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  1. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ... by even splashier news: Jiahao Sha of Nanjing Medical University had successfully used the CRISPR/Cas9... Times feature when he said, “All of this has basically happened in a year. It’s incredible.” Looking...

  3. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...SITN), we recently sponsored a first-time event called DayCon. DayCon is a one-day conference aimed at...Bio-Medley) to showcase SITN’s strength in the biomedical sciences and decided to offer a varied format...

  4. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...demonstrated in a report published in The Journal of Biological Engineering in March is fused to a translational...that she hopes will change the intracellular localization of RNA. “You can think of changing bases to ...

  6. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...what is going on and can contribute. Members periodically break, go exploring outside the team, and bring... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...

  9. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ...communication student group and get more involved with your local community. What do Vini and Amy say is the quickest... can grab a beer and chat about their work at a local bar. It doesn't have to be crazy complicated! Listen...

  10. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...there, you might have even seen me bust a move to “Call Me Maybe.” After cocktail hour, we feasted on sliders...portion of the entire night was when Melina and Ken called their parents up to the podium to be recognized...

  13. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...relationships as part of the job application process can dramatically increase the chances of being seriously considered...you are qualified and why you are interested specifically in the position. We frequently receive clearly...

  16. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ... (ADCD) Antibodies can bind to a pathogen and physically prevent it from entering a cell, a non-Fc-dependent...antibodies on a pathogen activates the immune system's classical complement pathway, starting a cascade of events...

  17. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...throughout an organism’s genome. These reporters have historically been used extensively in fly, mouse, and yeast.... These are effective tools but neglect some biologically relevant situations. Tissue-specific genes...

  18. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...could be used in high throughput screening for agrochemical compounds that have the potential to regulate...and AAVrg vectors. New red-shifted INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments...

  19. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...capsid other than AAV2. This production strategy is called cross-packaging.  Capsid (cap) gene The cap gene... of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248–265 . https://doi.org/10.1016...

  20. Fostering communication in remote collaborations

    Type
    Blog Post
    ...virtual face-to-face meetings as opposed to phone calls most closely replicates how you’d interact with ...investigate my protein of interest in a French clinical cohort, neither of which I would have been able...

  21. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...host range and copy number of the plasmid) and typically include a gene that is advantageous for survival...Plasmids 101: Luciferase Monitor the level of a chemical in a particular environment Produce enzymes that...

  24. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...blogosphere is to read and comment on their blogs. 5)   Local science museums are a great place to find opportunities...Science Communication Program at the University of California, Santa Cruz, has been turning scientists into...

  25. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...How else can antibiotics be used in the lab? Historically, antibiotics have also been used to disrupt ...cloning. In these instances, the cloning vector typically has two separate resistance cassettes and your...

  26. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...(CellTagD13), which is when the iEP phenotype typically emerges. Every three days, a sample of cells was...conversion, but CellTag could be used to study other biological processes where cells are undergoing identity...

  29. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...distribution. I don’t have space in this blog to call out every Addgene Team because they’re all doing...requests for scientists doing almost every type of biomedical science research. Our work accelerates and enhances...

  30. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ... sites in the ITRs. Regular Sanger sequencing typically fails to sequence through ITRs due to their secondary...of lox sites which are part of a dual lox system called FLEX or DIO. Lox sites are recognized by the recombinase...

  31. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...Randolph-Habecker J (2013). A Flexible Mouse-On-Mouse Immunohistochemical Staining Technique Adaptable to Biotin-Free...Tuson JR, Pascoe EW, Jacob DA (1990) A novel immunohistochemical technique for demonstration of specific binding...

  32. Plasmid Cloning by PCR

    Type
    Blog Post
    ...analyzing the gene’s function. The process is shown graphically in the following figure, in which we add EcoRI...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...

  34. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...type; however, their usage has been limited by technical problems. For instance, it’s much more difficult...presence of the CPP may alter protein function or localization. Thus, the usefulness of CPPs must be evaluated...

  35. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...mentoring meetings a priority. Schedule meetings or calls regularly and don’t cancel, so that you drive progress...

  37. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...aqueous samples might be affected by dry ice, and specifically how dry ice can affect virus from Addgene. ...never stored on dry ice/exposed to CO2 (Fig. 5). Basically, the CO2 goes in and the CO2 goes out. Conclusions...

  38. Components of CRISPR/Cas9

    Type
    Blog Post
    ... to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist...arrays, they did not know their functions and simply called the repeated elements “direct repeats” and the ...

  39. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ... a downregulation of signal, though it’s hard to call from this plot. The red (bottom) peak overlaps with...Research Assistant in Arlene Sharpe’s lab at Harvard Medical School.   More resources on the Addgene blog Antibodies...

  40. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...transfer plasmid sequences into the host genome. Typically, it is the sequences between and including the...pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.    Frequently asked questions...
Showing: 761 - 800 of 1009 results