Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 802 results for: mak

Showing: 21 - 40 of 802 results

  1. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Packaged on Request...protocols for how to use your viral prep and how to make your own preps. Recipient Instructions What to do...purified DNA from the original plasmid that was used to make the virus. In order for Addgene to provide viral...

  2. CRISPR Plasmids - Tagging

    Type
    Collection
    ... CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed a ...alternative DSB repair pathway, instead of HDR, which makes it easy to construct a donor vector, because MMEJ...

  3. Plan Your Experiment

    Type
    Collection
    ...reagents are appropriate for a given experiment. Make sure to check whether reagents are available to ...efficiency compared to NHEJ knockout. Base editors can make a limited set of mutations. Repress or Interfere...envelope plasmids provide the necessary components to make lentiviral particles (for details about lentivirus...

  4. Bacterial Expression Systems

    Type
    Collection
    ...cell, you will need to tag it with something that makes it easy to see under the microscope. The plasmids...Fluorescent Dyes and Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent...to FRET Bimolecular Fluorescence Complementation Makes Visualizing Protein–Protein Interactions Easy Split...

  5. Luciferase Plasmid Collection

    Type
    Collection
    ... stable, making time course experiments possible, but its short luminescence half-life makes it too dim...Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters. However...

  6. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...expression systems. Addgene has partnered with Ginkgo to make these plasmids available to scientists in academic...Codon-optimized expression (recode) Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations...

  7. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...plasmids and backbones with high cloning potential. By making these plasmids available as cgDNA, we hope to aid...note that this does not guarantee that Addgene will make cloning grade DNA preps of this plasmid available...

  8. Serotype Testing AAV

    Type
    Collection
    ...purified DNA from the original plasmid that was used to make the virus. For more details, see our FAQ on What... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our ...

  10. Antibody Guide

    Type
    Collection
    ...plasmids. Animals - Antibodies produced in animals make use of the animal’s immune system. The antigen of...Overview of application Collect and lyse samples to make proteins available. Tissue samples may need additional...Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made from plasmids...

  11. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...finger nucleases (ZFNs) are effective tools for making targeted mutations in zebrafish. With support ...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML et al....

  12. AAV Packaged on Request

    Type
    Collection
    ...perform comprehensive quality control processes to make sure it is ready for your experiments. Shipping ...experience, from methods to troubleshooting insights, you make research more reproducible for scientists everywhere...

  13. TALEN Guide

    Type
    Collection
    ... 18 TAL effector repeats. The kit can be used to make arrays from 12-31 repeats. Perhaps one of the most...TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt...

  14. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... Selectable Marker PI Publication Epigenetics To make targeted epigenetic modifications, researchers have... require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. ID Plasmid Gene...

  15. Cre-lox system

    Type
    Collection
    ...of target sites that flox your genes of interest makes FLEx switch a powerful experimental tool. For more...interest by removing neoR and stop sequence; used for making transgenic mice. Plasmid 15037 also contains IRES-EGFP...transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid/Article Description...

  16. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...and improving the orthogonal translation system to make singly or multiply phosphorylated proteins (Pirman...expressing recombinant phosphoproteins. These attributes make rEcoli XpS ( 192872 ) and pSerOTS-C1* ( 188537 )...

  17. Immunology Research Plasmids and Resources

    Type
    Collection
    ... secreted, (semaphorin) 3E KIAA0331, M-SEMAH, M-SemaK, SEMAH, coll-5 SEMA3F sema domain, immunoglobulin...domain, secreted, (semaphorin) 3F SEMA-IV, SEMA4, SEMAK SEMA3G sema domain, immunoglobulin domain (Ig), ...H-SEMA-K1, H-Sema-L, JMH, MGC126692, MGC126696, SEMAK1, SEMAL SLIT1 slit homolog 1 (Drosophila) MEGF4,...

  18. Zhang Lab CRISPR Page

    Type
    Collection
    ...Targeting vector for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling 60224...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. Nature . 2015...

  19. Validated gRNA Sequences

    Type
    Collection
    ... gRNA sequence in your particular experiment, so make sure to check out the particular conditions of the...GGGGCCACTAGGGACAGGAT 72833 cut S. pyogenes 27052166 Kanemaki mmp21 D. rerio CGGAGCTGATCACTGACA 72890 cut S....

  20. Antibodies

    Type
    Collection
    ...transparency and accessibility in research and therefore makes the sequences of these affinity reagents available...
Showing: 21 - 40 of 802 results