We narrowed to 542 results for: SHI

1. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

   Type

   Blog Post

   ...dSpyCas9) fused to APEX2 is expressed from a Tet/Shield1 inducible CMV promoter and targeted to a sequence...

2. In Vivo Biotinylation of Bacterial Fusion Proteins

   Type

   Blog Post

   ...immobilization on a solid support. This allows washing away of unbound material to calculate the amount...

3. The Golden Stopwatch Award: Recognizing Facilitators of Research

   Type

   Blog Post

   ...Pennsylvania  University of Utah University of Washington Vanderbilt University and Medical Center  Weizmann...

4. Single-cell tracking of lineage and identity with CellTag

   Type

   Blog Post

   ...reprogramming outcomes. CellTag from the Morris Lab at Washington University in St. Louis provides a combinatorial...

5. Viral Vectors 101: Transductions

   Type

   Blog Post

   ...logical choice for efficiency. Where viruses really shine is when a specific cell type within a live organism...

6. Viral Vectors 101: Parts of the AAV Transfer Plasmid

   Type

   Blog Post

   ...routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ITR loss, it’s best...

7. Summer SciComm Series: Master of SciComm

   Type

   Blog Post

   ...and passion on display in our building, each day brushing past and chatting with individuals whose research...

8. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

   Type

   Blog Post

   ...endogenous IgGs (Lu and Partridge, 1998). After washing away unbound fragments, you can then apply your...

9. Viral Vectors 101: What is a Virus (and a Viral Vector)?

   Type

   Blog Post

   ...Interactions (pp. 23–40). Springer International Publishing. https://doi.org/10.1007/978-3-319-07758-1_2 ...

10. Identifying Sequence Elements with SnapGene's Feature Database

    Type

    Blog Post

    ...what the user wants to do and make the path to accomplishing their goals as intuitive and painless as possible...

11. Addgene's Top 10 Blog Posts of 2015

    Type

    Blog Post

    ... we’ve experienced incredible growth with our readership expanding from ~10,000 users per month in November...

12. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type

    Blog Post

    ...heterologous genes, but the trend of research has shifted in recent years toward tinkering with regulatory...

13. Learning Technical Skills at a Lab Central Workshop

    Type

    Blog Post

    ... in addition to preparing DNA and generating/replenishing kits, is to find new ways to make lab processes...

14. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type

    Blog Post

    ... in Douglas Fowler's lab at the University of Washington sought to address the effects of genetic variants...

15. Improving Transduction Efficiency with Exosome AAVs

    Type

    Blog Post

    ..., et al. "Naturally enveloped AAV vectors for shielding neutralizing antibodies and robust gene delivery...

16. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type

    Blog Post

    ...by the dL5** FAP.  The iodine-modifications also shift MG-2I’s excitation wavelength to 666 nm, which is...

17. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type

    Blog Post

    ...thanks to our guest blogger Jon Chow. Jon Chow is finishing his immunology PhD at Harvard University. He has...

18. Addgene Depositors Get More Citations

    Type

    Blog Post

    ...citations in its first four years (1). In addition to publishing in Science, Professor Zhang deposited the associated...

19. Science Career Options

    Type

    Blog Post

    ... then decide on a course of action. Instead of rushing in, you take steps to make it more likely you’ll...

20. K. phaffii: Rising to the Occasion

    Type

    Blog Post

    ...an error-prone pathway frequently introducing frameshift knockout mutations. Thus, if genetic manipulation...