We narrowed to 795 results for: Tec;
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  TypeBlog Post...central nervous system (CNS). To address this technological gap, a trio of recent papers describe the development...
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  What's Your Organism? Expanding Genomic Tools via the NSF EDGE ProgramTypeBlog Post...there are over 1,000 species. No transformation techniques are available yet. Polistes (Amy Toth) These ...
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  Supporting Reproducibility with a Connected ELNTypeBlog Post...Addgene.org Find protocols of molecular biology techniques Find plasmids at Addgene Make your work more ...
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  Transferable Skills Guide: CreativityTypeBlog Post...along. In my own career, my team has used these techniques to optimize our lab space, our protocols and ...
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  Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)TypeBlog Post...glycerol stocks When your lab adopts the MoClo technology, you will quickly go through your plasmid stocks...
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  Twenty Years of Sharing: Addgene's Viral Vector ServiceTypeBlog Post...J., & Fan, M. (2019). Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation...
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  Plasmids 101: Stringent Regulation of ReplicationTypeBlog Post...Bacteria. In Fundamental Aspects of DNA Replication. IntechOpen. https://doi.org/10.5772/19313 References Asano...
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  Tips for Writing a Good Cover LetterTypeBlog Post...writing are key skills. Other fields, often highly technical or skills-based ones, may not use them very often...
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  An Inside Look into Addgene's Dev TeamTypeBlog Post...ourselves. We are also able to consider different technical approaches that may come up during discussion....
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  Using Video to Share Your Science: We Share ScienceTypeBlog Post... American Society for Information Science and Technology 60.4 (2009): 858-862. 2. Van Noorden, Richard...
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  Transferable Skills Guide: Time ManagementTypeBlog Post...already started coming up with time management techniques of your own, but if you ever find yourself in...
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  Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right ConnectionTypeBlog Post...artifacts. This is an important downside of the technology and should always be considered when interpreting...
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  Transferable Skills Guide: Conflict ResolutionTypeBlog Post...set of transferable skills, coupled with your technical skills, can open new career opportunities for ...
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  Filming Science Videos in the Age of Social DistancingTypeBlog Post... at a 7-inch wireless monitor. Wireless video technology has gotten very impressive, but due to costs ...
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  Treating Muscular Dystrophy with CRISPR Gene EditingTypeBlog Post...gene editing, but given the speed at which the technology is advancing, precision editing treatments may...
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  Easi-CRISPR: Generating Knock-In and Conditional Mouse ModelsTypeBlog Post...2.5x more animals than i-GONAD and is a more technically difficult process. Overall, the main drawback...
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  Hot Plasmids: Spring 2025TypeBlog Post...living cells and organisms with TurboID. Nature Biotechnology, 36(9), 880–887. https://doi.org/10.1038/nbt...
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  Plasmids 101: Restriction CloningTypeBlog Post...light can damage DNA and that proper personal protective equipment should be worn when visualizing using...
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  A Guide to Getting Started in Undergrad ResearchTypeBlog Post.... Can lead to an independent project. Lab technician / Research assistant Typically has a Master's...
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  Custom CRISPR Screens & the Green Listed SoftwareTypeBlog Post... off-target effects of CRISPR-Cas9." Nature biotechnology 34.2 (2016): 184-191. PubMed PMID: 26780180....
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  Delivery Methods for Generating iPSCsTypeBlog Post... following synthetic modifications are used to protect the RNAs from cellular defenses against ssRNA: ...
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  CRISPR GuideTypeGuide... range of genome engineering techniques . Collectively, these techniques are referred to as CRISPR ( C...molecular detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection platform... to SHERLOCKv2 to detect multiple targets. For example, Cas12 can be added to detect the presence of infectious...have an interesting adaptive immune response that detects and eliminates foreign DNA, which scientists have...egularly I nterspaced S hort P alindromic R epeat) technologies. Before CRISPR, genome engineering approaches...CRISPR one of the most scalable genome editing technologies, making CRISPR perfect for genome-wide screens...CRISPR systems that bacteria and archaea use to protect against these foreign nucleic acids. Acr family...
