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We narrowed to 36 results for: broad host range

Showing: 21 - 36 of 36 results

  1. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...epithelium (RPE). AAVs, on the other hand, have broad tropism in the eye. AAV-2, the first identified ...engineered AAV serotypes that further expands the range of AAV properties is available. While only natural...needed. Additionally, AAVs do not integrate in the host genome but still lead to long-term (>10 years) transgene...

  2. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ... those that are responsible for integration into host chromosomes, further minimizing potential activation...AAV vectors are also capable of infecting a wide range of cell types including but not limited to muscle...specific promoters may also help AAV vectors evade host immunity. While basic research and other ongoing...clinical trials support the utility of AAV as a broadly applicable gene delivery tool, aspects of AAV biology...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...invading phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas)...vectors that can be combined with Cas9 in a wide range of applications. Full references are below. The ... studying the function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo...

  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...new horizons to modify genomes in a broad range of bacterial hosts. This fantastic tool is now available... relatives of E. coli so it can be used in a broad range of strains. Thus, pORTMAGE simultaneously allows...repair (MMR) system needs to be repressed in the host strain. To address this problem, Csaba Pál’s lab...on the yeast, Saccharomyces cerevisiae, as a key host for the production of their products, including ...AID-tagged proteins after the introduction of auxin range from 10-20 minutes, making AID an appealing tool...series also allows for protein expression under a range of auxotrophic markers. Read more about the Ralser...

  5. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ...optimized” so that it more closely resembles that of the host organism, it may express protein more efficiently... synthesis companies often work within a narrow range for GC content. Rare codons also are not good predictors...Proteobacteria to mammalian expression vectors. And more broadly, their data revealed that the longer a natural ...

  6. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...Cas9 and Adeno EA. Or if you're looking for a broader range of CRISPRs plasmid tools, find more plasmids...36 kb in size, which does not integrate into the host genome, removing the risk of oncogenicity or genotoxicity...disruption frequencies in the various cell types ranged from 18% to 65% (Maggio et al., 2014). An alternative...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature 516:423...

  7. Hot Plasmids Spring 2024

    Type
    Blog Post
    ... or reverse transcriptase enzymes, inhibition of host cell proteins, and modifications to the pegRNA. ...recently used a CRISPRi screen to identify additional host factors impacting prime editing and found the protein...functionally reconstituting coding sequences for a range of applications, including CRISPR activation by ...simple yet valuable tool for microglia research and broader therapeutic applications.  Find microglia-targeting...

  8. CRISPR Guide

    Type
    Collection
    ... DNA, which scientists have modified for a wide range of genome engineering techniques . Collectively,... often found in plants, expanding the targeting range for plant genome engineering. Read more about Cas9...non-specifically. Cas12a also has an expanded targeting range to AT-rich regions, as it recognizes TTTV (V = A.... R., Maxwell, K. L., & Doudna, J. A. (2017). A Broad-Spectrum inhibitor of CRISPR-Cas9. Cell , 170 (6...& Liu, D. R. (2018). Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature...screens in E. coli identify essential genes and phage host factors. PLOS Genetics . 14 (11), e1007749. PMID...with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nature Biotechnology...

  9. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...considered to not integrate into the host genome. When wild-type AAVs enter a host cell, they uncoat in the nucleus...sites of the host genome can occur, although at a low frequency which has been estimated to range from 0.01%...serotype, and a very high infectivity across a broad range of cell types in vivo. AAV-DJ has been optimized...adenovirus or herpes simplex virus to replicate in the host cell and complete the lytic cycle. AAVs are therefore...establish latent infections by integrating into the host genome at a specific site, the AAVS1 site on human...proteins and are therefore unable to integrate in the host’s genome. Instead, the linear rAAV DNA is typically...avoids the effects local chromatin structures in the host genome can have on transgene expression and the ...

