... base editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements...fusions, modifying the linker region between Cas variant and deaminase to adjust the editing window, or ...systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing window...deaminase (AID). Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...

...Arabidopsis, wheat, maize, rice, tomato, and a variety of other species. In addition to individual plasmids...tables below highlight plasmids spanning a wide variety of molecular biology technologies: from genome ...selection of plasmids expressing different functional variants of Cas9 and empty gRNA backbones. A few examples...Daniel Voytas. A CRISPR/Cas9 Toolkit for use in a variety of plant species (e.g. maize and Arabidopsis) and...

...molecule is not required to be fluorescent. A variety of organic dyes and fluorescent proteins have been... Mammalian Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian...Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange...LSSmOrange mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly used with Clover pGWF1 Cyan & Yellow ...

...miRNAs, and use a wide variety of drug selection markers. See article for more variations. Campeau 39481 pLenti-puro... See article for more cre-lox shRNA expression variants. Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion...fusion. See plasmids 36083 and 36086 for other FP variants. Tsoulfas 41393 pCW57.1 3rd Inducible lentiviral...

... short ~100 nt guide RNA ( gRNA ) that uses 20 variable nts at its 5’ end to base pair with a target genomic... and LbCpf1) for use in mammalian cells SpCas9 variants with no detectable off-target effects Modified...specifity of Staphylococcus aureus Cas9 (SaCas9 KKH variant) Engineered CRISPR-Cas9 nucleases with altered ...

...publications, focused on somatic variants found in lung adenocarcinoma as well as variants found across cancer types...Research Plasmids and Resources Addgene has a large variety of resources for use in cancer signaling studies...

...Monomer? Addgene's plasmid repository contains a variety of fluorescent protein plasmids. Use this guide...comprehensive collection consisting of ORFs tagged with a variety of fluorescent proteins, as well as over 100 empty...Community Resources FPbase from Talley Lambert at Harvard Medical School Fluorescent Biosensor Database designed...

...since then scientists have engineered numerous GFP-variants and non-GFP proteins that result in a diverse ...fluorescent proteins created by Talley Lambert at Harvard Medical School More References below Jump to: Blue...Thorn Lab Vectors - Includes tagging with a wide variety of colors, some specifically optimized for yeast...FPbase : A database created by Talley Lambert at Harvard Medical School Zeiss Microscopy : Optical Highlighters...

...Addgene Organizing Committee: Dr. Constance Cepko, Harvard Medical School Dr. Melina Fan, Dr. Susanna Bachle...Rego, Addgene Confirmed Speakers Constance Cepko Harvard Medical School Ali Cetin Allen Institute for Brain...e.g., publication's permission). Connie Cepko, Harvard Medical School Ali Cetin and Shenqin Yao, Allen...

... a large number of zinc fingers that recognize various nucleotide triplets. With some trial and error,...from each other by two amino acids, their repeat-variable di-residue (RVD). Ultimately, it is this RVD that...31 repeats can be assembled and inserted into a variety of backbones in just a few steps ( Figure 2 ). ...

...gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and your genomic...gRNA activity. Know your target. Which species or variant of Cas9 ( S. pyogenes, S. aureus etc.) was this...compatible with a single species or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must be used with...elegans TCCGCTACCATAGGCACCA 71479 cut VQR Cas9 variant 26680661 Fire dpy‐10 C. elegans GCTACCATAGGCACCACGAG...

... to find plasmids that can be used to engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses... immunogenicity. Different types of viruses also vary in the composition of their viral genome, with some...NIH provides basic guidelines for working with various types of viruses; however, these guidelines may...

...independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited to a ...denoted as such. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...plasmids for assembly of single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs...into one vector, and plasmids that provide Cas9 variants. All components are then assembled into a single...

...nervous system function. While there is wide variability in symptoms even within patients with the same...repository Tissue Banks (Link opens in a new window) Harvard Brain Bank (Link opens in a new window) Autism ...algorithms for data analysis to calculate heart rate variability (Link opens in a new window) Protocols.io - protocol...

...ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR and variants Jaws stGtACR iC++ OPN3 PdCO PPO Bidirectional...AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI 20071 pACAGW-ChR2-Venus-AAV...

...including calcium sensors, under the control of various promoters. Sensor Green Calcium Sensors GCaMP8 ...Calcium Sensor: GCaMP7c 105321 pGP-AAV-syn-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Constitutive 1 Kim ...Kim 105322 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Cre 1 Kim Calcium Sensor: GCaMP6f...

... your protein of interest resides. There are a variety of methods for determining subcellular localization...fluorescent fusion proteins that are targeted to various organelles or subcellular structures. You should...proteins. General Structures The cell contains a variety of organelles and subcellular structures each with...

...to generate and characterize single nucleotide variant lines in an isogenic cellular background. All cell...create cell lines with endogenously-tagged gene variants or to differentiate human iPSCs into other cell...

... activating or upregulating gene expression in various model organisms....specific locus. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...

...for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s ...for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2-dTom-nlsdTom...