We narrowed to 1,006 results for: RING
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TypeBlog Post... text, we invite you to join us! We'll also be sharing our conference experience on X, Instagram, and ...
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The Codon Guide to Synthetic Biology
TypeBlog Post...editor of Asimov Press and is designing a genetic engineering curriculum at MIT. He also writes Codon, a blog... -
Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes
TypeBlog Post...will be required. If you have any questions on ordering plasmids, please feel free to email [email protected]... -
Expanded Access to Rabies Plasmids
TypeBlog Post...questions about this or other Addgene shipping and ordering processes, don’t hesitate to contact our friendly... -
Top Requested Plasmid of 2017 - pMD2.G
TypeBlog Post...production protocol on our website and tips for titering your lentivirus in another blog post. If you need... -
DIY DNA Ladders from Penn State University
TypeBlog Post...Get Tips on Verifying Your Plasmid Table 1: Comparing Typical Comericial Ladders with the PSU Ladders... -
New Educational Resource: The Addgene Videos Page
TypeBlog Post... some useful career advice. Our first video, featuring Outreach Scientist Jessica Welch, can be found... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog Post...biophysics of protein folding and selective pressures during protein evolution. The Baker Lab has deposited ... -
Podcast: A Malawian Professor's Path to Biotech Research
TypeBlog Post...University of Malawi where he focused on agricultural engineering through biotech. After graduating with a masters... -
PEI Calculator for Planning AAV Packaging Transfections
TypeBlog Post...you plan PEI transfections for AAV packaging, considering using this PEI Calculator. AAV packaging typically... -
#DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La
TypeBlog Post...December 31, 2021. Let us know which contest you are entering in (if you don’t include this, we will make our... -
Streaking for Single Colonies: The Streak Plate Challenge
TypeBlog Post...different strains of E. coli,” says Westenberg. During the transformation experiment, he plans to show... -
CRISPR Cheat Sheet
TypeBlog Post... The CRISPR field moves fast. That’s why we’re bringing you a brand new third edition of Addgene’s CRISPR... -
Countdown to Halloween @Addgene
TypeBlog Post...suspect that our costume contests were indeed occurring earlier but that the photo evidence was destroyed... -
2018 #DeckTheLab Contest Winners
TypeBlog Post...Heijmans There were many science-themed submissions: string lights hung up in the shape of RNA, light-up neurons... -
Performing In Vivo CRISPR Screens Using the FITS Approach
TypeBlog Post...Screening Using CRISPR Cancer and the Immune System: Deciphering the Relationship... -
Addgene’s Blog Gets a New Look!
TypeBlog Post...neuroscience), or explore other areas such as scientific sharing or publishing, under the Other dropdown menu. You... -
Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up
TypeBlog Post...of white space on this map and increase plasmid sharing across the globe! We hope that these awards will... -
Adeno-associated virus (AAV) Guide
TypeGuide...on transgene expression and the expression of neighboring genes. rAAV episomes can develop chromatin-like...infect. Serotypes are determined by naturally occurring differences in the variable regions of the virus...vector is determined by the packaging plasmid used during production, as it carries the Cap region responsible...AAV method which has shown higher efficiency in delivering large genes makes use of split inteins, proteins...proteins that carry out a naturally occurring process known as protein trans-splicing. As these methods are... initial infection. The risk of spontaneously occurring replication-competent rAAV vectors is extremely...evolution to vector revolution: use of naturally occurring serotypes of adeno-associated virus (AAV) as novel... -
What's New in CRISPR - Winter 2018
TypeBlog Post...created a modular toolkit for Cas-mediated genome engineering in plants. Read the paper in bioRxiv Find... -
Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6
TypeBlog Post... understanding bacterial antibiotic resistance. During this time, Dr. Koeris and Professor Timothy Lu ... -
Addgene's Website Update
TypeBlog Post...may notice some changes to our website! Part of sharing science and making access to materials easier is... -
Making Career Development an Integral Part of the Science Training System
TypeBlog Post...Massachusetts Medical School. It has been supported by a Steering Committee and working groups with representatives... -
Inntags: Innovative Protein Epitope Tagging
TypeBlog Post... Mary Gearing... -
Keeping Up With CRISPR/Cas9
TypeBlog Post...quoted as saying when the news first broke. "Considering that many human diseases are caused by genetic... -
Optogenetics Guide
TypeGuide...of optogenetics integrates optics and genetic engineering to measure and manipulate cells (frequently neurons...Scientists have identified a variety of naturally occurring microbial opsins that respond to different wavelengths...proton efflux. Researchers have used genetic engineering to improve these natural opsins - by inducing...direct stimulation of ion channels. Naturally occurring channelrhodopsins were discovered in the green...to place the optic fiber outside of the brain, rendering the experimental procedure less invasive. Different...LARIAT Inhibits protein function by reversibly sequestering targets into large, optically assembled protein...Illumination causes the photoswitch to unfold, lowering the toxin's local concentration near the cell ... -
Science Communication for Everyone
TypeBlog Post...For example, “manipulate” could mean “illicit tampering” to general audiences while “manipulate” could... -
CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats
TypeBlog Post...more-typical scale of tissue culture while still offering the potential for true biological insight. Pooled... -
Editor's Choice, August 2016
TypeBlog Post...post was contributed by Addgene Scientist Mary Gearing and discusses a newer, CRISPR-based technique to... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog Post...uncharacterized. This represents a considerable barrier to furthering research and developing effective treatments ... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog Post...immunotherapy targets but are embryonic lethal, hindering studies post embryogenesis. Using C-CHIME and ... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog Post...al. "Behavioural manipulation in a grasshopper harbouring hairworm: a proteomics approach." Proceedings... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog Post...including the reagent resources .csv file for easy filtering and sorting. Reference images can be viewed by... -
Future of Research Conference - Remarkable Opening Session
TypeBlog Post...increasing the value and priority put on good mentoring and applauded the new requirement for PIs to include... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post... a rapid covalent bond to a protein, through engineering a bacterial adhesin. Zakeri et al (Proc Natl ... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog Post...share their reagents. "We are proud to be able to bring such an important collection to the scientific community... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post...Medicine in New York recognized their limitations for peering right through living animals to see their organs... -
Molecular Biology Reference
TypeGuide...bacteria, which can uptake plasmids and amplify them during cell division. Because bacteria are easy to grow...that can drive expression of short RNAs. Genome Engineering Plasmids - Used to target and edit genomes. Genome...of animals. There are many different naturally occurring strains of E. coli , some of which are deadly ...Carbenicillin can be used in place of ampicillin. Preparing Antibiotics Create a stock solution of your antibiotic...Addgene recommends making 1000X stock solutions and storing aliquots at -20°C. To use, dilute your antibiotic...Specifically, A pairs with T and C pairs with G. During replication, DNA unwinds and the DNA polymerase...utilizes modified fluorescently tagged nucleotides. During illumina NGS, a long piece of DNA is first fragmented... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post...first collapse arg2 and then arg1 vesicles. By comparing ultrasound images pre-collapse and post-collapse... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog Post... can’t be reused without attribution, despite filtering for images labeled for reuse with modification...