  10. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...to infected host cells. When these antibiotics are added to the growth medium of the host cells, they ...by their ability to integrate their genomes into hosts in order to continue their lifecycle and replicate...models, such as stable long-term expression in a host and low immunogenicity. This guide contains many...into DNA before being integrated in the genome of a host. Going from RNA to DNA is the reverse of what you...Gamma-Retroviral Vectors The genome of gamma-retroviruses ranges from 9–11 kb, encoded on RNA (Figure 1). This RNA...provirus, the cDNA that will be integrated into a host genome. This single strand of RNA contains packaging... anything in between will be integrated into the host genome. To produce gamma-retroviral vectors, these...

  11. Lentiviral Vector Guide

    Type
    Guide
    ...to infected host cells. When these antibiotics are added to the growth medium of the host cells, they ...by their ability to integrate their genomes into hosts in order to continue their lifecycle and replicate...non-dividing cells, enable stable long-term expression in a host, and exhibit low immunogenicity. This guide contains...into DNA before being integrated in the genome of a host. Going from RNA to DNA is the reverse of what you...Lentiviral Vectors The genome of lentiviruses typically ranges from 8–10 kb, encoded on RNA (Figure 1). This RNA...provirus, the cDNA that will be integrated into a host genome. This single strand of RNA contains packaging... anything in between will be integrated into the host genome. To produce lentiviral vectors in the laboratory...

  12. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...particularly retroviral vectors because they target a broader range of cells and their integration into the genome...immunogenic proteins generated Stable integration into host genome and stable expression of the transgene ...protein (VSV-G) that improves the stability and broadens the cellular tropism of the viral particles produced...determine their concentration. Three methods are broadly used for virus titration: the p24 ELISA dosing ...

  13. Finding Your Science Policy Path

    Type
    Blog Post
    ...variety of backgrounds with technical expertise that ranges from astronomy to bioethics to chemical engineering...federal or state level some professional societies host “Hill Days” where they train and bring researchers...can inform your move into science policy. Read broadly about the historical roots of science policy to...

  14. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...large cargo capacity, minimal cytotoxicity, and broad host-tropism. These tools open up many powerful avenues...differ from each other by their light/dark affinity range (130nM to 4.7µM for iLID nano and 800nM to 47µM ...iLID micro; Figure 1). With this great affinity range, these LIDs have been shown to be useful tools for...quantified. This strategy relies on the current range of mitochondrially targeted photoactivatable and...reduced system leakiness and increased the dynamic range, resulting in more user-friendly and tunable tools...et al 2015[2]). The proteins encoded in this set range in size from 4 to 156 kDa, enabling the user to ...variant of PSmOrange, a photoswitchable fluorescent protein which transitions from emitting orange (λ= 565nm...

  15. Molecular Biology Reference

    Type
    Guide
    ...conjugation), usually providing a benefit to the host, such as antibiotic resistance. This benefit can...plasmid exists in a symbiotic relationship with the host cell. Like the bacterial chromosomal DNA, plasmid... providing a survival advantage to the bacterial host. Each bacterium can contain multiple copies of an... our popular Plasmids 101 series covers topics ranging from the newest breakthroughs in plasmid technologies...by scientists today come in many sizes and vary broadly in their functionality. In their simplest form,...

  16. CRISPR Guide

    Type
    Guide
    ... DNA, which scientists have modified for a wide range of genome engineering techniques . Collectively,... often found in plants, expanding the targeting range for plant genome engineering. Read more about Cas9...non-specifically. Cas12a also has an expanded targeting range to AT-rich regions, as it recognizes TTTV (V = A.... R., Maxwell, K. L., & Doudna, J. A. (2017). A Broad-Spectrum inhibitor of CRISPR-Cas9. Cell , 170 (6...& Liu, D. R. (2018). Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature...screens in E. coli identify essential genes and phage host factors. PLOS Genetics . 14 (11), e1007749. PMID...with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nature Biotechnology...
Showing: 21 - 36 of 36 results