We narrowed to 344 results for: ada
-
TypeCollection..., Cas13a does not exhibit the collateral RNA degradation seen in bacteria. ID Plasmid Gene/Insert Promoter...rather than DNA. Fusing the catalytic domain of ADAR2(E488Q) adenosine deaminase to catalytically dead...
-
Luciferase Plasmid Collection
TypeCollection...Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 212935 pGL4.84(hRlucCP/Puro) RapidResponse...Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 108228 pGreen2x35SLUC Firefly, Renilla Creating... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection... and stereotaxic (STX) delivery in adult mice. Adapted from Allen Institute's Genetic Tools Atlas (Link...Somasundaram, S., Miller, J. A., Kalmbach, B. E., Radaelli, C., Gore, B. B., Weed, N., Omstead, V., Bishaw... -
Tetracycline Inducible Expression
TypeCollection...stimuli like hormones or metal ions, scientists have adapted an antibiotic resistance operon from E. coli : ...Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP... -
CRISPR Plasmids - RNA Targeting
TypeCollection..., Cas13a does not exhibit the collateral RNA degradation seen in bacteria. Want more information on the... -
Synthetic Biology - Overview
TypeCollection...SynBio Depositing Labs Hal Alper J. Chris Anderson Adam Arkin Gabor Balazsi Matthew Bennett Carolyn Bertozzi... -
CRISPR Plasmids - Prime Edit
TypeCollection...protection added to 3’ tail of pegRNA to prevent RNA degradation For more in-depth information on these PE tools... -
Promega Plasmid Collection
TypeCollection...investigating RNA regulation, tracking protein degradation, analyzing CRISPR-mediated gene editing outcomes... -
Protocol - Over-Agar Antibiotic Plating
TypeProtocol...selection with carbenicillin; however it can be adapted for any antibiotic through the use of a selection... -
TALEN Plasmids and Kits
TypeCollection...method. Validated in multiple organisms. Dan Voytas Adam Bogdanove Joung Lab TAL Effector Engineering Reagents... -
CRISPR Plasmids - Tagging
TypeCollection...Tools CRISPR Blog Posts The CRISPR system has been adapted to allow tagging of proteins expressed from their... -
Rinehart Lab Phosphoprotein Reagents
TypeCollection...resource described in Galloway et al. (2022) is adapted from the synthetic human phosphoproteome. It subdivides... -
Fluorescence Titering Assay
TypeProtocol...protocol was developed for 293T cells but can be adapted to a variety of target cell lines and selection... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection...sites. Created with BioRender.com. Scientists have adapted these systems to activate/inactivate genes when... -
CRISPR References and Information
TypeCollection...pooled library screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR... -
TALENs for Endogenous Zebrafish Genes
TypeCollection...TGTCCTCATCCCCGTCCCgccgcggcgccaccgtCAGCTTCCACAACATCAA adam15 TAL3016 & TAL3017 TGTTTCTGGCGGACTCTCgtcaccattcttctgtTTGAAGGCACACCTTTGA... -
General Transfection
TypeProtocol...HEK293T cell line should work. This approach can be adapted for different cell lines and different transfection... -
Colony Formation Titering Assay
TypeProtocol...protocol was developed for A549 cells but can be adapted to a variety of target cell lines and selection... -
Deisseroth INTRSECT Collection
TypeCollection...window) Marcinkiewcz CA, Mazzone CM, D'Agostino G, Halladay LR, Hardaway JA, DiBerto JF, Navarro M, Burnham... -
Caltech Systemic Capsids
TypeCollection...CLOVER Center (Link opens in a new window) and was adapted from Challis et al. (2022). Text version of the...