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We narrowed to 457 results for: nsf

Showing: 21 - 40 of 457 results

  1. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...postdocs in science, engineering and health from the NSF’s 2014 Graduate Student Survey data). Most institutions...populations potentially from 1 to 5761, according to the NSF data) and therefore the numbers of postdocs at different...implies. These 5 organizations, according to the NSF data, account for 18% of the postdoctoral workforce...

  2. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...reduce barriers to implementation. Funded by the NSF (with Co-PIs Jessica Bell, Lisa Gentile, Ellis Bell...Hampshire. He mentors undergraduates conducting NIH- and NSF-funded research on planarian flatworms both in and...). But is it possible to bring the kinds of transformative educational experiences afforded to those fortunate...NADH production.   CUREs are beginning to transform undergraduate education in the sciences by making...

  5. General Transfection

    Type
    Protocol
    ...RT. Carefully transfer the transfection mix to your HEK293T cells. Add the transfection mix dropwise, ...general method for transfecting mammalian cells using linear polyethylenimine. Transfections allow for transient... viral production) Day 1: Transfect Cells Day 2 (am): 18 h post transfection - Remove media, replace with...protocol to transfect mammalian cells with your plasmid of interest. Protocols... Protocols General Transfection General...General Transfection You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service...adapted for different cell lines and different transfection reagents. Workflow Timeline Day 0: Seed HEK293T...

  6. Protocol - Bacterial Transformation

    Type
    Protocol
    ...Introduction Transformation is the process by which foreign DNA is introduced into a cell. Transformation of bacteria...prepared for optimal transformation efficiencies upon thawing. For the highest transformation efficiency, we...time when carrying out transformations? If you are not concerned with transformation efficiency (such as ... 20-30 mins) I get very few if any transformants when transforming large plasmids (>10 kb) or BACs, what...Learn how to transform E. coli with your plasmid of interest. Protocols... Protocols Bacterial Transformation Bacterial...Bacterial Transformation You may also like... Plasmid Cloning by Restriction Enzyme Digest Restriction Digest...

  7. Transfection for Recombinant Antibodies

    Type
    Protocol
    ... to proceed with transfection. Transfect the flask containing 108 mL cells: Transfer 6 mL of BCD TFX into...General Transfection Protocol Recombinant Antibody Purification Protocol Introduction Transfections allow...using Polyethylenimine Max as a transfection reagent. After transfection and expression, the recombinant...Section 2: Transfection Check the cell density and viability: Using a 5 mL pipette, transfer 0.5 mL of ...feeding schedule: Thursday: Transfect cells. Friday (24 h post-transfection): Add 4% BCD Feed. Monday (...post-transfection): Add valproic acid to 3.75 mM, add 4% BCD Feed. Tuesday (120 h post-transfection): Add...Wednesday (144 h post-transfection): Add 4% BCD Feed. Thursday (168 h post-transfection): Harvest antibody...

  8. Open Enzyme Collection

    Type
    Collection
    ...pOpen-EcoRIM M.EcoRI DNA methyltransferase 165517 pOpen-Eco31IA Eco31IA DNA methyltransferase 165518 pOpen-NotIM...pOpen-NotIM M.NotI DNA methyltransferase 165527 pOpen-Eco31IB Eco31IB DNA methyltransferase 165536 pOpen-PstIM...pOpen-PstIM M.PstI DNA methyltransferase 165538 pOpen-SfiIM M.SfiI 165538 DNA methyltransferase 165547 pOpen-SapIM1...pOpen-SapIM1 M1.SapI DNA methyltransferase 165557 pOpen-SapIM2 M2.SapI DNA methyltransferase 165558 pOpen-EcoRVM...pOpen-EcoRVM M.EcoRV DNA methyltransferase 165577 pOpen-XbaIM M.XbaI DNA methyltransferase 165578 pOpen-NcoIM ...into an expression vector of their choice and transforming into E. coli bacteria. This can help reduce ...an expression vector of their choice and then transform into E. coli for expression and purification (...

  9. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...another transfection method such as cationic liposome-based transfection. Optimize transfection conditions...ligation to occur in one reaction (see step 3.2 ). Transform 10 μl of DH5α E. coli cells with 1 μl of reaction...Perform maxi-prep for each CRISPR/Cas9 construct. Transfecting CRISPRs into Cells of Interest NOTE: This protocol...which is used for MEL cells. However, transient transfection of CRISPR/Cas9 plasmids may be successfully ...types using preferred culture conditions and transfection strategies for each cell type. While MEL cells... before attempting genome editing. Immediately transfer solution from cuvette into 1 ml of culture media...Minimize the time between electroporation and transferring the solution into media to enhance cell viability...

