We narrowed to 412 results for: his

1. Overcoming the AAV Size Limitation for CRISPR Delivery

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   ...post-injection. Livers from these mice were histologically normal and liver injury markers were not increased...

2. Finding nucleic acids with SHERLOCK and DETECTR

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   ...as well as health-related single nucleotide polymorphisms (SNPs) from human saliva. SHERLOCKv2 provides...

3. New Tools Enable CRISPRa for Neuroscience Applications

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   ... visualizing neuronal differentiation by immunohistochemistry, it is not amenable to FACS.  Thus, the ...

4. Antibodies 101: Immunoprecipitation

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   ...technique to identify DNA binding proteins involved in histone modification. Similarly, RNA IP (RIP) can be used...

5. Neuronal labeling with Spaghetti Monster

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   ...protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific antibodies...

6. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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   ...Plant Biology: New Tools for an Old Favorite A History of Genome Editing in Popular Culture Additional...

7. Tips for Writing a Good Cover Letter

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   ...logistical, like briefly explaining a gap in your job history or sharing relocation plans. They can be skills-based...

8. Mesothelioma - Causes, Symptoms, and Treatment

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   ...brought a lot of promise to a field that has historically been full of bad news and dreadful prognoses...

9. AAV Titers: Where Do They Come From And What Do They Mean?

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   ...Quantitative PCR (qPCR) Digital droplet PCR (ddPCR) Historically, a quantitative DNA hybridization method (DNA...

10. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ...PMID: 7768854. PubMed Central PMCID: PMC177048. Thisted, Thomas, and Kenn Gerdes. "Mechanism of post-segregational...

11. Getting the Most from Your Lentiviral Transduction

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    ...discarding and thawing a fresh vial. In many labs, the history of the cells in storage may be a bit dubious due...

12. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    ...key to fitting large genes into AAV First, some history. In 2008, the Auricchio lab decided to solve the...

13. Antibodies 101: Introduction to Immunofluorescence

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    ...with IF are immunocytochemistry (ICC) or immunohistochemistry (IHC). All three of these terms refer to...

14. An Integrin Antibody Toolkit from IPI

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    ...will also work in immunoprecipitation and immunohistochemistry. Table 1: IPI antibodies  Antibody ...

15. 10 Steps to a Perfect Science Talk

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    ... like they’re sitting through a pre-recorded lecture.This takes practice (see above) and a deep familiarity...

16. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    ...HIV-infected reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription...

17. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    ... 1. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO. Enzymatic assembly of DNA molecules...

18. Treating Muscular Dystrophy with CRISPR Gene Editing

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    ... blot, at <10% of the wild-type level. Muscle histology is improved, with vastly reduced inflammation ...

19. Plasmids 101: Repressible Promoters

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    ... GAL80 under different promoters and create sophisticated patterns of UAS-driven gene expression. To further...

20. Antibodies 101: The Basics of Western Blotting

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    ...and references References Aydin S (2015) A short history, principles, and types of ELISA, and our laboratory...