We narrowed to 48 results for: ef1a
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TypeCollection...pAAV-Ef1a-sRGECO Ef1a sRGECO none Constitutive 8 Deisseroth 137126 pAAV-Ef1a-Con/Fon-sRGECO Ef1a sRGECO...pAAV-Ef1a-Con/Fon-GCaMP6F EF1a GCaMP6f none Cre and Flp dependent 5, 8 Deisseroth 137123 pAAV-Ef1a-Con/...-GCaMP6F EF1a GCaMP6f none Cre dependent 8 Deisseroth 137124 pAAV-Ef1a-Coff/Fon-GCaMP6F EF1a GCaMP6f none... pAAV-EF1a-Con/Fon-GCaMP6M EF1a GCaMP6m none Cre and Flp dependent 8 Deisseroth 137120 pAAV-Ef1a-Con/Foff...-GCaMP6M EF1a GCaMP6m none Cre dependent 8 Deisseroth 137121 pAAV-Ef1a-Coff/Fon-GCaMP6M EF1a GCaMP6m none... AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato EF1a GCaMP6s dTomato Cre dependent 1 Ting 105714 pAAV-Ef1a-fDIO-...pAAV-Ef1a-fDIO-GCaMP6s EF1a GCaMP6s none Flp dependent 8 Larsen 105715 pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s EF1a GCaMP6s...
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Optogenetics AAV Preps
TypeCollection...26968 pAAV-Ef1a-DIO ChETA-EYFP EF1a ChETA EYFP Cre dependent 1, 5, 9 Deisseroth 35497 pAAV-Ef1a-DIO C1V1...floxed-eNpHR-EYFP-WPRE-pA EF1a eNpHR EYFP Cre dependent 9 Deisseroth 26966 AAV-Ef1a-DIO eNpHR 3.0-EYFP EF1a eNpHR 3.0 ... Deisseroth 20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA EF1a ChR2/H134R mCherry Cre ...rg* Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a ChR2/H134R EYFP Cre dependent...mCherry Flp dependent 1 Cepko 55639 pAAV-Ef1a-fDIO hChR2(H134R)-EYFP EF1a ChR2/H134R EYFP Flp dependent rg* ...Constitutive 9 Deisseroth 35509 pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP EF1a ChR2(E123T/T159C) EYFP Cre dependent... PHPeB Dimidschstein 100061 pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry EF1a C1V1 (t/t) mCherry Cre dependent ... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...where common eukaryotic promoters (such as CMV or EF1A promoters found in many CRISPR plasmids) are not... -
Penn Vector Core Partnership with Addgene
TypeCollection...AAV5 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl Deisseroth AV-5-26968P 26968-AAV5 pAAV-Ef1a-DIO ChETA-EYFP... pAAV-Ef1a-DIO C1V1 (t/t)-TS-EYFP Optogenetics Karl Deisseroth AV-9-35498M 100061-AAV9 pAAV-Ef1a-DIO C1V1...AAV9 pAAV-Ef1a-DIO hChR2(E123A)-EYFP Optogenetics Karl Deisseroth AV-9-35509 35509-AAV9 pAAV-Ef1a-DIO hChR2...105547-AAV9 pENN.AAV.EF1a.eGFP.WPRE.rBG Control James M. Wilson AV-9-PV2453 45185-AAV9 AAV-EF1a-BbTagBY Control...Biosensor James M. Wilson AV-1-27056 27056-AAV1 pAAV-Ef1a-DIO EYFP Control Karl Deisseroth AV-1-ALL854 51502...Control James M. Wilson AV-2-27056 27056-AAV2 pAAV-Ef1a-DIO EYFP Control Karl Deisseroth AV-2-ALL854 51502...Control James M. Wilson AV-5-27056 27056-AAV5 pAAV-Ef1a-DIO EYFP Control Karl Deisseroth AV-5-PV0101 105530... -
Caltech Systemic Capsids
TypeCollection...140135 pAAV-EF1a-iCreV EF1a light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible...CamKIIa mCherry Control Deisseroth 114470 pAAV-Ef1a-mCherry EF1a mCherry Control Deisseroth 117382 hSyn1-eYFP...ChR2 Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a Activator, Cre-dependent...light-inducible recombinase iDreV Zeng 140137 pAAV-EF1a-iFlpV EF1a light-inducible recombinase iFlpV Zeng 164450...pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson 105547 pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control Wilson 114469 pAAV-CaMKIIa-mCherry... -
Brain Initiative Collection
TypeCollection...under the mammalian promoter (EF1a) Francois St-Pierre 179460-AAV1 pAAV-EF1a-DIO-JEDI-2P-WPRE Double floxed...eukaryotic Ef1a promoter after Cre-dependent recombination. Ofer Yizhar 198516-AAV5 pAAV_EF1a-DIO-PdCO-...signaling cascades. Michael Bruchas 139505-AAV8 pAAV-Ef1a-DIO-PPO-Venus AAV vector for Cre-dependent expression...expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory...signaling cascades. Michael Bruchas 139505-AAV9 pAAV-Ef1a-DIO-PPO-Venus AAV vector for Cre-dependent expression...signaling cascades. Michael Bruchas 140135-AAV1 pAAV-EF1a-iCreV can be used to generate AAV virus that will... -
