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We narrowed to 376 results for: epor

Showing: 21 - 40 of 376 results
  1. Lentivirus Plasmids

    Type
    Collection
    ...drives expression of RFP as a reporter. See plasmid 17618 for GFP reporter. Linzhao Cheng 17452 pLenti ...Expresses shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Luk...Expresses shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Stephan...86849 pBOB-EF1-FastFUCCI-Puro 3rd Encodes FUCCI reporters for cell cycle analysis. Puromycin selection. ...
  2. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific recombinase...recombinases , as well as useful empty backbones or reporters . Experimental Considerations Recombinase Recognition... plasmids. Return to top Recombinase-Dependent Reporter Plasmids Incorporating a fluorescent protein into...visualize which cells have undergone recombination. Reporter plasmids can also be useful for lineage tracing...
  3. Plasmids for Stem Cell Research

    Type
    Collection
    ...induced reprogramming to pluripotency. Stem Cell Reports. 2015 Apr 14;4(4):727-43. Woltjen Plasmid Mouse...self-regulating and nonintegrating viral vectors. Stem Cell Reports. 2014 Nov 6. pii: S2213-6711(14)00307-5. Jaenisch...Cells and B Lymphocytes to Neural Stem Cells. Cell Reports. 2014 Dec 11;9(5):1673-80. Parmar Fibroblasts Astrocytes...astrocytes by defined transcription factors. Stem Cell Reports. 2015 Jan 13;4(1):25-36. Broccoli Fibroblasts Sensory...
  4. CRISPR Guide

    Type
    Collection
    ...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...mapping multiprotein complexes in human cells. Cell Reports . 13 (3), 621–633. PMID: 26456817 Doench, J. G....
  5. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...whole range of expression levels for these two reporters simultaneously. By fusing sfGFP and/or mCherry...cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in...phage attachment sites. The levels of the two reporters, mCherry and sfgfp in this case, can be controlled...
  6. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several ...
  7. Biosensor AAV Preps

    Type
    Collection
    ...control) none Constitutive 1, 9 Yulong Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-...Archon1 EGFP Cre dependent 8 Edward Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST Syn ...ST none Cre dependent 1 Joseph Raimondo Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE ...none Constitutive 9 Francois St-Pierre Voltage Reporter: ASAP 225707 pAAV-hsyn-ASAP5-Kv2.1-WPRE Syn ASAP5...
  8. Zebrafish Plasmid Collection

    Type
    Collection
    ...editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large...studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study...using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein...
  9. Fluorescent Proteins: FRET

    Type
    Collection
    ...single-nanometer scale, scientists can use FRET to report on protein binding or conformational changes. Organic...our Biosensors Collection to find fluorescent reporters for metal ions, small molecules, and more! Popular...your experiment, be sure to refer to published reports and include appropriate controls to help interpret...
  10. AAV Viral Preps

    Type
    Collection
    ...on each item's material page. Actual titers are reported with each shipment. If you can't find the prep...Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti Monster...
  11. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...Endogenous Zebrafish Gene Targets Recent published reports have shown that engineered zinc finger nucleases...greater in a well-characterized bacterial two-hybrid reporter system but have NOT yet been tested for activity...
  12. Genetic Code Expansion

    Type
    Collection
    ... are true. Always express first with a control reporter gene: GFP for E. coli or mCherry-GFP for mammalian...RF1 function removed, with Ubiquitin-UAG-sfGFP reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP All...removed, ClpS inactivated, with Ubiquitin-UAG-sfGFP reporter George Church 174513 Syn61 No TCG, TCA, or TAG...
  13. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...to generate adenovirus expressing a variety of reporters as well as the Cre and Flp recombinases. Genes...
  14. AAV Molecular Tools

    Type
    Collection
    ...glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells. 5, 8 Don...express Cre, Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells. 5, 8 Don...
  15. Viral Production

    Type
    Collection
    ...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...recipient's initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...
  16. COVID-19 Resources

    Type
    Collection
    ... also lists several luciferase and fluorescent reporter plasmids that have been used for measuring viral...acids SARS-CoV-2 main protease (Mpro) activity reporters Reverse transcriptase, protease, and polymerase...
  17. TALEN Plasmids and Kits

    Type
    Collection
    ... pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based validation assay...N- and C-terminal domains (N153AA, C47AA). The reporter vector pGL4-SSA can be used for single-strand ...
  18. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains...their reporter mRNAs. Using the TRICK plasmids, Halstead et al demonstrated that 91% of reporter mRNAs...customized fluorescent reporters can be challenging enough, but incorporating multiple reporters into a single ...pyralis) reporter plasmids for use in high-throughput screening of pre-mRNA splicing. This reporter system...widely used luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids have been...protein function, thereby creating a single-molecule reporter of intracellular processes. Multimerization via...the cognate att sites are supplied as individual reporter constructs or as the fully assembled pMemoryArray...
  19. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ... the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains...proteins to allow translation to occur. For a TRICK reporter mRNA this displacement leads to a change in the...GFP protein that binds to the coding region of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) an RFP...translation, both GFP and RFP are bound to the reporter, causing it to appear yellow. The act of translation...of the mRNA, leaving only a red FP bound to the reporter. Because it contains a nuclear localization signal...copies of the FP-coat protein fusions to their reporter mRNAs. Using translation inhibitors cycloheximide...is necessary for removal of GFP from the TRICK reporter. Delayed translation is known to be important ...
  20. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...the promoter of the reporter gene to activate expression (Figure 2c). The reporter gene then allows detection...genes as reporters and some that repress instead of activate transcription. The HIS3 reporter system (...sequence (UAS) of its target gene, in this case a reporter gene (e.g. luciferase or GFP), regardless of whether...co-expressed in a yeast strain that also contains a reporter gene cloned downstream of a promoter containing...referred to as the “bait”, Figure 2b) cannot activate reporter gene expression on its own, but, if the two POI...Gal4 activator, bacterial LexA DBD and the lacZ reporter gene (3, 4), there are now many variations, including...to have Y2H systems that avoid transcriptional reporters altogether; In the split-luciferase system, intein-mediated...
Showing: 21 - 40 of 376 results