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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 164 results for: Stom

Showing: 21 - 40 of 164 results

  1. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...part of the recognition sequence, they can be customized to direct assembly of DNA fragments. When designed...utilize the BsaI and BsmBI Type IIS sites such that custom TAL arrays could be constructed quickly and efficiently...Nov 5. PubMed. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for...

  3. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ... as HIV, murine leukemia virus, and vesicular stomatitis virus G) are packaged as replication-defective...leukemia-based retroviral particles Vesicular stomatitis virus particles See Addgene's Viral Plasmids ...

  4. Bioinformatics at Addgene

    Type
    Blog Post
    ...chose needed to fulfill certain requirements: Customizable to our specific needs Modern and well-supported...the order and usage of the scripts needs to be customized in the pipeline for the specific needs of the...Since each process can use a unique container, we customized each container for its particular purpose and...

  5. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...one-pot reaction. Using this system, you can create custom RNA binding proteins in as little as 3 days. Abil...Abil et al., 2014 show that you can fuse custom PUF proteins to a translational repressor to down-regulate...Rajarshi, et al. "Engineering RNA endonucleases with customized sequence specificities." Nature communications...

  6. Ras Pathway

    Type
    Collection
    ...oncogene homolog Muscle RAS oncogene homolog Neuroblastoma RAS viral (v-ras) oncogene homolog RASA RASA1...RASSF10 Ras association domain family member RB1 Retinoblastoma 1 RCE1 Ras converting CAAX endopeptidase 1 ...

  7. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...IP and ChIP assays. Tag 96 genes at a time using Custom Synthetic cr:tracrRNA/Cas9 Ribonucleoprotein (csRNP...first step of optimization, we started off by using custom synthetic two-part cr/tracrRNAs for CRISPR experiments... hands, delivering Cas9 protein in complex with custom synthetic cr:tracrRNAs (csRNP) is the most efficient...

  8. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...creating flanking overhangs. These overhangs can be customized to link together multiple fragments, allowing...1x4; for 6 gRNAs, you’d use pX330A-1x6. This customization means you don’t ever need to clone in filler...plasmids allow expression of 2-5 gRNAs in zebrafish. Custom destination vectors are used depending upon the...

  9. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...the research community. Customers have taken advantage of the customizability, choosing the cargo, serotype...

  10. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...require a bachelor’s degree if you have a PhD. 4) Customize cover letters and even resumes The cover letter...project managment) your resume should also be customized to highlight different skills and to get the ...

  16. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ..., and a modified gRNA to direct a variety of customizable functions to your DNA sequence of interest. ... how the Casilio system works and how you can customize it for your experimental needs. Note: Casilio ...

  17. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...different cellular environments such as retina or glioblastoma). They also found that exo-AAVs were more resistant...brain tumor stromal cells in a mouse model of glioblastoma (Volak et al. 2018). The structure of exosome...

  19. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...sequence viewer of choice. If the depositor provided a custom annotated GenBank file, this can be found on the...BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using...

  20. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...General envelope plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however, may be used across...Element Purpose Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope ...
Showing: 21 - 40 of 164 results