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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 810 results for: mak

Showing: 21 - 40 of 810 results

  1. CRISPR Plasmids - Tagging

    Type
    Collection
    ... CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed a ...alternative DSB repair pathway, instead of HDR, which makes it easy to construct a donor vector, because MMEJ...

  2. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...and improving the orthogonal translation system to make phosphorylated proteins with one or more phosphorylation...expressing recombinant phosphoproteins. These attributes make rEcoli XpS (Bacterial Strain #192872) and pSerOTS-C1...Study Protein Phosphorylation Read about GCE4All: Making Genetic Code Expansion Accessible...

  3. Bacterial Expression Systems

    Type
    Collection
    ...cell, you will need to tag it with something that makes it easy to see under the microscope. The plasmids...Fluorescent Dyes and Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent...to FRET Bimolecular Fluorescence Complementation Makes Visualizing Protein–Protein Interactions Easy Split...

  4. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...expression systems. Addgene partnered with Ginkgo to make these plasmids available to scientists in academic...Codon-optimized expression (recode) Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations...

  5. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...plasmids and backbones with high cloning potential. By making these plasmids available as cgDNA, we hope to aid...note that this does not guarantee that Addgene will make cloning grade DNA preps of this plasmid available...

  7. Antibody Guide

    Type
    Collection
    ...plasmids. Animals - Antibodies produced in animals make use of the animal’s immune system. The antigen of...Overview of application Collect and lyse samples to make proteins available. Tissue samples may need additional...Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made from plasmids...

  8. Serotype Testing AAV

    Type
    Collection
    ...purified DNA from the original plasmid that was used to make the virus. For more details, see our FAQ on What... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our ...

  9. Viral Vector Guides and Plasmids

    Type
    Collection
    ... expression. Retroviral vectors are popular for making stable cell lines or clinical use. Adenoviral Vector...BioRender.com. Wild-type viruses have one goal, which is to make more viruses. The viral genome contains only the...

  10. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...finger nucleases (ZFNs) are effective tools for making targeted mutations in zebrafish. With support ...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML et al....

  11. AAV Packaged on Request

    Type
    Collection
    ...perform comprehensive quality control processes to make sure it is ready for your experiments. Shipping ...experience, from methods to troubleshooting insights, you make research more reproducible for scientists everywhere...

  12. TALEN Guide

    Type
    Collection
    ... 18 TAL effector repeats. The kit can be used to make arrays from 12-31 repeats. Perhaps one of the most...TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt...

  13. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... Selectable Marker PI Publication Epigenetics To make targeted epigenetic modifications, researchers have... require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. ID Plasmid Gene...

  14. COVID-19 Resources

    Type
    Collection
    ...spike protein constructs for making VSV pseudo virus. Addgene blog Scientists Map the...opens in a new window) Kogiso, H., Raveau, M., Yamakawa, K., Saito, D., Ikeuchi, Y., Okazaki, T., Asano...

  15. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...of target sites that flox your genes of interest makes FLEx switch a powerful experimental tool. For more...transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid Description Gene/Insert...

  16. Luciferase Plasmid Collection

    Type
    Collection
    ...Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters, but are...a secreted version of the protein is available, making it ideal for a variety of applications. To learn...

  17. Immunology Research Plasmids and Resources

    Type
    Collection
    ... secreted, (semaphorin) 3E KIAA0331, M-SEMAH, M-SemaK, SEMAH, coll-5 SEMA3F sema domain, immunoglobulin...domain, secreted, (semaphorin) 3F SEMA-IV, SEMA4, SEMAK SEMA3G sema domain, immunoglobulin domain (Ig), ...H-SEMA-K1, H-Sema-L, JMH, MGC126692, MGC126696, SEMAK1, SEMAL SLIT1 slit homolog 1 (Drosophila) MEGF4,...

  18. Zhang Lab CRISPR Page

    Type
    Collection
    ...Targeting vector for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling 60224...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. Nature . 2015...

  19. Plan Your Experiment

    Type
    Collection
    ...setting up CRISPR experiments. We will focus on making single edits using CRISPR/Cas9 in mammalian cells...gRNA design. While there are some cases where it makes sense to manually design a gRNA, in most cases gRNA...

  20. Validated gRNA Sequences

    Type
    Collection
    ... gRNA sequence in your particular experiment, so make sure to check out the particular conditions of the...GGGGCCACTAGGGACAGGAT 72833 cut S. pyogenes 27052166 Kanemaki mmp21 D. rerio CGGAGCTGATCACTGACA 72890 cut S....
Showing: 21 - 40 of 810 results