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We narrowed to 72 results for: HDR

Showing: 21 - 40 of 72 results

  2. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...improvement over Cas9-mediated HDR for the loci tested, where average HDR-mediated editing frequency was... editing efficiency of homology-directed repair (HDR), the pathway for resolving DNA double-strand breaks.... Compared to non-homologous end joining (NHEJ), HDR occurs at a relatively low frequency, especially ...breaking the DNA backbone, sidestepping the problem of HDR vs. NHEJ entirely. The first two classes of base ...

  3. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...homology-directed repair (HDR) to correct double-strand breaks (DSBs). DSB repair by HDR facilitates in-frame... or HA). Tag-specific PCR genotyping can tell if HDR completely failed. If C-terminus tagging potentially...damage (indels) to the promoter region of the non-HDR allele. For N-terminal tagging, specifically searching...

  4. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...through exploiting the homology-directed repair (HDR) pathway which occurs in cells at low frequencies... 25-75%, while the success of precise change via HDR limited to 0-5%. This blog post covers a brief review...genome editing in rice and uses base editors and HDR methods for rice crop improvement in the laboratory...

  5. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...homology-directed repair Homology-directed repair (HDR) stimulated by double strand breaks has been widely...cleavage is relatively high while the efficiency of HDR is relatively low, meaning that most Cas9-induced...most products to be indels while the efficiency of HDR is typically less than 10%. In contrast, the original...desired edits to indel byproducts than Cas9-initiated HDR. Prime editing tools Prime editing is precise and...

  6. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...the host cell’s inherent homology directed repair (HDR) process following the double strand break CRISPR...PITCh system plasmids provides an alternative to HDR as the double-strand break repair pathway, relying...

  8. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...types, gene editing using homology directed repair (HDR), and generating large genomic deletions. What are...et al. 2016). For CRISPR experiments that involve HDR, electroporation can be combined with cell-type specific...

  10. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ... with other large insert techniques like HITI or HDR, which require specific repair pathways to work, ...has higher purity of insertion than both HITI and HDR. PASTE is also compatible with both AAV and adenovirus...

  11. Plan Your Experiment

    Type
    Guide
    ...targeting the region where the edit should be made HDR requires a repair template and displays reduced efficiency...protein domains. For gene editing experiments using HDR, it is essential that the target sequence be very...different owing to the error-prone nature of NHEJ. In HDR gene editing experiments, most mutated alleles will...identify DNA fragments. PCR and restriction digest (for HDR repaired DSBs): For small nucleotide edits that introduce.... PCR amplification and gel electrophoresis (for HDR or NHEJ): For large deletions or insertions, the ...amplification, subcloning and Sanger sequencing (for HDR or NHEJ): Provides semi-quantitative assessment of...amplification and next-generation sequencing (for HDR or NHEJ): Provides quantitative assessment of the...

  12. Plan Your Experiment

    Type
    Collection
    ...targeting the region where the edit should be made HDR requires a repair template and displays reduced efficiency...protein domains. For gene editing experiments using HDR, it is essential that the target sequence be very...different owing to the error-prone nature of NHEJ. In HDR gene editing experiments, most mutated alleles will...identify DNA fragments. PCR and restriction digest (for HDR repaired DSBs): For small nucleotide edits that introduce.... PCR amplification and gel electrophoresis (for HDR or NHEJ): For large deletions or insertions, the ...amplification, subcloning and Sanger sequencing (for HDR or NHEJ): Provides semi-quantitative assessment of...amplification and next-generation sequencing (for HDR or NHEJ): Provides quantitative assessment of the...

  14. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...genome via the homology directed repair pathway (HDR), be it a single nucleotide change, insertion of ...mutations by NHEJ and generating small mutations with HDR, when it comes to larger scale genome editing, such...

  15. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower ...introduce specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication ...

  16. CRISPR Guide

    Type
    Guide
    ...Modifications Using Homology Directed Repair (HDR) Figure 4: Overview of HDR HDR generates specific genomic edits ...but high-fidelity homology directed repair (HDR) pathway. HDR relies on copying DNA from a matching template... Both NHEJ and HDR have their uses, but we’ll focus on NHEJ first and come back to HDR . NHEJ is a naturally...that modulate HDR or NHEJ components, like miCas9 , Cas9-DN1S , or others, can increase HDR efficiency. ...base. These small precision edits can be done via HDR, base editing, and prime editing, and are often used...mutation that does not change the amino acid sequence. HDR efficiency is generally low (< 10% of modified alleles...Synchronizing cells or modulating the cell cycle can help as HDR takes place during the S and G2 phases. Chemically...

  17. CRISPR Plasmids - Yeast

    Type
    Collection
    ...homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower ...introduce specific edits via homology-directed repair (HDR). ID Plasmid Description Gene/Insert Promoter Selectable...

  19. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...repair the break using homology-directed repair (HDR). HDR is much less error-prone than NHEJ and can be ...

  20. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower ...
Showing: 21 - 40 of 72 results