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We narrowed to 99 results for: ubc

Showing: 21 - 40 of 99 results

  1. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...transcribed into cDNA fragments that are efficiently subcloned into the phagemid pDX_init plasmid, using the ...sybody pools from the phage display screens are subcloned (using FX cloning) into the expression vector ...for identification. All unique sybodies can be subcloned into pBXNPH3 or pBXNPHM3 expression plasmids for...

  2. MXS Chaining

    Type
    Blog Post
    ...generated with MXS-chaining were used to label subcellular structures in live cells. Four individual expression...detection of each fluorophore at the respective subcellular structure (Figure 1). Here, MXS Chaining gave...Excitation/emission maxima Tethering partner Subcellular localization 1 TagBFP 399nm/ 456nm histone ...

  3. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ..., it cannot be used for subcellular analysis such as morphology or subcellular localization studies. For...raised in the same host species, of the same Ig subclass, and conjugated to the same fluorochrome as your...

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... shown to be useful tools for light-mediated subcellular localization in mammalian cell culture and reversible...were used in a variety of experiments including subcellular visualization of 6 different organelles, a cell...dynamics. These fusion proteins have been used as subcellular markers to visualize the dynamics and interactions...proteins can act as markers for visualization of subcellular structure, for performing live-cell imaging experiments...

  6. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...transgenes via increased nuclear export. LTR Subcomponents: -U3 -R -U5 LTR; Long terminal repeats; U3...bigger than ∼3 kb are packaged less efficiently. Subcomponents: U3; Unique 3'; region at the 3' end of viral...

  7. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...be from the same host species, have the same Ig subclass, and be in the same fluorophore (e.g., if you ...staining for anti-mouse Ly6C in APC with the IgG2c subclass, you will use the APC IgG2c isotype control). ...

  8. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...secondary antibodies generated against isotype subclasses, such as mouse IgG2 or IgG4. This can be important...secondary antibodies which recognize specific isotype subclasses of primary antibodies can increase the possible...

  9. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... cells (e.g., a neuron subtype) and specific subcellular compartments for measurements. All of the sensors...Localization The calcium concentrations in different subcellular compartments can vary greatly. Additionally, ...

  10. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...lysosome-associated membrane protein, and following its subcellular localization overtime (Subach et al.) Monitor...particular biological event (trafficking to a subcellular location, start of gene expression, development...

  11. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ... be included in a single PCR, for example for subcloning genes from different templates. All PCRs are ...can be included at the 5’ end of either primer. Subcloning - To move a gene from one vector to another simply...

  14. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...microtubule-destabilizing drugs are impossible to target to specific subcellular regions, laser ablation can damage other cellular...reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular...

  16. Antibody Guide

    Type
    Collection
    ...antibodies can be isotype-specific or even isotype subclass-specific. This specificity can be used to ensure...Isotype Description Configuration and Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears...should never be stored below 4 °C. Certain isotype subclasses, such as IgG3, aggregate after thawing and therefore...different species of origin, isotypes, and isotype subclasses of a primary antibody, which becomes the limiting...? You may need to buy isotype-specific or even subclass-specific secondary antibodies to allow for differentiation...

  17. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...electron microscopy (EM) tag providing superior subcellular localization of tagged proteins compared to traditional...proteins of interest for EM imaging, or targeted to subcellular regions or protein complexes for proteomic mapping...

  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...blue/green) Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA. Nat Commun. 2014 Jun ...genetically encoded sensors for population and subcellular imaging of cAMP in vivo. Nat Methods. 2022 Nov...FRET-based cGMP biosensor SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation. Cell Rep...for mitochondria or cytosol Hypoxia triggers subcellular compartmental redox signaling in vascular smooth... Ultrafast fluorescent voltage sensor ASAP2f Subcellular Imaging of Voltage and Calcium Signals Reveals...

  19. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron,109(9), 1449–1464.e13... P. (2021). Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex...
Showing: 21 - 40 of 99 results