...AAV Production 6–8 weeks Quality is our priority. During this time, we amplify DNA, produce the prep, and...

...amplify a portion of the intended lentiviral insert. During this cultivation, cells are routinely checked for...

...1, which may require additional lab biosafety procedures. Additionally, because this virus integrates ...

...mid-sixties, or late-onset, where symptoms appear during or after a person's mid-sixties. The early-onset...

...conventional HDR-mediated gene knock-in. The detailed procedure can be found in Sakuma et al., Nature Protocols...

...sites are called off-targets and should be examined during gRNA design. In general, off-target sites are not...

...gene.. TREAT (3( T hree)′- R NA E nd A ccumulation during T urnover) : Single-molecule mRNA turnover biosensor...

...interrogation and monitoring of brain cells and circuits during behavior. Long desired by researchers, the revolutionary...

...Editing in Monocot and Dicot Plants Plant PCR-based procedure to rapidly generate multiple sgRNA expression ...

...valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary immune response; found mostly in blood...

...recommend using the HEK-2936E cell line engineered by Durocher et al., 2002 (Link opens in a new window) . The...76. PMID: 20525357 (Link opens in a new window) Durocher Y, et al. 2002. High-level and high-throughput...

...coli , deleting the floxed portion of a construct during the cloning process). VCre and SCre are alternative...

...like to go to. During the conference Take notes during talks. If you have questions during the talk, jot...conference depends on what you put into it before, during, and after the meeting. Let’s break it down into...Try to avoid jargon in this initial introduction. During the conference When you meet someone new at a conference...poster) so your visitors can have a DOI to go to. During the conference Arrive at the poster session early...the board and meet your poster session neighbors. During the session, if someone comes up to my poster, ...discussing with your lab. If you made a connection during the poster session, send them a follow up email...to turn to an established social media platform. During the conference If the conference has a social media...

...worry about traveling during an already stressful time, and I was more relaxed during both sets of interviews...yourself to a few members of your desired department during the application process. Many institutions recommend...in case one isn’t able to respond to your email. During my PhD application cycle, I reached out to around...faculty that you might be working with for the duration of your program and ask any questions that will... and make sure you cover everything you need to during the interview. (Virtual) Campus Visits After receiving...Through the virtual systems created and promoted during COVID-19, online meetings with current students...worked with students to maintain these time goals during COVID-19? How are students funded? Are there any...

...role was incredibly valuable because it was a set duration of only three months and made a good job to list...list on my CV. Three months was a perfect duration for me because it was sufficient to figure out if my...problem solving. I believe that is something lacking during degree courses; they never really prepare you for...failure and problem solving scientists experience during research. Working abroad in rural Africa Once this...another area which I was really passionate about during my degree. I loved parasitology. However, when ... held me back; I do not have a second language. During the recession there were many qualified scientists... controlled. I worked from Standard Operating Procedures (SOP’s) and every item I tested was constantly...

...data for lab procedures. With this data in hand, we can go back and optimize our procedure to make the ... biology is messy. Every single step in a lab procedure can and will fail. Some step failures require ...monumentally difficult without software. Tracking lab procedures also gives you access to hard data about what...definitely be interested to see which part of her procedures are the most failure prone. In an academic lab... Some steps fail “hard”, they reset the whole procedure. Other steps fail “soft” they simply need to be...might leave you sad and confused when a tricky procedure failed and one of your techs started over with... but the software should be mirroring the lab procedures, not artificially constraining them). In the ...

... if we were to restart the procedure we would not have enough time. During the following two weeks, we...summer interns presenting their capstone projects during the Summer Intern Poster Presentation.  As ...the Syntaxin3 gene in a pLenti vector backbone. During those five weeks, I worked with my mentors, Katherine...always came with surprises. Originally we thought during the first three weeks that we had made a mistake...not having the right concentration of a reagent during our HiFi assembly but decided to go ahead anyway...on my capstone project report and presentation, during the final week at Addgene. This report shared my...

...As Addgene believes many of the topics discussed during the symposium will prove incredibly important to... that the main question they wanted to focus on during the symposium was “How many graduate students/post-docs...problems in academic research and potential solutions During the first couple of sessions of the symposium it...not easily achieved. Of note, it was highlighted during the conference that just increasing funding for...Editors I wish I had been more cognizant of this during my PhD. Submitting a publication doesn’t have to...symposium was on diversity in academic research. As during previous panels, speakers Moon Duchin, Rafael Luna...researchers and, in fact, one of the projects developed during the Saturday Hack Day Session focused on finding...

...specific subset of cells in a population in a procedure termed fluorescence activated cell sorting or ...specialized components that can divert a subset of cells. During FACS, the sample stream oscillates to generate ...be targeted with specific fluorescent antibodies during the flow cytometry experiment and will appear as...factors including sample preparation, staining procedures, and controls. To prevent instrument clogs, ensure...control with cells that went through the staining procedure minus the antibody. An unstained control allows...of yellow and orange from FITC and that from PE. During compensation the signal arising from a specific...fluorescence-minus one panel will help you set up gating during data analysis.  We hope that this blog has provided...

...packaged inside the vectors during production. These impurities cannot be removed during the purification step...recombinase-independent recombination during plasmid growth in bacteria or during viral production. This recombination...wrong plasmid? What if bacteria snuck into the prep during the production process? These questions are especially...Evidence for Encapsidation of Prokaryotic Sequences during Recombinant Adeno-Associated Virus Production and...