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  2. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...for successful transduction. In another example, neuronal gene expression by an anterograde AAV was shown...input-defined neural pathways for defense behaviors." Neuron 93.1 (2017): 33-47. PubMed PMID: 27989459. PubMed...

  3. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...cells (as demonstrated in different cellular environments such as retina or glioblastoma). They also found.... Exo-AAV9 was successfully shown to transduce neurons in the brain after systemic (i.v.) delivery in ...

  4. Visualizing Genomic Loci with CRISPR-Sirius

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    Blog Post
    ...CRISPRainbow. Both tools were targeted to the FBN3 intronic repeat which contains 22 copies of the gRNA target...distances of loci pairs from intergenic regions, intronic regions, and a pericentromeric region. All pairs...

  5. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...effective delegation skills: The team will be stronger and less dependent on the manager to succeed. ... your team and take responsibility if things go wrong. Here are some important criteria for delegating...

  6. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... use amplicons in ITR as we have found this to strongly bias titer. We are also adopting ddPCR, which ...microglia? I have been using the AAV.PHP.eb for neuron, oligodendrocyte, and astrocyte specificity and...

  7. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann ...doi.org/10.1093/ilar.46.3.258  Mould AP, Askari JA, Byron A, Takada Y, Jowitt TA, Humphries MJ (2016) Ligand-induced...

  8. Viral Vectors 101: Viruses as Biological Tools

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    Blog Post
    ...immunogenicity, which means they do not stimulate a strong immune response when introduced into an organism...Harbor, NY, USA: 1997. PubMed PMID: 21433340. 4. Cronin J, et al. “Altering the tropism of lentiviral vectors...

  12. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...biosensing technique can be applied to an important neuronal signalling pathway - if you’ve got a useful tool...genes to specific cell types in the brain, mapping neuronal networks, and selectively activating signaling...

  13. CRISPR 101: Non-Homologous End Joining

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    Blog Post
    ...make a knockout in your gene of interest, as it is prone to generating indel errors. Indel errors generated...Non-Homologous End-Joining Really an Inherently Error-Prone Process? PLoS Genet 10:e1004086 . https://doi.org...

  14. 3 Challenges in Plant Synthetic Biology

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    Blog Post
    ...other than to sustain those techniques. We feel strongly that the scientific and the agricultural community...themselves if they want this beautiful flower in their front yard or not. By developing plant aesthetics with...

  16. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    Blog Post
    ...disease in question – cardiomyocytes, adipocytes or neurons, for instance – to see how those mutations influence...TALENs may be cleaner than CRISPR/Cas9. Reference: Quirong Ding et al. "A TALEN genome-editing system for ...

  18. Viral Vectors 101: An Introduction to AAV

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    Blog Post
    ...particularly helpful when wanting to target specific neuron subtypes, since AAVs tend to be very neuro-permissive...Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal circuits of the retina using viral tools.   References...

  19. All in a Twist: dsRNA

    Type
    Blog Post
    ...Transcription, translation, and prone to degradation – those are the words that describe RNA! Double ...telomeres and centromeres. These genomic regions are prone to persistent R-loop formation, presumably due to...

  20. RNA Extraction Without A Kit

    Type
    Blog Post
    ...degraded. To effectively isolate intact RNA, a fast, strong protein denaturant would be required- something...late 1970’s, Chirgwin and colleagues showed that a strong protein denaturant, guanidinium thiocyanate, did...
Showing: 381 - 400 of 671 results