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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 588 results for: Ret;

Showing: 21 - 40 of 588 results

  1. Using a Light Microscope Protocol

    Type
    Protocol
    ...resolution (the ability to distinguish between two discrete objects). Figure 1 depicts an image of a compound... sample information so that you or others can interpret your images in the future. Conclusion Like any...

  2. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...results in cell populations that are more likely to retain stable transgene expression. Other methods of generating...well in sparse/individual cultures due to lack of secreted growth factors, so not every cell type will be...

  3. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...position 15-19. Avoid targeting introns. Avoid stretches of 4 or more nucleotide repeats, especially repeated...Incubate for 20-30 minutes at room temperature. g. Retrieve HEK-293T cells from incubator. The cells should...

  4. Protocol - How to Design Primers

    Type
    Protocol
    ...end, and the 5’ end of the primer usually has stretches of several nucleotides. Also, both of the 3’ ends...

  6. Ligation Independent Cloning

    Type
    Protocol
    ...cloning scars"). A “stuffer” sequence allows for electrophoretic separation of linearized vector from the reaction...

  8. General Transfection

    Type
    Protocol
    ...first. Pro-Tip The pH of this solution will drift pretty rapidly upon addition of acid or base. Add only...

  12. Pouring LB Agar Plates

    Type
    Protocol
    ...manufacturer to make sure this is true in your case. Retrieve your molten agar mix from the autoclave. Pro-Tip...

  15. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Takeharu Nagai Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean/Citrine MagFRET: The First Genetically...Campbell Zinc FRET-based zinc sensor for imaging in live cells (CALWY) Genetically encoded FRET sensors to...-2 Zn2+ FRET sensor, cytosolic or ER-targeted eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for...Cytosolic or Nuclear zinc-binding FRET biosensor (Zap) New alternately colored FRET sensors for simultaneous monitoring...cyclic AMP) FRET sensors of cAMP levels for imaging in live cells Fourth-generation epac-based FRET sensors... for Imaging Insulin Secretion A Ratiometric Sensor for Imaging Insulin Secretion in Single beta Cells...Pyruvate Pyronic FRET sensor for pyruvate Imaging Mitochondrial Flux in Single Cells with a FRET Sensor for ...

  16. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Collection . Return to top Secretory Pathway The secretory pathway is named for its role in secreting proteins...Endoplasmic Reticulum ER retention signal moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic Reticulum ER retention...38770 pEF.myc.ER-E2-Crimson Endoplasmic Reticulum ER retention signal E2-Crimson Benjamin Glick 36204 ...36204 pmTurquoise2-ER Endoplasmic Reticulum ER retention signal mTurquoise2 Dorus Gadella 137804 ER-mNeonGreen...Endoplasmic Reticulum KDEL sequence mNeonGreen Dorus Gadella 137805 ER-mScarletI Endoplasmic Reticulum KDEL ...Endoplasmic Reticulum BiP ER signal sequence BFP Gia Voeltz 61807 Rtn4a-GFP Endoplasmic Reticulum Rtn4a AcGFP...Endoplasmic Reticulum Sec61 mCherry Gia Voeltz 49154 BFP-Sec61 beta Endoplasmic Reticulum Sec61 BFP Gia...

  17. Bacterial Expression Systems

    Type
    Collection
    ...Protein Timers Return to top Protein Interactions Förster resonance energy transfer (FRET) and bimolecular...tdTomato-pBAD mAmetrine1.1 (donor) tdTomato (acceptor) FRET/Dual FRET Robert Campbell Michael Davidson , Nathan Shaner...mCitrine-pBAD mTFP1 (donor) mCitrine (acceptor) FRET/Dual FRET Robert Campbell , Michael Davidson 54571 54856...mRuby2 (acceptor) FRET Michael Davidson 18856 pGWF1 ECFP (donor) Venus (acceptor) FRET Wolf Frommer 65616...Citrine (acceptor) FRET Wolf Frommer 65617 pFLIP42 mCerulean (donor) Citrine (acceptor) FRET Wolf Frommer 65618...expression upon protein–protein interaction. The cpFRET - FRET-based Biosensors Kit (Addgene #1000000021) is...Expression Protein Expression Epitope Tags Protein Tags Return to top Protein Visualization To determine the location...

  18. Immunology Research Plasmids and Resources

    Type
    Collection
    ...PSPS1, PTP, REG RETN resistin ADSF, FIZZ3, MGC126603, MGC126609, RETN1, RSTN, XCP1 RETNLB resistin like ...ULBP4, bA350J20.7 RAET1G retinoic acid early transcript 1G ULBP5 RAET1L retinoic acid early transcript 1L... 3 RAET1N Return to top BCR Signaling B cells mediate humoral immunity through the secretion of antibodies...chromogranin A (parathyroid secretory protein 1) CGA CHGB chromogranin B (secretogranin 1) SCG1 CKLF chemokine-like...FMRFAL NPPA natriuretic peptide precursor A ANF, ANP, ATFB6, CDD-ANF, PND NPPB natriuretic peptide precursor...precursor B BNP NPPC natriuretic peptide precursor C CNP NPR1 natriuretic peptide receptor A/guanylate ...cyclase A (atrionatriuretic peptide receptor A) ANPRA, ANPa, GUC2A, GUCY2A, NPRA NPR3 natriuretic peptide...

  19. Luciferase Plasmid Collection

    Type
    Collection
    ...Bioluminescence Resonance Energy Transfer (BRET) Similar to FRET, energy transfer from the luciferase reaction...Antares ) for in vivo imaging. NanoBRET : Constructs using NanoLuc® as a BRET energy donor and HaloTag labeled...OgNLuc ) fusions for BRET-based imaging and other applications. And see PalmGRET , a membrane-anchored...Kit , and G-CASE : BRET-based sensors for reporting on GPCR activity. AIMTOR : BRET biosensor for live...kDa and 36 kDa, respectively). And they are not secreted, so cells must be lysed or the substrate must ...to measure luminescence. Gaussia luciferase is secreted and more stable, enabling time course experiments... kDa), very bright, and ATP-independent, and a secreted version of the protein is available, making it...

  20. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...displays enhanced transduction of the retina following subretinal administration and enhanced transduction...transduction and lateral spread in the retina following subretinal injection. It was identified by John ...transduction and lateral spread in the retina following subretinal injection as compared to the parental...delivered directly to the brain parenchyma or subretinal space Serotypes The following serotypes were ...demonstrated to have enhanced transduction of the retina following intravitreal injection in mice and marmosets...trpYF) serotype shows enhanced transduction of the retina following intravitreal injection in mice and macaque...2009. High-efficiency transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Mol Ther...
Showing: 21 - 40 of 588 results