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... target recruitment and nicking, producing STITCHR. STITCHR can make a wide variety of genomic edits —...recombinant antibodies for Glypicans DNA editing with STITCHR Dynamic protein labeling with TurboCas Enhancer-AAV...-guided genome editing, think beyond Cas9 with STITCHR: a combined CRISPR / retrotransposon system with...added to the genome (Figure 4). Figure 4: STITCHR consists of a nicking Cas9 fused to a shortened...unlike previous integrase-based approaches. Find STITCHR plasmids here! Fell, C. W., Villiger, L., Lim,...

...greatest challenges of neuroscience.”  Hot plasmids: Twitch sensors Griesbeck’s recent contribution to the ...based calcium biosensors. Perhaps most notably, the Twitch calcium sensors, which are available at Addgene...in vivo imaging. Griesbeck explains, while these Twitch sensors are always bright, they change color as...reported in Nature Methods, the FRET changes of these Twitch sensor variants were optimized by testing various... rat hippocampal neuron cultures. The optimized Twitch sensors allowed the researchers to see tonic action...

...to the switch and exposes its ribosome binding site, allowing for the gene on the toehold switch to be ...sequence of the toehold switch. For example, Green has also developed toehold switches to detect Zika virus...amplified isothermally, and detected through a toehold switch, an RNA sensor that can be used to detect the presence...

...we discussed inducible promoters, which can be switched from a default OFF to an ON state, and how you...ll look at repressible promoters, which can be switched from a default ON to an OFF state, as well as ...plasmids, see our Tetracycline resource page. Cumate Switch Massie et. al developed the cumate-inducible gene...promoter and turning transcription OFF. The cumate switch system functions well in mammalian cells, and can..., A., Xu, Y., Warren, R. et al. The cumate gene-switch: a system for regulated expression in mammalian...

...overexpressed in cells, microtubule growth can be switched off with blue light which causes LOV2 and Zdk1...cells. These tools consist of cobalamin-binding riboswitches that bind to cobalamin-fused fluorescent dyes...dyes; binding of the cobalamin fusion to the riboswitch results in increased fluorescent signal. Prior ...find plasmids containing the cobalamin binding riboswitches used in the Riboglow platform at Addgene. ...

...your idea is viable.  Go to experts you trust and pitch what you hope to build.  Then ask for their honest...honest opinions -- this part might hurt, but if you pitch to enough people, you will find the answer to your...an impossibility at our early stage.  After each pitch we made, we considered the feedback and adjusted...viable.  This is not to say that we aren’t still pitching for feedback.  Your venture should continuously...

...derivatives of violacein. Using dCas9 as an on/off switch The multiple scRNAs all individually utilize the... can be regulated with dCas9 as a master ON/OFF switch. This will likely prove particularly useful in ...distinct programs controlled by each dCas9 regulatory switch. By no means limited to the field of metabolic ...variants can be used as regulatory ON/OFF master switches to induce multiple transcriptional programs. ...

...Xin Zhou greatly expanded that list by using photoswitchable fluorescent proteins to create enzymes that...turned the enzymes back on. Xin termed these photoswitchable enzymes Fluorescent Light-inducible Proteins...various designs, Xin has been able to develop photoswitchable Intersectin, Cdc42, Raf1, Mek1, Mek2, Cdk5,... You can learn more about Xin’s work with photoswitchable components of the ERK signaling pathway in ...

...infection. Cre or Flp dependent switches control gene expression via switches placed in your cell type of ...specialized promoters, Flp or Cre dependent gene switches, and serotype selection. Promoters and serotype...interest. Careful combination of promoters, gene switches, and/or serotype can also be used to ensure specificity...

...optogenetics plasmids such as microbial opsins and optical switch tools to activate, inhibit, and manipulate cellular...Plasmid Collection Microbial Opsin Plasmids Optical Switch Plasmids Additional Resources Optogenetics plasmids...proteins. These fusions can then be used as an optical switch to control protein interactions, dimerization, ...spectra) of different types of opsins and optical switches available in these tables, refer to Addgene's ...Eligible for Viral Packaging PI Return to top Optical Switch Plasmids Browse, sort, or search the table below...

... Tian Calcium Sensor: Twitch2B 100040 pAAV.hSyn1.Twitch2B.WPRE.SV40 Syn Twitch2B none Constitutive 1 Oliver... Oliver Griesbeck 173044 AAV-Camk2-NES-Twitch-GR CamKII Twitch none Constitutive 2 Yiyang Gong Calcium...ribo-GCaMP8 GCaMP7 GCaMP6 axon-GCaMP6 soma-GCaMP6 Twitch Yellow Calcium Sensors jYCaMP1 axon-jYCaMP1 Red...

...interest makes FLEx switch a powerful experimental tool. For more information on FLEx switch and what you can... Cre expression promotes shRNA expression. Gene Switch: These constructs contain two genes of interest...frame to allow in-frame translation of Gene B. FLEx Switch (for “flip-excision”): Also known as DIO (Double-floxed...while simultaneously turning on another. The FLEx switch system takes advantage of the orientation specificity...

...function. Adapting Toehold Switches to Detect Zika Virus The RNA-based toehold switches described in this post...binding, produce reporter proteins. These toehold switches can be used to quickly detect the presence of ...

... discussion on the way to the office or in the kitchen, while sipping an afternoon coffee in between experiments...a coffee, or simply moves away from the desk to switch off a few minutes. The host could also set aside...used and could bring back a taste of that casual kitchen chatter, ultimately sparking new ideas. Social ...

...beyond stability, adding color and even on-off switches. Stabilizing gRNA modifications  Sugar phosphate...modifications to improve stability.   On-off switches for gRNAs A major drawback of CRISPR-Cas9 technology..., E., Satyapertiwi, D., Ramel, MC., et al. Photoswitchable gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based...

...subclass specific secondary antibodies and subclassed switched R-mAbs, multiple targets could now be visualized...Fig. 2. Multiplex immunolabeling with subclass-switched recombinant antibodies in adult rat brain. Figure...Engebrecht J, Trimmer JS. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced...

... Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow...parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades...parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades...

...to control cells or organisms with the flip of a switch. You may be familiar with popular light-sensitive...activity. Researchers have designed a variety of photoswitchable elements into many other types of proteins....in the Gadella Lab devised a set of optogenetic switches for the GEFs ITSN1, TIAM1, or p63RhoGEF (which...

...including photoactivatable, photoconvertible, and photoswitchable proteins, organized by color and expression...Corinebacterium glutamicum Timothy Lu 36252 pRibo EcoHind Riboswitch Teophylline Escherichia coli , Mycobacterium ...Bertozzi , Jessica Seeliger 36251 pRibo BsaHind Riboswitch Teophylline Escherichia coli , Mycobacterium ...Carolyn Bertozzi , Jessica Seeliger 202406 pJYP1 Riboswitch Cobalamin (vitamin B12) Escherichia coli Remy...

...were a way to just make one fusion protein that switches to any color or tag you want? It sounds like magic...ticker tape" method for intracellular recordings, switching between different colored ligands at different...increasingly popular. Embrace the flexibility and switch things up for your next experiments!   References...