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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 506 results for: Trac;

Showing: 21 - 40 of 506 results

  1. AAV Molecular Tools

    Type
    Collection
    ...Overexpression Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible tools...tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible expression...Viral Vector Packaging Service: Molecular Tools Tetracycline Transactivators Affinity Purification Neurophysiology...Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression...

  2. Promega Plasmid Collection

    Type
    Collection
    ...NanoLuc fusions are useful for both intracellular and extracellular applications where you want minimal...HaloTag, SmBiT, and LgBiT, useful for detecting and tracking key drug target proteins. Depositor...analysis and protein fusion vectors for detecting and tracking proteins using tags such as NanoLuc, HaloTag, ...signaling pathways, investigating RNA regulation, tracking protein degradation, analyzing CRISPR-mediated...measured by competitive displacement of a NanoBRET tracer, reversibly bound to a NanoLuc luciferase-fusion...

  3. Zebrafish Plasmid Collection

    Type
    Collection
    ...- Tatjana Sauka-Spengler Lab Lineage Tracing Cell lineage tracing in multicellular systems typically relies...labeling with fluorescent proteins to visualize and track clones of cells over time. Examples of useful tools...Zebrafish: A Transgenic Resource for In Vivo Lineage Tracing during Development and Regeneration - Periklis ...Lab Multibow: digital spectral barcodes for cell tracing - Sean Megason Lab Zebrabow - Albert Pan and Alex...fluorescent labeling in zebrafish Whole organism lineage tracing by combinatorial and cumulative genome editing ...

  4. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... Anti-Pan-Neurofascin (extracellular) [A12/18R] Pan-Neurofascin (extracellular) Rat Mouse IgG2a 177439...Anti-Kv1.4 K+ channel (extracellular) [L71/5R] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 177489...Mouse IgG2a 177564 Anti-ADAM22 (extracellular) [N57/2R] ADAM22 (extracellular) Mouse Mouse IgG2a 177565 Anti-GluN2B...Anti-Kv1.4 K+ channel (extracellular) [L71/22] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 190292...-Neurofascin (extracellular) scFv [A12/18] A12/18 scFv 2t Pan-Neurofascin (extracellular) Rat Mouse 190486...Kv1.4 K+ channel (extracellular) scFv [L71/5] L71/5 scFv Kv1.4 K+ channel (extracellular) Rat Mouse 190518... Mouse 190537 LRP4 (extracellular) scFv [N207/27] N207/27 scFv LRP4 (extracellular) Mouse Mouse 190538...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...trans-activating crRNA (tracrRNA) with homology to the short palindromic repeat. The tracrRNA helps recruit RNAse...together separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual...individual, unique crRNA. (4) Each crRNA:tracrRNA:Cas9 complex seeks out the DNA sequence complementary...cleaves both strands near the PAM. (6) The crRNA:tracrRNA:Cas9 complex unbinds after the double-strand break...

  6. Antibody Guide

    Type
    Collection
    ...samples to extract proteins. Homogenize (tissues only), lyse, and then collect protein extract by centrifugation...and therefore should be kept at 4 °C. Record and track lot numbers for all antibodies used in the lab. ...application Process tissue or cell culture samples to extract proteins. Homogenize (tissues only) and lyse. Cross-link...In IHC, tissue sections are assayed and the extracellular matrix remains intact. The signaling molecule...enzyme. In ICC, cells have most or all of their extracellular matrix removed. Again, the signaling molecule...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...be lysed or the substrate must be delivered intracellularly to measure luminescence. Gaussia luciferase...membrane-anchored version useful for labeling extracellular vesicles. LumiScarlet : A fusion of LumiLuc ...serve as sensors of proteome stress. pHLuc : An extracellular pH sensor consisting of a pH-sensitive GFP (...OFP (Antares), and Nanoluc®. CalFlux VTN : An intracellular calcium sensor created by fusing NanoLuc® and...

  8. Cancer Research Plasmids and Resources

    Type
    Collection
    ...mutations. Resources New Tool for Lineage Tracing: The ClonTracer Library (Frank Stegmeier) Tackling Cancers...cDNAs for studying oncogenic signaling pathways. ClonTracer Library : Barcoding library from the Stegmeier...

  9. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... Dorus Gadella 58473 pEGFP-C1 F-tractin-EGFP Actin Filaments F-tractin EGFP Dyche Mullins 41147 pEGFP ...Caveolae Cav1 mCherry Ari Helenius 37533 cfSGFP2-N Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1... in secreting proteins from the cell to the extracellular environment. Proteins are first translated at...

  10. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Optogenetics Plasmid Collection Monosynaptic Neuronal Tracing CRISPR Plasmids and Resources Site-specific recombinase...only activated in the presence of tamoxifen. A tetracycline-regulated or other drug-inducible approach may...Reporter plasmids can also be useful for lineage tracing, evaluating infection/transfection efficiency, ...

  11. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Find more Tet-inducible empty backbones on our Tetracycline (Tet) Inducible Expression page Bacteria Lac..., secretion, and display of proteins on the extracellular surface of S. cerevisiae cells His-tagged versions...but do NOT integrate into host genome pAdTrack-CMV - Shuttle vector for transgene...

  12. Zhang Lab CRISPR Page

    Type
    Collection
    ...CRISPR RNA (crRNA) and the trans-activating crRNA (tracrRNA; Hsu et al ., Nature Biotechnology 2013). We also... cassettes to express the CRISPR RNA array and tracrRNA separately (Cong et al ., Science 2013). Additionally...or SpCas9n, D10A nickase) + CRISPR RNA array + tracrRNA: This plasmid contains three expression cassettes...

  13. CRISPR Guide

    Type
    Collection
    ...dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and RNA tracking in living...editing can specify a single mutation, even within a tract of identical bases. Browse Plasmids: Prime Edit ...proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal RNA...synthetic fusion of the endogenous bacterial crRNA and tracrRNA that provides both targeting specificity and scaffolding...

  14. AAV Viral Preps

    Type
    Collection
    ...Spagehetti Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell...Cell Ablation, CRISPR Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing Samples...

  15. Antibody Production

    Type
    Collection
    ... window) . Western Blotting (WB) Proteins are extracted from cells, separated by SDS-PAGE, transferred...plasmid from transfection. This residual plasmid is extracted and amplified with primers targeting the heavy...

  16. Viral Production

    Type
    Collection
    ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...vector, incubated for 72 h, and genomic DNA is extracted. Primers and probes targeting integrated copies...

  17. Lentivirus Plasmids

    Type
    Collection
    ... ORF expression, multicistronic design enables tracking of viral transduction. Also see plasmid 46948 ...inducible knockdown, multicistronic design enables tracking of viral transduction. See article for more similar...

  18. CRISPR References and Information

    Type
    Collection
    ...and BsmBI site for cloning in gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF, 49 KB Vosshall...RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) PDF, 255 KB...

  19. Immunology Research Plasmids and Resources

    Type
    Collection
    ...superfamily, member 2) DIF, TNF-alpha, TNFA, TNFSF2 TRAC T cell receptor alpha constant - TRAJ1 T cell receptor...receptor, beta - IL16 interleukin 16 (lymphocyte chemoattractant factor) FLJ16806, FLJ42735, FLJ44234, HsT19289...receptor superfamily, member 10c, decoy without an intracellular domain CD263, DCR1, LIT, MGC149501, MGC149502...

  20. Brain Initiative Collection

    Type
    Collection
    ...pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G...pAAV-TREtight-mTagBFP2-B19G helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA...
Showing: 21 - 40 of 506 results