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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 663 results for: PPL;

Showing: 21 - 40 of 663 results

  1. CRISPR Guide

    Type
    Collection
    ... to various Cas enzymes have extended CRISPR applications to increasingly complex functions, including...understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR... Cas12a, which are available for specialized applications. Check out our list of additional Cas proteins...genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using...to help you select an optimized gRNA for your application (see: Plan Your Experiment ). In addition to ...edits, they can be used for virtually any CRISPR application. Cas9 Orthologs While SpCas9 is the most commonly...require longer homology arms. Depending on the application, the donor DNA may be a single-stranded oligonucleotide...

  2. Centrifugation

    Type
    Protocol
    ...according to speed and time (and temperature, if applicable) listed in your protocol. Pro-Tip Spin speed ...

  3. Fluorescence Titering Assay

    Type
    Protocol
    ... these cells can still be used in downstream applications. Safety Warnings Lentivirus is generally considered...

  6. Immunocytochemistry

    Type
    Protocol
    ...as methanol or acetone may be better for some applications. Remove the paraformaldehyde and follow your...

  7. Lentivirus Production

    Type
    Protocol
    ...lentivirus can be used for a variety of downstream applications such as stable-cell line generation. Workflow...

  8. Antibodies

    Type
    Collection
    ...expressed from plasmids. These antibodies undergo application-specific validation and consistent quality control...and provide experimental data for recommended applications. Addgene values transparency and accessibility...antibodies developed and validated for many research applications. Browse Antibodies Production Interested in ... storage techniques, and explain common applications. View Guide Need Some Help? Browse some of our...

  9. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information New England Biolabs offers non-toxic...Single constructs are compatible with multiple applications (different fluorophores, biotin, bead purification...MCP-tag Control for cell surface localization Supplemental Information SNAP and CLIP tags SNAP and CLIP...purchasing coenzyme A disodium salt from a commercial supplier and conjugating it to the label of their choice...

  11. Microbiology Resources

    Type
    Collection
    ...our Search page to look for specific genes or applications that are not listed here. Addgene Microbiology... cloning and expression systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors...Densmore Lab EcoFlex MoClo : Modular cloning for applications like recombinant protein purification and cell-free...fluorescent protein plasmid collection is organized by application and by color. External Resources European Saccharomyces...

  13. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Bacterial Expression mApple 568 592 37 6.5 30 min Monomer mApple-N1 - Mammalian Expression mApple-C1 - Mammalian...Mammalian Expression mApple-pBAD - Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-...and emission maxima (Stokes shift), useful for applications like multicolor imaging with better spectral...

  14. Luciferase Plasmid Collection

    Type
    Collection
    ...advantages and disadvantages depending on the application, and different luciferases require different ... course experiments, but is too dim for some applications. NanoLuc® is small (19 kDa), very bright, and... available, making it ideal for a variety of applications. To learn more and plan your experiment, check...OgNLuc ) fusions for BRET-based imaging and other applications. And see PalmGRET , a membrane-anchored version...

  15. Tetracycline Inducible Expression

    Type
    Collection
    ...tTA-Advanced Takeshi Imai Other Tet Applications Explore some other popular applications of tetracycline systems ...for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative Doxycycline...vectors for transactivators, and vectors for other applications, or search our collection for all dox-regulated...

  16. Institute for Protein Innovation

    Type
    Collection
    ...Antibody Description Isotype Reactivity Recommended Applications Integrin Antibodies Integrins are a family of...Antibody Description Isotype Reactivity Recommended Applications Browse Epitope Tag Antibodies IPI has developed...Antibody Description Isotype Reactivity Recommended Applications Browse Antibody Plasmids The following plasmids...

  17. Antibody Plasmid Collection

    Type
    Collection
    ...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb...imaging. Learn more about antibodies and their applications in our Antibody Guide ! Cloning and Expressing...

  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...sensor's behavior in the target system. Use care when applying a biosensor in a novel context or modifying an...indicators based on mRuby (jRCaMP1a, jRCaMP1b) and mApple (jRGECO1a) (Constitutive or Cre-dependent) Sensitive... Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging. Sci Rep....Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range. Biochemistry...robotic multidimensional directed evolution approach applied to fluorescent voltage reporters. Nat Chem Biol...

  20. CRISPR Library Amplification

    Type
    Protocol
    ...sufficient quantities of library for experimental applications. Repeated amplifications should be avoided as...
Showing: 21 - 40 of 663 results