  10. Lentivirus Plasmids

    Type
    Collection
    ...Addgene lentiviral packaging, envelope, and transfer plasmids...Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses...encodes the viral genome (typically called the transfer plasmid ). This table lists common lentiviral ... Contains Rev; can be used with 3rd generation transfer plasmids along with additional packaging components...Contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev Trono...and Tat; can be used with 2nd and 3rd generation transfer plasmids Trono 12263 pCMV delta R8.2 2nd Packaging...and Tat; can be used with 2nd and 3rd generation transfer plasmids Trono 12259 pMD2.G N/A Envelope VSV-G-expressing...

  11. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral Transfer Vectors Resources Kazuhiro Oka and...lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this set of...packaging these transfer vectors and generating new viral vectors. Download the Lentivirus Transfer Vector Production...from the PGK promoter Adenoviral Transfer Vectors The adenoviral transfer vectors contained within this ... of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP...Flp recombinases. Genes can be cloned into these transfer vectors using shuttle vectors pICPIS-EF1 (Addgene...

  12. Immunology Research Plasmids and Resources

    Type
    Collection
    ... latent transforming growth factor beta binding protein 1 MGC163161 LTBP2 latent transforming growth factor...thyroglobulin AITD3, TGN TGFA transforming growth factor, alpha TFGA TGFB1 transforming growth factor, beta 1 ...TGFbeta TGFB2 transforming growth factor, beta 2 MGC116892, TGF-beta2 TGFB3 transforming growth factor...containing) transforming protein 2 SCK, SHCB, SLI SHC3 SHC (Src homology 2 domain containing) transforming protein...MGC138230 TGFB1 transforming growth factor, beta 1 CED, DPD1, TGFB, TGFbeta TGFB2 transforming growth factor...TGF-beta2 TGFB3 transforming growth factor, beta 3 ARVD, FLJ16571, TGF-beta3 TGFBR1 transforming growth factor...protein 2 C14orf141, LTBP3, MSTP031 LTBP3 latent transforming growth factor beta binding protein 3 DKFZp586M2123...

  13. Luciferase Plasmid Collection

    Type
    Collection
    ...Bioluminescence Resonance Energy Transfer (BRET) Similar to FRET, energy transfer from the luciferase reaction...white screening can be used for screening of transformants in cloning process. Lynne Postovit 64034 pGL4Luc-RLuc...constitutive promoter can be used to monitor transfection or viral infection efficiency, to study changes... Renilla luciferase is used to monitor viral transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623... expressing firefly luc for monitoring plant transfection. Daniel Voytas 133312 pMKV057 Firefly AtUbi10...and IPT, with firefly luc for monitoring plant transfection. Daniel Voytas 181934 pC0037 Gaussia, Cypridina...

  14. Plan Your Experiment

    Type
    Collection
    ...efficiently transfect. However, note that this method is most useful for cell lines that you can transfect at ...CRISPR system into mammalian cells is to directly transfect expression plasmids encoding your Cas protein ...gRNA, which you generally perform by chemical transfection or electroporation. Expression can be transient...Lentiviral vectors are a good option for difficult-to-transfect cells. You can direct expression to specific cell...These methods are more transient than plasmid transfection or viral vectors, reducing the risk of accumulating...method is helpful in cells that are difficult to transfect, transduce, or may not express common promoters...

  15. Antibody Production

    Type
    Collection
    ...expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding the heavy and light... The fluorescence pattern is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections...extracted from cells, separated by SDS-PAGE, transferred to a membrane, and labeled with the antibody ...antibody. Protein expression is compared to an untransfected control and the protein size is confirmed. Residual...contain low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified...

  16. Distribution to Industry

    Type
    Collection
    ... Material Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement...agreement (MTA), including transfers to for-profit entities. The MTA is a legal agreement between the recipient... the depositing institution(s). It governs the transfer of biological materials and intellectual property...Please see the Sample Agreements on our Technology Transfer Information page for more information. To learn...

  17. AAV Packaged on Request

    Type
    Collection
    ...of the applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the ...service lets scientists choose from over 4,000 transfer plasmids to be packaged in their choice of seven...chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids in our repository are eligible for this...several important steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation...

  18. Retrovirus Plasmids

    Type
    Collection
    ...Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want a retroviral...Addgene, including popular packaging, envelope, and transfer plasmids. Viral...of interest? Search our database for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description...

  19. Plant Plasmids and Resources

    Type
    Collection
    ...design of synthetic operons for chloroplast transformation and metabolic engineering in plants (including...set of functional parts for assembling plant transformation vectors - Daniel Voytas. Transcription Factors...Agrobacterium-mediated transient expression or plant transformation - Silin Zhong. RNAi The following collections...Editors Save Time with Transient Plant Leaf Transformations RNA Interference in Plant Biology: New Tools...

  20. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...resonance energy transfer, or FRET , is a process by which energy is non-radiatively transferred from an excited...excited donor fluorophore to an acceptor. Since the transfer of energy does not occur by emission of a photon...FRET experiments. The efficiency of the energy transfer is measured using fluorescence microscopy by exciting...
Showing: 21 - 40 of 457 results