AAV Molecular Tools
TypeCollection...System Activity Serotype PI 98747 pAAV-FLEX-EGFPL10a EF1a-driven and Cre-dependent EGFP-tagged ribosomal L10a...Luo 60658 pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato EF1a-driven, Flp-dependent Flp-dependent expression of...ablation. 2 Jessell , Azim 45580 pAAV-flex-taCasp3-TEVp EF1a-driven, Cre-dependent Cre-dependent, bicistronic... -
Cre-lox system
TypeCollection...pAAV-Ef1a-mCherry-IRES-Cre Cre and mCherry coexpression EF-1 alpha AAV Deisseroth 55635 pAAV-EF1a-sCre...AAV Deisseroth 55636 pAAV-EF1a-Cre Cre EF-1 alpha AAV Deisseroth 55638 pAAV-EF1a-vCre VCre EF-1 alpha AAV...similar plasmids pSicoR PGK puro , pSicoR-Ef1a-mCh-Puro , pSicoR-Ef1a-mCh and pSicoR-mCh-empty Lentiviral Jacks...hypoxia-responsive GFP reporter HRE Lentiviral Jacks 69570 pAAV-EF1a-N-CretrcintG Cre recombinase dependent on GFP (CRE-DOG...(CRE-DOG) EF-1 alpha AAV Cepko 69571 pAAV-EF1a-C-CreintG Cre recombinase dependent on GFP (CRE-DOG) EF...-specific expression ANF AAV Wehrens 121675 pAAV-EF1a-fDIO-Cre Cre EF-1 alpha AAV Engel 122518 pAAV-Syn-iCre...pTFUbiCre Cre Maize Ubiquitin Plant Birchler 140131 pAAV-EF1a-NCreV N-terminal Cre component split with Vivid ... -
Plasmids 101: Repressible Promoters
TypeBlog Post.... Many commonly-used promoters, such as T7, CMV, EF1A, and SV40, are always active and thus referred to... -
Neurodegeneration Plasmid Collection
TypeCollection...pLEX-EF1a ANXA11-mEmerald ANXA11 mEmerald EF1a ALS Jennifer Lippincott-Schwartz 164211 pLEX-EF1a ANXA11...Lindberg 166952 pLV-EF1a-KIF5A-HA-IRES-Puro KIF5A HA eF1a ALS Rosalind Segal 166953 pLV-EF1a-KIF5AR280H-HA-...170443 pCDH-EF1a-hDNAJC6-T2A-copGFP DNAJC6 copGFP eF1a Parkinson's Sang-Hun Lee 170445 pCDH-EF1a-hSNCA SNCA... 213554 pLv-EF1a-Parkin(K161N)-A92mKO2 PRKN mKO2 EF1a Parkinson's Lani Wu 213555 pLv-EF1a-Parkin(N273K...A92mKO2 PRKN mKO2 EF1a Parkinson's Lani Wu 213556 pLv-EF1a-Parkin(R104A)-A92mKO2 PRKN mKO2 EF1a Parkinson's ...Wu 213557 pLv-EF1a-Parkin(S65A)-A92mKO2 PRKN mKO2 EF1a Parkinson's Lani Wu 213558 pLv-EF1a-Parkin(W403A...A92mKO2 PRKN mKO2 EF1a Parkinson's Lani Wu 213559 pLv-EF1a-Parkin-A92mKO2 PRKN mKO2 EF1a Parkinson's Lani... -
Viral Production
TypeCollection...: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral...Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...Representative Empty Backbones Mammalian CMV, SV40, EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids...backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker pLenti CMV/TO GFP-Zeo DEST...Resources collection page Zebrafish CMV, h2afv, XlEef1a1 See our dedicated Zebrafish Plasmids and Resources... -
Lentiviral Prep Service
TypeCollection...encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang Pooled... -
Luciferase Plasmid Collection
TypeCollection...mammalian cell lysate Marcel Bruchez 108542 pLenti-EF1a-Luciferase-IRES-Blast-WPRE Firefly EF1α Lentiviral...moniter viral transfection Sidi Chen 118412 ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293 Firefly EF1α AAV expression... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...promoter, while Brainbow AAV is under control of the EF1a promoter. Livet et al., Nature. 2007. Nov 1;450... -
Tetracycline Inducible Expression
TypeCollection...controls GFP expression tTA Off Verma 26803 pEnt L1L3 EF1a-tTA-2 Contains Gateway L1L3 sites and EF1α promoter... -
Zhang Lab CRISPR Page
TypeCollection...backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for ... -
Promoters
TypeGuide...Strong mammalian promoter from human cytomegalovirus EF1a Constituitve Strong mammalian promoter from human... -
Chemogenetics Guide
TypeGuide...Neurons GFAP Glia CD68 Microglia Dlx Interneurons EF1a, CAG General expression Targeted expression. Depending... -
Genetic Code Expansion
TypeCollection...E. coli Bacterial TAG Abhishek Chatterjee 220800 EF1a-L274GMmMetRS-T2A-mCherry MetRS M. musculus azidonorleucine...