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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 771 results for: Vars;

Showing: 21 - 40 of 771 results

  1. Plan Your Experiment

    Type
    Collection
    ... window from the PAM sequence, and base editor variants often have different optimal editing windows. ... and gRNA(s) in your target cells. There are a variety of ways to deliver and express these components...delivery . As mentioned above, CRISPR efficiency will vary based on the method of delivery and the cell type.... A mixed population will contain cells with a variety of modifications and will likely contain a subset...subset of wild type cells. If you are screening a variety of gRNAs or a new type of Cas enzyme, validating...

  2. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...molecule is not required to be fluorescent. A variety of organic dyes and fluorescent proteins have been... Mammalian Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian...Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange...LSSmOrange mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly used with Clover pGWF1 Cyan & Yellow ...

  3. Lentivirus Plasmids

    Type
    Collection
    ...miRNAs, and use a wide variety of drug selection markers. See article for more variations. Campeau 39481 pLenti-puro... See article for more cre-lox shRNA expression variants. Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion...fusion. See plasmids 36083 and 36086 for other FP variants. Tsoulfas 41393 pCW57.1 3rd Inducible lentiviral...

  4. Cancer Research Plasmids and Resources

    Type
    Collection
    ...publications, focused on somatic variants found in lung adenocarcinoma as well as variants found across cancer types...Research Plasmids and Resources Addgene has a large variety of resources for use in cancer signaling studies...

  5. Fluorescent Protein Guide

    Type
    Collection
    ...Monomer? Addgene's plasmid repository contains a variety of fluorescent protein plasmids. Use this guide...comprehensive collection consisting of ORFs tagged with a variety of fluorescent proteins, as well as over 100 empty...Community Resources FPbase from Talley Lambert at Harvard Medical School Fluorescent Biosensor Database designed...

  6. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...since then scientists have engineered numerous GFP-variants and non-GFP proteins that result in a diverse ...fluorescent proteins created by Talley Lambert at Harvard Medical School More References below Jump to: Blue...Thorn Lab Vectors - Includes tagging with a wide variety of colors, some specifically optimized for yeast...FPbase : A database created by Talley Lambert at Harvard Medical School Zeiss Microscopy : Optical Highlighters...

  7. TALEN Guide

    Type
    Collection
    ... a large number of zinc fingers that recognize various nucleotide triplets. With some trial and error,...from each other by two amino acids, their repeat-variable di-residue (RVD). Ultimately, it is this RVD that...31 repeats can be assembled and inserted into a variety of backbones in just a few steps ( Figure 2 ). ...

  8. Validated gRNA Sequences

    Type
    Collection
    ...gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and your genomic...gRNA activity. Know your target. Which species or variant of Cas9 ( S. pyogenes, S. aureus etc.) was this...compatible with a single species or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must be used with...elegans TCCGCTACCATAGGCACCA 71479 cut VQR Cas9 variant 26680661 Fire dpy‐10 C. elegans GCTACCATAGGCACCACGAG...

  9. Viral Vectors

    Type
    Collection
    ... to find plasmids that can be used to engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses... immunogenicity. Different types of viruses also vary in the composition of their viral genome, with some...NIH provides basic guidelines for working with various types of viruses; however, these guidelines may...

  10. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited to a ...denoted as such. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...plasmids for assembly of single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs...into one vector, and plasmids that provide Cas9 variants. All components are then assembled into a single...

  11. Western Blot

    Type
    Protocol
    ...science. General Guidelines Acrylamide percentage varies in SDS-PAGE gels. Use lower percentage acrylamide...buffer. Pro-Tips The volume of lysis buffer will vary depending on the size of your cell pellet but will...between 250–1000 µL . The ideal lysis buffer will vary depending on the cellular location of the protein...sample. Pro-Tip The ideal total protein loaded will vary between samples and target proteins but is typically... 1X TBST. Pro-Tip The ideal blocking buffer will vary between antibodies. Refer to the manufacturer's ...blocking buffer. Pro-Tip The optimal concentration will vary between antibodies but is typically between 1–10...

  12. Rett Syndrome

    Type
    Collection
    ...nervous system function. While there is wide variability in symptoms even within patients with the same...repository Tissue Banks (Link opens in a new window) Harvard Brain Bank (Link opens in a new window) Autism ...algorithms for data analysis to calculate heart rate variability (Link opens in a new window) Protocols.io - protocol...

  13. Optogenetics AAV Preps

    Type
    Collection
    ...ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR and variants Jaws WiChR stGtACR iC++ OPN3 PdCO PPO...AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI 20071 pACAGW-ChR2-Venus-AAV...

  14. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...increases reproducibility, as it eliminates the variation associated with repeated transient transfection...lots can promote or inhibit transfection. Test a variety of brands and FBS lot numbers to find one suitable...transduction efficiency. Infection efficiency will vary between cell lines. It is not recommended that lentiviral...that already contain 0.5 mL of virus solutions at various dilution. Make sure to use the polybrene-containing...transgene has integrated in different locations in the various cells in the culture. This is because lentiviral...of the lentivirus used, cells may also exhibit varying numbers of integration events. In other words, ... transgene in their genome. This can result in varying expression levels of the transgene from different...

  15. Luciferase Plasmid Collection

    Type
    Collection
    ...enzyme responsible for bioluminescence found in a variety of organisms, ranging from bacteria to aquatic ...the protein is available, making it ideal for a variety of applications. To learn more and plan your experiment...Highlighted Tools Modified/Novel Luciferases Engineered variants of luciferases with altered emission, substrate...

  16. Bacterial Expression Systems

    Type
    Collection
    ...whose expression levels can be controlled by a variety of small molecules, light, and temperature, in ...#1000000037) contains specific bacterial promoters that vary in strength and that can be used to reliably drive...constructs. The Zinc Finger Consortium page contains a variety of reagents for engineering and expressing zinc...

  17. Tetracycline Inducible Expression

    Type
    Collection
    ...expression, the system has been engineered with a variety of improvements. A synthetic Tet Response Element...). rtTA3 and Tet-On 3G : further improved rtTA variants with lower background activity and greater sensitivity...Lentiviral Tet-On inducible RNAi with miR-E (miR-30 variant)-based backbone rtTA-Advanced TRE3G Johannes Zuber...

  18. Biosensor AAV Preps

    Type
    Collection
    ...including calcium sensors, under the control of various promoters. Sensor Green Calcium Sensors GCaMP8 ...Calcium Sensor: GCaMP7c 105321 pGP-AAV-syn-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Constitutive 1 Kim ...Kim 105322 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Cre 1 Kim Calcium Sensor: GCaMP6f...

  19. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... your protein of interest resides. There are a variety of methods for determining subcellular localization...fluorescent fusion proteins that are targeted to various organelles or subcellular structures. You should...proteins. General Structures The cell contains a variety of organelles and subcellular structures each with...
Showing: 21 - 40 of 771 results