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  Antibody GuideTypeGuide...amplification through the indirect detection method. In the direct detection method, the signaling molecule.... Figure 3: A western blot detecting proteins using the indirect detection method, with a secondary conjugated...conjugated, it is often called the detection antibody. If using an indirect detection method, add a secondary antibody...microscopy techniques used, so be sure to read materials specific to your assay. Most IF techniques use a ...terms, summarize antibody production and storage techniques, and explain common applications. Science...secretions (e.g. tears, saliva, mucus) where it protects mucosal membranes Dimer with a valency of 4 IgA1...function is unknown Monomer with a valency of 2 IgE Protects against parasites and is responsible for driving...
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  Molecular Biology ReferenceTypeGuide...acids. Plasmids and Recombinant DNA Technology Chemistry techniques enable the isolation and purification...biology resources, including: Molecular Cloning Techniques Promoters Sequencing Primers Origins of Molecular...specific DNA sequence. The field of recombinant DNA technology has overcome this obstacle by enabling the preparation...purification) and manipulation. With current cloning technology, it is easy to create and modify plasmids containing...editing is most commonly accomplished using CRISPR technology. CRISPR is composed of a DNA endonuclease and...plasmids used in the field of recombinant DNA technology have been optimized for studying and manipulating...methods, check out our guide on molecular cloning techniques . Common Bacteria Strains for Propagating Plasmids...
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  Optogenetics GuideTypeGuide... The tools and technologies developed for optogenetics research utilize light to detect, measure, and ... when combined with site specific recombinase technology like Cre-lox, which allows you to express an ...targets into large, optically assembled protein architectures in living mammalian cells. LITE Two-hybrid system... Schnitzer MJ. 2006. Next-generation optical technologies for illuminating genetically targeted brain ...
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  Science GuidesTypeGuide... The tools and technologies developed for optogenetics research utilize light to detect, measure, and ...of guides that describe how popular scientific techniques work Science...interest. Read More Chemogenetics Chemogenetics is a technique that uses genetically engineered receptors to ...
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  Plan Your ExperimentTypeGuide...editing. Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006 PMID:...more information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different... analysis of CRISPR–Cas experiments. Nature Biotechnology , 38 (7), 813–823. https://doi.org/10.1038/s41587...
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  Adeno-associated virus (AAV) GuideTypeGuide...optical tools developed by researchers are used to detect, measure, and control molecular signals of cells... ddPCR AAV Titration by qPCR Using SYBR Green Technology References Burger, C., Gorbatyuk, O. S., Velardo...which together form the outer capsid shell that protects the viral genome. Helper plasmid E4 in trans Adenoviral...cell to release them. Optogenetics Biological technique to control the activity of cells, often neurons...
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  Educational ResourcesTypeGuide...’s repository , and related molecular biology techniques. Topics include: Genome Engineering Fluorescent...Fluorescent Proteins CRISPR Viral Vectors Plasmid Technology Career Readiness Molecular Biology Learn about plasmids...Genetic Code Strain Information Molecular Cloning Techniques Protocols Find written and video protocols for...
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  Modular Cloning GuideTypeGuide...Molecular Biology Reference Molecular Cloning Techniques MoClo Guide Modular Cloning (MoClo) Guide You...display of proteins of interest. Yeast Secrete and Detect Yeast Expression Sylvestre Marillonnet Plasmids...References and More Resources Guide to DNA Assembly Techniques by Nicola Patron at The Sainsbury Laboratory ...
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  Guide to Using Pooled LibrariesTypeGuide...so it’s essential that you have access to this technology if you’re planning to conduct a pooled library... reaction. As discussed above, access to this technology is crucial for conducting pooled library screens...
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  Lentiviral Vector GuideTypeGuide...accessibility (DIVA) identifies alterations in chromatin architecture through large-scale mapping of lentiviral integration...achieves efficient gene delivery in vivo. Nature Biotechnology , 15 (9), 871–875. https://doi.org/10.1038/nbt0997...
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  Addgene's eBook CollectionTypeGuide...nomenclature, and uses to common promoters and cloning techniques! Close About Overlay Select CRISPR 101 January...
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  Gamma-Retroviral Vector GuideTypeGuide... A. (2011). Gammaretroviral vectors: Biology, technology and application. Viruses , 3 (6), 677–713. https...
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  Adenovirus GuideTypeGuide...Ad35 vaccine vectors bypass immunity to Ad5 and protect nonhuman primates against ebolavirus challenge ...