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  1. AAV Production in HEK293 Cells

    Type
    Protocol
    ... until fully dissolved. Pro-Tip This step is challenging due to the high viscosity of PEG. Stirring under...

  2. CRISPR Library Amplification

    Type
    Protocol
    ...Quality Control (QC) Pooled libraries can be challenging and expensive to ensure adequate quality, but...

  3. CRISPR Plasmids - Tagging

    Type
    Collection
    ... your gene of interest Mendenhall and Myers Tagging System The Eric Mendenhall and Richard Myers labs ...homology arm cloning: Mendenhall & Myers FETCh-seq Protocol 134.7 KB Mendenhall and Myers Tagging Plasmids... the homology arms for the donor plasmid. The Mendenhall and Myers labs have also provided their protocol...

  4. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ... Method Full Target Site (5' to 3') Left half-site Right half-site CACNA1F OZ507 and OZ508 OPEN OPEN gCGTGACCTCgctgatAAGAAGAGg...on the length of the spacer sequence between the half-sites (see Nuclease Expression Vectors for more ...and OZ540 OPEN OPEN tTGCATCCACggcagTCGGCAGTGc Encephalopsin (Opn3) OZ541 and OZ542 OPEN OPEN aTCCATCCCAaacacatGTGGCAGAAt...

  5. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Podgorski Calcium HaloCaMP1 chemigenetic calcium indicator for fluorescence using HaloTag ligands Bright ...Schreiter Calcium WHaloCaMP1 chemigenetic calcium indicator for fluorescence using HaloTag ligands A modular...30875-83. Wolf Frommer Trehalose Single fluorescent protein biosensor for trehalose Rapid construction of...Proc Natl Acad Sci U S A. 2019 Aug 1. Alexander Gottschalk Voltage Near-infrared fluorescent voltage indicator...

  6. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...were not plated, freeze half of the cells for future plating. Plate the other half for screening and primer...using the Qiagen Miniprep Kit. Zhang S, Cahalan MD. J. Vis. Exp . 2007. PubMed . Transformation... using the heat shock method. Froger A, Hall JE. J. Vis. Exp . 2007. PubMed . Molecular cloning...

  7. Zhang Lab CRISPR Page

    Type
    Collection
    ..., Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, Marraffini LA, Bao G, Zhang F. Nat... CRISPR-Cas9 knockout screening in human cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelsen...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods . 2014 Aug;11(8):783-4....WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang...

  8. CRISPR Guide

    Type
    Collection
    ...less widely used in bacteria due to technical challenges, bacterial CRISPR libraries have been developed... S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., Cradick, T. J., Marraffini, L. A., Bao, G....., Gootenberg, J. S., Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., Koonin, E. V., Sharp...790. PMID: 38216671 Klompe, S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019). Transposon-encoded...Communications . 9 (11), e1007749. PMID: 30575746 Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott...cells. Science . 343 (6166), 84–87. PMID: 24336571 Shalem, O., Sanjana, N. E., & Zhang, F. (2015). High-throughput...Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall, E. M., & Myers, R. M. (2015). CETCh-seq: CRISPR...

  9. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase and Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters...experiments possible, but its short luminescence half-life makes it too dim for many experimental systems...wavelengths : NanoLuc® with either SNAP‐tag or HaloTag7 tag in which labeling of the self‐labeling tag...biosensor that utilizes Renilla luciferase and a Halo tag to assay mRNA decay in real time. pKC-4.04, ...

  10. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...AiE0027m_3xC3 SYFP2 Ventromedial hypothalamic nucleus (VMH) Hypothalamus 230531 pAAV-AiE2150m-minBG-SYFP2...formation Cortical subplate Striatum Pallidum Hypothalamus Cerebellum Whole brain Addgene ID Plasmid Allen...Gore BB, Graybuck L, Greisman N, Haeseleer F, Halterman C, Helback O, Hockemeyer D, Huang C, Huff S, Hunker...

  11. Plant Plasmids and Resources

    Type
    Collection
    ...distributes diverse seed and other stocks of Arabidopsis thaliana and related species. The Arabidopsis Information...genetic and molecular biology data for Arabidopsis thaliana research. BAR The Bio-Analytic Resource for Plant...Database of gene expression profiles in Arabidopsis thaliana based on RNA-seq analysis. Return to top Do you...

  12. TALEN Plasmids and Kits

    Type
    Collection
    ...TALEN Kit 49042 EMM65 Bradley Bernstein and Eric Mendenhall The TALE plasmid vectors described on this page...4 (H3K4) di-methylation at the target locus ( Mendenhall et al., 2013 ). This epigenome editing was also...49044 EMM71T Golden Gate TALEN 2.0 49401 pBlue-TAL Michal Zurovec pBlue-TAL was designed for use with the...

  13. CRISPR References and Information

    Type
    Collection
    ..., C. elegans, mouse, human, rat, cow, dog, pig, Thale cress, rice (Oryza sativa), tomato, corn, monkey...pCRISPR new spacer cloning pCRISPR PDF 106.7 KB Mendenhall & Myers Mammalian: FLAG tagging endogenous proteins...tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF 47.5 KB Vosshall and Matthews CRISPR/Cas9 reagent generation; gRNA...

  14. Rett Syndrome

    Type
    Collection
    ... results in approximately half of cells expressing wild-type MECP2 and half expressing mutant MECP2 . ...Kankirawatana et al. 2006. Early progressive encephalopathy in boys and MECP2 mutations. Neurology . 67...

  15. Deisseroth INTRSECT Collection

    Type
    Collection
    ..., Deisseroth K, Carlén M, Meletis K. 2019. A hypothalamus-habenula circuit controls aversion. Mol. Psychiatry... Osten P, Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal Circuits through the Parafascicular Nucleus...window) Marcinkiewcz CA, Mazzone CM, D'Agostino G, Halladay LR, Hardaway JA, DiBerto JF, Navarro M, Burnham...

  16. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang...Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers RM. 2015. CETCh-seq: CRISPR epitope...proteins. Genome Res . 25(10):1581-9. PMID: 26355004 Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson...

  17. Distribution to Industry

    Type
    Collection
    ...agreements allow Addgene to distribute plasmids on behalf of the depositing institutions. These agreements...signatory is someone who can execute legal documents on behalf of the recipient institution. Once the MTA has ...

  19. TALEN Guide

    Type
    Collection
    ...led by Jens Boch at the Martin-Luther-University Halle-Wittenberg and Adam Bogdanove at Iowa State University...into a single construct would be technically challenging. Thanks to efforts by the Bogdanove group and...

  20. Cre-lox system

    Type
    Collection
    ...respectively. Split Cre: Cre recombinase is split in half into N and C terminal Cre fragments (NCre and CCre...s View References Lakso M, Sauer B, Mulder KL, Westphal H. 1992. Targeted Oncogene Activation by Site-...
Showing: 21 - 40 of 429 results