Addgene

We narrowed to 414 results for: his

Showing: 381 - 400 of 414 results

Results per page:

Lentiviral Vector Uses and Overview

Type

Blog Post

...applications of lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers ...
Cpf1: A New Tool for CRISPR Genome Editing

Type

Blog Post

... off-target editing rates, determined using sophisticated genome-wide analysis. At many of its computationally...
AAV Titers: Where Do They Come From And What Do They Mean?

Type

Blog Post

...Quantitative PCR (qPCR) Digital droplet PCR (ddPCR) Historically, a quantitative DNA hybridization method (DNA...
New Tools Enable CRISPRa for Neuroscience Applications

Type

Blog Post

... visualizing neuronal differentiation by immunohistochemistry, it is not amenable to FACS. Thus, the ...
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

Type

Blog Post

...PMID: 7768854. PubMed Central PMCID: PMC177048. Thisted, Thomas, and Kenn Gerdes. "Mechanism of post-segregational...
Mesothelioma - Causes, Symptoms, and Treatment

Type

Blog Post

...brought a lot of promise to a field that has historically been full of bad news and dreadful prognoses...
Tips for Writing a Good Cover Letter

Type

Blog Post

...logistical, like briefly explaining a gap in your job history or sharing relocation plans. They can be skills-based...
Overcoming the AAV Size Limitation for CRISPR Delivery

Type

Blog Post

...post-injection. Livers from these mice were histologically normal and liver injury markers were not increased...
Finding nucleic acids with SHERLOCK and DETECTR

Type

Blog Post

...as well as health-related single nucleotide polymorphisms (SNPs) from human saliva. SHERLOCKv2 provides...
Antibodies 101: Immunoprecipitation

Type

Blog Post

...technique to identify DNA binding proteins involved in histone modification. Similarly, RNA IP (RIP) can be used...
To Codon Optimize or Not: That is the Question

Type

Blog Post

...are under expressed compared to others. Image from Hiss et al., 2017. Most commonly, scientists codon...
Neuronal labeling with Spaghetti Monster

Type

Blog Post

...protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific antibodies...
Getting the Most from Your Lentiviral Transduction

Type

Blog Post

...discarding and thawing a fresh vial. In many labs, the history of the cells in storage may be a bit dubious due...
An Integrin Antibody Toolkit from IPI

Type

Blog Post

...will also work in immunoprecipitation and immunohistochemistry. Table 1: IPI antibodies Antibody ...
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Type

Blog Post

...key to fitting large genes into AAV First, some history. In 2008, the Auricchio lab decided to solve the...
Antibodies 101: Introduction to Immunofluorescence

Type

Blog Post

...with IF are immunocytochemistry (ICC) or immunohistochemistry (IHC). All three of these terms refer to...
10 Steps to a Perfect Science Talk

Type

Blog Post

... like they’re sitting through a pre-recorded lecture.This takes practice (see above) and a deep familiarity...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

...HIV-infected reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription...
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Type

Blog Post

... 1. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO. Enzymatic assembly of DNA molecules...
Treating Muscular Dystrophy with CRISPR Gene Editing

Type

Blog Post

... blot, at <10% of the wild-type level. Muscle histology is improved, with vastly reduced inflammation ...

Showing: 381 - 400 of 414 results

Results per page:

Lentiviral Vector Uses and Overview

Cpf1: A New Tool for CRISPR Genome Editing

AAV Titers: Where Do They Come From And What Do They Mean?

New Tools Enable CRISPRa for Neuroscience Applications

Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

Mesothelioma - Causes, Symptoms, and Treatment

Tips for Writing a Good Cover Letter

Overcoming the AAV Size Limitation for CRISPR Delivery

Finding nucleic acids with SHERLOCK and DETECTR

Antibodies 101: Immunoprecipitation

To Codon Optimize or Not: That is the Question

Neuronal labeling with Spaghetti Monster

Getting the Most from Your Lentiviral Transduction

An Integrin Antibody Toolkit from IPI

Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Antibodies 101: Introduction to Immunofluorescence

10 Steps to a Perfect Science Talk

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Treating Muscular Dystrophy with CRISPR Gene Editing

Help Center

Deposit Plasmids

General Reagents

Viral Service

DNA Service

Plasmid Collections