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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 303 results for: rigi

Showing: 21 - 40 of 303 results

  2. TALEN Guide

    Type
    Collection
    ...TALEN Kits CRISPR Plasmids Zinc Finger Consortium Originally published in Addgene's December 2011 Newsletter...

  3. Arf GTPase Family

    Type
    Collection
    ..., & Ettema, T. J. (2016). Tracing the archaeal origins of Eukaryotic Membrane-Trafficking System building...

  4. Zebrafish Plasmid Collection

    Type
    Collection
    ...useful tools for studying cells that share clonal origin in zebrafish can be found below: Optimized QF-binary...

  5. COVID-19 Resources

    Type
    Collection
    ...Holmes, E. C., & Garry, R. F. (2020). The proximal origin of SARS-CoV-2 . Nature medicine, 26 (4), 450–452...

  8. Weighing Reagents Protocol

    Type
    Protocol
    ...much material, don’t put any extra back into the original container. Dispose of the excess material into...

  11. Plasmids for Stem Cell Research

    Type
    Collection
    ...40. Hochedlinger MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS...

  12. Genetic Code Expansion

    Type
    Collection
    ...of the cognate tRNA gene. ID Plasmid Synthetase Origin ncAA Expression Codon PI 31186 pEVOL-pAzF p-azidohenylalanine...

  14. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...situ of Regulatory Elements: The original CAPTURE method The original CAPTURE method requires the creation...Originally published Dec 7, 2017 and updated Jul 2, 2020. Promoters may be the star of gene regulation...Purification in situ of Regulatory Elements) method. The original CAPTURE method was published in 2017 and addressed...by PCR or deep sequencing. A drawback of the original CAPTURE method is that a new cell line has to ... of the CAPTURE 2.0 system To improve upon the original CAPTURE method, the Xu Lab made the following ...plasmids for CAPTURE 2.0! When compared to the original CAPTURE, CAPTURE 2.0 had a ~14-fold increase in... Comparing CAPTURE and CAPTURE 2.0.     Original CAPTURE CAPTURE 2.0 Biotinylation enzyme BirA...

  15. What the HEK?

    Type
    Blog Post
    ...is difficult to determine its true tissue origin. Originally, it was assumed HEKs were a common cell type...wondered where human embryonic kidney (HEK) cells originated from? Or why they are such a commonly used cell...the nomenclature we use today: HEK-293. The exact origin of the fetal tissue which HEKs were isolated from...line have determined it is most likely adrenal in origin. (Anatomy refresher: the adrenal gland is next ... them to replicate viral plasmids with the SV40 origin of replication. To generate a virus, you need only...recombinant proteins are necessary, HEKs are human in origin, so they eliminate non-human PTMs which is a concern...human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. (2002) (8):869-71. DOI...

  16. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ... the origin of replication and the replication control machinery (Need a refresher on the Origin of Replication...Replication? See our Plasmids 101: Origin of Replication blog).  The replication problem Plasmids with... directly inhibit replication by binding to the origin of replication and blocking replication machinery...problem. Iterons are repeated sequences within the origin of replication and are required for replication...machinery. However, when plasmids have the same origin of replication, the negative regulation system ...our Plasmids 101 blog posts Learn more about the origin of replication Find blog posts about plasmid cloning...

  17. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...expression levels; expression vector cannot have p15A origin of replication F- ompT lon hsdSB (rB- mB-) gal... than pLysS; expression vector cannot have p15A origin of replication F- ompT lon hsdSB (rB- mB-) gal... in E. coli. Expression vector cannot have p15A origin of replication F- ompT hsdSB(rB- mB-) gal dcm (...body formation. Expression vector cannot have p15A origin of replication fhuA2 [lon] ompT gal (λ DE3) [...pREP4 plasmid). Expression vector cannot have p15A origin of replication F-, Φ80ΔlacM15, thi, lac-, mtl-,...resistance cassette for positive selection and a p15A origin of replication, making it incompatible with other...backbones.   Our Most Popular Plasmids 101 Posts: Origin of Replication The Promoter Region – Let's Go! ...

  18. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...look at the origins of the system and explore advances that have happened since our original 2018 blog ...This post was originally written by Tyler Ford in 2018. It was updated by guest blogger Abhi Aggarwal...blog post. The origins of iGluSnFR   iGluSnFR was developed in 2013 by Loren Looger’s lab at HHMI Janelia...through different C-terminal anchoring domains The original iGluSnFR in the pMinDisplay backbone is fused ... on the extracellular side. In addition to the original PDGFR TMD, the researchers introduce iGluSnFR3...

  19. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ...this is where most of our conference room names originated from. Let’s take a look at some of the stories... SB transposon system including those from the original publication, Ivics et al 1997. Spaghetti Squash...may think that a gene called "spaghetti squash" originated from the squash itself, but think again. Spaghetti...spaghetti? I actually couldn’t find the specific origins of the name but some of our Addgenies had an idea...Azplazu and Frasch, 1993). I could not find the exact origin of this gene name but the larval gut of bagpipe...learned some fun facts about these genes and the origins of their names!    References Azpiazu, Natalia...

  20. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...to the same extent. As described in our previous Origin of Replication post, DNA replication is initiated...4.6 million base pairs long and contains a single origin of replication (oriC), where initiation begins..... Thanks, stringent regulation! This post was originally written by Jason Niehaus in December 2015 and...., Dyson, K., Rao, P., & Leonard, A. C. (2018). Origin recognition is the predominant role for DnaA-ATP...Han, E., Paulsson, J., & Chattoraj, D. K. (2001). Origin pairing (‘handcuffing’) as a mode of negative control...Resources at Addgene Read our blog post on the Origin of Replication Browse other Plasmids 101 Posts ...
Showing: 21 - 40 of 303 results