..., Cas13a does not exhibit the collateral RNA degradation seen in bacteria. ID Plasmid Gene/Insert Promoter...rather than DNA. Fusing the catalytic domain of ADAR2(E488Q) adenosine deaminase to catalytically dead...

...distinguishable thanks to the use of phenol red. Image adapted from Zolotukhin, S., et al. "Recombinant adeno-...indicates the location of the purified AAV. Left image adapted from Zolotukhin, S., et al. "Recombinant adeno-... the purified AAV using option #1. Right image adapted by permission from Macmillan Publishers Ltd: Nature...proteins VP1, VP2, VP3; M protein marker. Image adapted from Strobel Benjamin, Miller Felix D., Rist Wolfgang...

...Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 106292 pLS-SV40-mP-Rluc Renilla Insertion...Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 108228 pGreen2x35SLUC Firefly/Renilla Creating...

... and stereotaxic (STX) delivery in adult mice. Adapted from Allen Institute's Genetic Tools Atlas (Link...Ding Y, Somasundaram S, Miller JA, Kalmbach BE, Radaelli C, Gore BB, Weed N, Omstead V, Bishaw Y, Shapovalova...

..., Cas13a does not exhibit the collateral RNA degradation seen in bacteria. Want more information on the...

...SynBio Depositing Labs Hal Alper J. Chris Anderson Adam Arkin Gabor Balazsi Matthew Bennett Carolyn Bertozzi...

...protection added to 3’ tail of pegRNA to prevent RNA degradation For more in-depth information on these PE tools...

...described in (Gassaway et al., Nature Methods 2022) is adapted from the synthetic human phosphoproteome. The iSPI...

...method. Validated in multiple organisms. Dan Voytas Adam Bogdanove Joung Lab TAL Effector Engineering Reagents...

...Tools CRISPR Blog Posts The CRISPR system has been adapted to allow tagging of proteins expressed from their...

...TGTCCTCATCCCCGTCCCgccgcggcgccaccgtCAGCTTCCACAACATCAA adam15 TAL3016 & TAL3017 TGTTTCTGGCGGACTCTCgtcaccattcttctgtTTGAAGGCACACCTTTGA...

...window) Marcinkiewcz CA, Mazzone CM, D'Agostino G, Halladay LR, Hardaway JA, DiBerto JF, Navarro M, Burnham...

...CLOVER Center (Link opens in a new window) and was adapted from Challis et al. (2022). Text version of the...

...transfection of CRISPR/Cas9 plasmids may be successfully adapted to numerous cell types using preferred culture ...

...cfSGFP2-N Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3 Mitochondria...

...selection with carbenicillin; however it can be adapted for any antibiotic through the use of a selection...

...protocol was developed for 293T cells but can be adapted to a variety of target cell lines and selection...

...HEK293T cell line should work. This approach can be adapted for different cell lines and different transfection...

...protocol was developed for A549 cells but can be adapted to a variety of target cell lines and selection...

...Escherichia coli TadA, a tRNA adenosine deaminase. Like cytosine base editors, the evolved TadA domain is fused...on a second ADAR variant that displays improved specificity, and editors carrying an ADAR truncation that...break, Cas3 initiates single-strand DNA degradation. This degradation can continue for many kilobases in one...Publications CRISPR Overview Bacteria have an interesting adaptive immune response that detects and eliminates foreign...target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most popular genome...inhibiting Cas9’s endonuclease domain; or inducing degradation of the Cas9 protein. Most Acr proteins are specific... or dCas9 to create the adenine base editor. The TadA domain has also been further evolved to catalyze...