We narrowed to 35 results for: U6

1. Plasmids 101: Expressing shRNAs for RNA Interference

   Type

   Blog Post

   ...transcribe small RNAs. The most common promoters are U6, H1, and 7SK. These are strong promoters with no ...

2. 28 Hot Plasmid Technologies from 2015

   Type

   Blog Post

   ...cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive (H1, U6, 7SK) or inducible shRNA-miR30...of the available sgRNA-cargo constructs utilize a U6 promoter and contain the RNA cargo cassette inserted...

3. Sequencing Primers

   Type

   Guide

   ...LKO.1 5' GACTATCATATGCTTACCGT (Weinberg Lab) Human U6 promoter, forward primer LucNrev CCTTATGCAGTTGCTCTCC...LKO.1 5' GACTATCATATGCTTACCGT (Weinberg Lab) Human U6 promoter, forward primer LNCX AGCTCGTTTAGTGAACCGTCAGATC... forward primer mU6-F CAGCACAAAAGGAAACTCACC Mouse U6 promoter, forward primer Myc GCATCAATGCAGAAGCTGATCTCA... TRC-F CAAGGCTGTTAGAGAGATAATTGGA (Root lab) Human U6 promoter, forward primer Ubx-F AACTCGTACTTTGAACAGGC...

4. Sequencing Primers

   Type

   Guide

   ...LKO.1 5' GACTATCATATGCTTACCGT (Weinberg Lab) Human U6 promoter, forward primer LucNrev CCTTATGCAGTTGCTCTCC...LKO.1 5' GACTATCATATGCTTACCGT (Weinberg Lab) Human U6 promoter, forward primer LNCX AGCTCGTTTAGTGAACCGTCAGATC... forward primer mU6-F CAGCACAAAAGGAAACTCACC Mouse U6 promoter, forward primer Myc GCATCAATGCAGAAGCTGATCTCA... TRC-F CAAGGCTGTTAGAGAGATAATTGGA (Root lab) Human U6 promoter, forward primer Ubx-F AACTCGTACTTTGAACAGGC...

5. Protocol - pLKO.1 – TRC Cloning Vector

   Type

   Protocol

   ...www.addgene.org/10878/ . Description Vector Element U6 Human U6 promoter drives RNA Polymerase III transcription...

6. CRISPR Plasmids - C. elegans

   Type

   Collection

   ...47549 pDD162 (Peft-3::Cas9 + Empty sgRNA) R07E5.16 U6 yes, cut Goldstein 42250 DR274 T7 BsaI In vitro transcription...

7. Promoters

   Type

   Guide

   ...Tetracycline response element promoter U6 Constitutive Human U6 nuclear promoter for small RNA expression...

8. Promoters

   Type

   Guide

   ...Tetracycline response element promoter U6 Constitutive Human U6 nuclear promoter for small RNA expression...

9. CRISPR References and Information

   Type

   Collection

   ...and BsmBI site for cloning in gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF, 49 KB Vosshall...

10. Retrovirus Plasmids

    Type

    Collection

    ...and Neo resistance Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses...

11. Kazuhiro Oka Lentiviral Vectors

    Type

    Collection

    ...shRNA against mouse TNF-alpha mRNA from the mouse U6 promoter pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro 73022 ...

12. Genomic Deletions in Mammalian Cell Lines

    Type

    Collection

    ...of the 20-mer is not G. sgRNA expression from the U6 promoter of the pX330 vector is enhanced by the inclusion... for each sample and sequence each colony using a U6 promoter forward primer: CGTAACTTGAAAGTATTTCGATTTCTTGGC...

13. 22 Hot Plasmid Technologies from 2014

    Type

    Blog Post

    ...ES) cells. These vectors, termed RUSH (For ROSA26 U6 short hairpin) and CRUSH (Conditional RUSH) use Cre-mediated...

14. Luciferase Plasmid Collection

    Type

    Collection

    ...Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla...

15. 27 Hot Plasmids from 2016

    Type

    Blog Post

    ...backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter. Addgene...