created by your colleagues.... Please acknowledge the Principal Investigator, cite the article in which they were created, and include...

...hybridoma-derived equivalent to ensure that potency and specificity have been retained. If the target antigen is ...pattern is compared to the mRNA expression pattern cited in the Tissue Atlas (Link opens in a new window)...

...behavior and emotion, uncontrolled movements, memory deficits, as well as difficulty speaking and swallowing...cause of dementia. Symptoms include memory loss, deficits in decision-making, and language abilities. The...

...Sciences has screened thousands of enhancers for specificity and brightness of fluorescent labeling across...collection includes the top vectors with the highest specificity and brightness, assessed using ssRNAseq or high-resolution...

... VP16 transcriptional activation domain). For simplicity, other promoter elements and transcription machinery...and tTA. Dox has good tissue distribution, low toxicity, a known half-life (24 hours), and is relatively...

...bath, and be sure to know what the maximum fill capacity is. Look for a “fill” line inside the water bath...

...the first G on 3'-5' strand from cut site). For simplicity, only the 5' primer is shown here. Note: Use ...

... lentiviral vector allows you to control the multiplicity of infection (MOI) in downstream studies. This...