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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 265 results for: ttl

Showing: 21 - 40 of 265 results

  1. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    ...mug or reusable water bottle, you’d probably bring in a mug or reusable water bottle instead of buying yourself...contributors to the sustainability problem, how can one little change make a difference? Individual changes to...yourself a big stack of plastic cups. By initiating little changes in ourselves, we can both influence others...

  2. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ... discarded.  Seeding Labs does an amazing job shuttling unwanted lab supplies to places that really need...one point I started to become very aware of how little care I took with my discards and to be mindful ...the lab materials I was using.  I started to do little things to save reagents and money.  This was the... for smaller and smaller gel apparatus until I settled on using glass slides. With just the right amount...

  3. CRISPR Guide

    Type
    Collection
    ...Chavez, A., Scheiman, J., Vora, S., Pruitt, B. W., Tuttle, M., Iyer, E. P. R., Lin, S., Kiani, S., Guzman...

  4. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...color throughout the day) and doing this with very little money. So when did inspiration strike us so hard...started to entertain other options. Keira had it a little better. As a Master’s student, she had less pressure...help from SynBio Axlr8r We limped along with very little traction for quite a while, and even had some serious...on one another to celebrate good news or share a bottle of wine after a rough day, and we have great partners...

  5. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...solutions they promote help motivate you to spend a little time advocating for science and getting yourself...loved - there were booths for children to learn little things about science. We need to keep encouraging... and science education. Photo cred Alina Chan. Seattle, Washington, USA 47.6062°N 122.3321°W– Justin Sherman... professionals and supporters. I'm raising some little scientists (as you can see in the picture) and ...

  6. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ..., and ColE2 plasmids) as cell division slows (Kittleson et al., 2011). Segall-Shapiro et al. showed that...the accumulation of toxic intermediates or the shuttling of the intermediates into other pathways. In cases...biotechnology 36.4 (2018): 352. PubMed PMID: 29553576. 2. Kittleson, Joshua T., Sherine Cheung, and JChristopher Anderson...

  7. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...options to choose from.  Transfer Transfers are a little trickier than gels and may require multiple runs...your protein is over-transferring! If you see very little signal, but you know you loaded a good amount of...protein-based buffers, so here’s a quick tip: Keep a bottle of chemical buffer around, which works well and...stripping* Use a harsher stripping buffer Too little signal Large proteins: Incomplete transfer ...

  8. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...incorporated at a precise genomic location, with little to no room for error. It’s no surprise then that...Prolonged cell cycle arrest can lead to apoptosis in as little as 10 hours, so read up on the timing of this approach...factors were loaded onto the break, potentially shuttling repair down a different pathway (Charpentier et...

  9. Finding Your Science Policy Path

    Type
    Blog Post
    ...policy, there is often discussion of “big P” and “little p” policy.  “Big P” policy is what you might typically...activities, formal lobbying, or strategy setting.  The “little p” policy is non-governmental work happening at...areas. Whether you’re interested in “big P” or “little P” policy, the possibilities are endless leading...

  10. Technical Design of a Western Blot

    Type
    Blog Post
    ... which are produced from plasmids and have very little batch-to-batch variation. Confirmation of sequence...specific protein modifications, we recommend doing a little more research into gel chemistry and/or specialty...pre-wetting and has lower background than PVDF. It is a little quicker to use, with high binding capacity, though...

  12. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...find out how we can serve them better. Here’s a little bit of what we learned. Check Out Our Microbiology...temporary tattoos on the spot with our trusty spray bottles! We also raffled off one of our highly sought-after...

  13. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...experiment, the Morris lab identified a novel gene, Mettl7a1, whose expression helped coax cells towards successful...uncharacterized putative methyltransferase. Adding Mettl7a1 to the standard Foxa1-Hnf4α retroviral overexpression...

  14. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...neuropsychology) and from areas in which I knew very little. Diving into subjects like risk perception, scientific... measure how individuals respond to issues of unsettled science. Understanding just how the public utilizes...

  17. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...reasoning,” the catchall term for a range of neat little tricks our minds perform to avoid cognitive dissonance...10.1073/pnas.1704882114 Kahan, D., Peters, E., Wittlin, M., Slovic, P., Ouellette, L. L., Braman, D., ...

  18. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ... the Addgene lab. In this post, I’ll tell you a little bit about what I learned from a New England Biolabs...CosMCPrep system for this, but thought we could learn a little bit more about high throughput DNA prepping by ...

  19. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...monitor these features were lacking until Roberta Gottlieb and Zhen Yan labs developed MitoTimer plasmids... can be imported to the mitochondrial matrix. Gottlieb’s lab has used an inducible MitoTimer reporter ... to orange) protein. Image courtesy of Roberta Gottlieb. Laker et al., J Biol Chem 2014 Apr 25;289...

  20. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ... Possible Adenovirus Remains Episomal (Little Risk of Insertional Mutagenesis) High Gene Expression...High Immunogenecity AAV Remains Episomal (Little Risk of Mutagenesis) Very Low Immunogenicity Infects...
Showing: 21 - 40 of 265 results